Zobrazeno 1 - 10
of 54
pro vyhledávání: '"Nobuichi Goto"'
Autor:
Takeshi Igarashi, Hidetoshi Arakawa, Koji Karasawa, Masako Maeda, Shigeya Suzuki, Nobuichi Goto
Publikováno v:
Analytical Biochemistry. 333:296-302
We developed a novel bioluminescent assay for detection of pyrophosphate in polymerase chain reaction (PCR) product. The principle of this method is as follows: pyrophosphate released by PCR is converted to adenosine 5'-triphosphate (ATP) by pyruvate
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e1ba7604b2594e45a37cb9bc0e711739
https://doi.org/10.1016/j.ab.2004.06.026
https://doi.org/10.1016/j.ab.2004.06.026
Publikováno v:
Oral Microbiology and Immunology. 19:102-105
In order to clarify the role that sortase (SrtA) plays in anchoring dextranase (Dex) to the cell wall of Streptococcus mutans, both Dex- and SrtA- mutants were constructed by insertional inactivation of the respective genes. Western blot analysis wit
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bbec6b7a4d5724c39b0cafb124decf04
https://doi.org/10.1046/j.0902-0055.2003.00123.x
https://doi.org/10.1046/j.0902-0055.2003.00123.x
Publikováno v:
Letters in Applied Microbiology. 35:223-227
Aims: To examine the dextran-binding domain of the dextranase (Dex) of Streptococcus mutans. Methods and Results: Deletion mutants of the Dex gene of Strep. mutans were prepared by polymerase chain reaction and expressed in Escherichia coli cells. Bi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4f2a44a541c8b9ea5edae679139171ac
https://doi.org/10.1046/j.1472-765x.2002.01160.x
https://doi.org/10.1046/j.1472-765x.2002.01160.x
Publikováno v:
Oral Microbiology and Immunology. 17:193-196
Dextranase (Dex) is an enzyme that hydrolyzes glucan, a polymer of glucose synthesized from sucrose by glucosyltransferases (GTFs). By comparing amino acid sequences of Dexs and GTFs, we found that the Dex enzymes of Streptococcus mutans, Streptococc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9f29463e40f9dcb8a3b1e81086084a6c
https://doi.org/10.1034/j.1399-302x.2002.170310.x
https://doi.org/10.1034/j.1399-302x.2002.170310.x
Publikováno v:
Journal of Microbiological Methods. 46:99-105
A pair of polymerase chain reaction (PCR) primers was designed on the basis of the nucleotide sequence homology of dextranase genes (dex) of Streptococcus mutans, S. sobrinus and S. downei. The primer pair amplified a 530-bp DNA fragment on the dex g
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::68ea5cf1f724041ac7717671b89d5d6c
https://doi.org/10.1016/s0167-7012(01)00263-9
https://doi.org/10.1016/s0167-7012(01)00263-9
Publikováno v:
Oral Microbiology and Immunology. 15:360-364
An oral parasite Trichomonas tenax ATCC 30207 synthesizes and secretes various proteinases. By gelatin-SDS-PAGE, we found five proteinases bands (30, 37, 46, 51 and 60 kDa) in cell lysate and four bands (37, 45, 52 and 60 kDa) in culture filtrate. Th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::34ea522b93791709c9c85fbc32b37302
https://doi.org/10.1034/j.1399-302x.2000.150604.x
https://doi.org/10.1034/j.1399-302x.2000.150604.x
Publikováno v:
Oral Microbiology and Immunology. 14:233-237
Three DNA fragments (SSB-1, -2 and -3) in the dextranase gene (dex) of Streptococcus sobrinus were amplified by polymerase chain reaction and used as DNA probes. The probes were examined for the specificity and the sensitivity of hybridization with D
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::641e35c2fc4fb8162fa7d3c416371167
https://doi.org/10.1034/j.1399-302x.1999.140406.x
https://doi.org/10.1034/j.1399-302x.1999.140406.x
Publikováno v:
Oral Microbiology and Immunology. 13:382-386
Thirty-one strains of 23 gram-negative oral bacterial species were examined for dextran-degrading activity on agar plates containing blue dextran. One strain each of Capnocytophaga ochracea, Capnocytophaga sputigena, Prevotella loescheii, Prevotella
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::154724b539d2a0ca63e39d63e7d4c152
https://doi.org/10.1111/j.1399-302x.1998.tb00696.x
https://doi.org/10.1111/j.1399-302x.1998.tb00696.x
Publikováno v:
Journal of Microbiological Methods. 34:81-88
Polymerase chain reaction (PCR) primers for dextranase genes ( dex ) were designed for rapid identification of oral streptococci. The primers specifically amplified a 1.2-kb DNA fragment in dex of the oral streptococcal species: Streptococcus mutans
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::72b56db5f09a452b6da7af5e0a4643a1
https://doi.org/10.1016/s0167-7012(98)00078-5
https://doi.org/10.1016/s0167-7012(98)00078-5
Autor:
Takashi Miyazaki, Takeshi Igarashi, Teruo Toko, Teuku Syafiuddin, Nobuichi Goto, Hisashi Hisamitsu, Akihiro Fujishima
Publikováno v:
Biomaterials. 18:1051-1057
Class V cavities were prepared and restored with resin composite containing antibacterial filler powder (Apacider-AW, Ap-AW) using experimental restorations. The restored teeth were incubated in vitro with the cariogenic bacteria Streptococcus mutans
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0f4ac95018671c0f5192b2366117ae2f
https://doi.org/10.1016/s0142-9612(97)88072-6
https://doi.org/10.1016/s0142-9612(97)88072-6