Zobrazeno 1 - 4
of 4
pro vyhledávání: '"Nobutake Fugono"'
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 10 (2022)
Biosensors can be used for real-time monitoring of metabolites and high-throughput screening of producer strains. Use of biosensors has facilitated strain engineering to efficiently produce value-added compounds. Following our recent work on the prod
Externí odkaz:
https://doaj.org/article/04fccdca73c441a3bb7d3679b8f46f2a
Publikováno v:
FEMS Microbiology Letters. 192:237-241
We isolated a temperature-sensitive sporulation defective mutant of the sigA gene, encoding a major sigma factor, sigma(A) protein, in Bacillus subtilis, and designated it as sigA21. The sigA21 mutation caused a single-amino acid substitution, E314K,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bd2bb9f61a19f5512d3f6fd165bb2d77
https://doi.org/10.1111/j.1574-6968.2000.tb09388.x
https://doi.org/10.1111/j.1574-6968.2000.tb09388.x
Publikováno v:
Genetic analysis : biomolecular engineering. 15(1)
Taking advantage of highly conserved domains present in the secA gene from Escherichia coli and Bacillus subtilis, we designed degenerate oligonucleotides (oligos) corresponding to these regions. These oligos were used as primers in PCR in order to a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a1843b0e6feb3cb9565fdb5fcd64af29
https://pubmed.ncbi.nlm.nih.gov/10084122
https://pubmed.ncbi.nlm.nih.gov/10084122
Autor:
Yoko Asai, Yasurou Kurusu, Nobutake Fugono, Hideaki Yukawa, Masayuki Inui, Alain A. Vertès, Miki Kobayashi
Publikováno v:
Gene. 139(1)
A conserved domain of the secY genes from Bacillus subtilis, Mycoplasma capricolum and Escherichia coli was used to design degenerate oligodeoxyribonucleotides. These synthetic DNA sequences were used to screen a lambda library of Brevibacterium flav
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::146033649433d0b22a814ed5d5bc69b5
https://pubmed.ncbi.nlm.nih.gov/8112597
https://pubmed.ncbi.nlm.nih.gov/8112597