Zobrazeno 1 - 10
of 59
pro vyhledávání: '"Nobuaki Yanai"'
Publikováno v:
生活環境科学研究所研究報告 = Annual report of the Institute of Living and Environmental Sciences. 50:17-22
Autor:
Tomoka, Satoh, Nobuaki, Yanai
Publikováno v:
生活環境科学研究所研究報告 = Annual report of the Institute of Living and Environmental Sciences. 45:11-16
ジメチルスルホキシド(DMSO)は、培養哺乳類細胞凍結保存の凍結保護剤として一般的に用いられているが、DNAメチル化やヒストンの修飾によって、一部の細胞では分化を引き起こすこと
Autor:
Takeshi Sasaki, Masanori Seki, Masuo Obinata, Junichi Kameoka, Nobuaki Yanai, Hideo Harigae, Shinichiro Takahashi
Publikováno v:
Experimental Hematology. 34:519-527
Objective We previously established 33 bone marrow stromal cell lines from SV40 T-antigen transgenic mice. Of these, 27 clones supported erythroid colony formation, while 6 did not. The objective of this study is to identify the molecules that determ
Possible applications of conditionally immortalized tissue cell lines with differentiation functions
Autor:
Masuo Obinata, Nobuaki Yanai
Publikováno v:
Drug Delivery System. 18:86-94
生体の組織を構成している分化機能を持つ細胞は200種を超えると思われる. これら多様な細胞機能を解明し, 生体組織の機能特性を再構築するためには, 個々の分化機能を保持した細胞を
Autor:
Shoichiro Ohta, Masuo Obinata, Nobuaki Yanai, Hideki Fuse, Yoshiaki Tabuchi, Takashi Kondo, Shinji Asano
Publikováno v:
The Journal of Urology. :1538-1545
We developed and characterized a conditionally immortalized testicular Sertoli cell line from transgenic mice bearing the temperature sensitive simian virus 40 large T antigen gene pSVtsA58.Established cells from 8-week-old male transgenic mice were
Autor:
Keiko Totani, Makoto Suematsu, Nobuaki Yanai, Hideko Nagasawa, Yuzuru Ishimura, Reiko Agake-Suzuki, Masuo Obinata, Kuniaki Mukai, Fumiko Mitani
Publikováno v:
European Journal of Biochemistry. 269:69-81
To facilitate studies on differentiation of adrenocortical cells and regulation of steroidogenic genes, we established cell lines from adrenals of adult transgenic mice harboring a temperature-sensitive large T-antigen gene of simian virus 40. Adrena
Autor:
Nobuaki Yanai, Setsuko Hatakeyama, S. Hayashi, Masanobu Satoh, Masuo Obinata, Y. Ohara-Nemoto
Publikováno v:
Journal of Oral Pathology & Medicine. 30:296-304
We established two gingival epithelial cell lines (GE1 and GE6), originating from transgenic mice harboring the temperature-sensitive simian virus 40 large T-antigen gene. GE1 and GE6 grew at a permissive temperature (33 degrees C) in a pavement arra
Publikováno v:
Cell Structure and Function. 26:95-101
After detachment from the stromal cells, hematopoietic stem cells are thought to differentiate to the cytokine-dependent stages where their growth and differentiation are promoted by these cytokines. To examine the stromal regulation of hematopoietic
Autor:
Masatsugu Ueda, Sumio Ohtsuki, Tetsuya Terasaki, Hitomi Takanaga, Ken Ichi Hosoya, Nobuaki Yanai, Kazuhiro Tetsuka, Masuo Obinata
Publikováno v:
Cell Structure and Function. 26:197-203
To characterize acidic amino acid transport in type 2 astrocytes, we established conditionally immortalized rat astrocyte cell lines (TR-AST) from newly developed transgenic rats harboring temperature-sensitive SV40 large T-antigen gene. TR-AST exhib
Publikováno v:
Blood. 96:139-144
A new primitive hematopoietic cell line (THS119), exhibiting Lin−/Sca-1+/c-Kit+ a surface phenotype, grew and survived underneath stromal cells (TBR59). The ability of the THS119 cells to invade these stromal cell layers was dependent on the inclus