Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Noah Jakimo"'
Autor:
Lin Zhao, Sabrina R. T. Koseki, Rachel A. Silverstein, Nadia Amrani, Christina Peng, Christian Kramme, Natasha Savic, Martin Pacesa, Tomás C. Rodríguez, Teodora Stan, Emma Tysinger, Lauren Hong, Vivian Yudistyra, Manvitha R. Ponnapati, Joseph M. Jacobson, George M. Church, Noah Jakimo, Ray Truant, Martin Jinek, Benjamin P. Kleinstiver, Erik J. Sontheimer, Pranam Chatterjee
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-8 (2023)
Abstract CRISPR enzymes require a defined protospacer adjacent motif (PAM) flanking a guide RNA-programmed target site, limiting their sequence accessibility for robust genome editing applications. In this study, we recombine the PAM-interacting doma
Externí odkaz:
https://doaj.org/article/c6eaa1afc2b34657a91e037a58ba1052
Autor:
Colin McGaw, Anthony J. Garrity, Gabrielle Z. Munoz, Jeffrey R. Haswell, Sejuti Sengupta, Elise Keston-Smith, Pratyusha Hunnewell, Alexa Ornstein, Mishti Bose, Quinton Wessells, Noah Jakimo, Paul Yan, Huaibin Zhang, Lauren E. Alfonse, Roy Ziblat, Jason M. Carte, Wei-Cheng Lu, Derek Cerchione, Brendan Hilbert, Shanmugapriya Sothiselvam, Winston X. Yan, David R. Cheng, David A. Scott, Tia DiTommaso, Shaorong Chong
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-11 (2022)
Cas12i is a genome editing platform with compact size that fits in AAV vector with short 43-mer gRNA, absence of tracrRNA, ability to process pre-crRNA, and high specificity. Here the authors present an unbiased mutational scanning approach to engine
Externí odkaz:
https://doaj.org/article/b34236ad23cc4e1cb4a7c8a576cc399b
Autor:
Pranam Chatterjee, Jooyoung Lee, Lisa Nip, Sabrina R. T. Koseki, Emma Tysinger, Erik J. Sontheimer, Joseph M. Jacobson, Noah Jakimo
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-6 (2020)
Protospacer adjacent motif (PAM) requirements limit the target range of CRISPR endonucleases. Here, the authors graft the 5$$^{\prime}$$ ′ -NAAN-3$$^{\prime}$$ ′ PAM-interacting domain of SmacCas9 onto SpyCas9 to create adenine dinucleotide targe
Externí odkaz:
https://doaj.org/article/de6c445336ca4aacb34b61cb8daeb358
Autor:
Sivan Kaminski Strauss, Dvir Schirman, Ghil Jona, Aaron N Brooks, Aditya M Kunjapur, Alex N Nguyen Ba, Alice Flint, Andras Solt, Andreas Mershin, Atray Dixit, Avihu H Yona, Bálint Csörgő, Bede Phillip Busby, Bianca P Hennig, Csaba Pál, Daniel Schraivogel, Daniel Schultz, David G Wernick, Deepa Agashe, Dikla Levi, Dmitry Zabezhinsky, Dor Russ, Ehud Sass, Einat Tamar, Elad Herz, Emmanuel D Levy, George M Church, Idan Yelin, Iftach Nachman, Jeffrey E Gerst, Joseph M Georgeson, Katarzyna P Adamala, Lars M Steinmetz, Marc Rübsam, Markus Ralser, Michael Klutstein, Michael M Desai, Nilima Walunjkar, Ning Yin, Noa Aharon Hefetz, Noah Jakimo, Olga Snitser, Omri Adini, Prashant Kumar, Rachel Soo Hoo Smith, Razi Zeidan, Ronen Hazan, Roni Rak, Roy Kishony, Shannon Johnson, Shira Nouriel, Sibylle C Vonesch, Simmie Foster, Tal Dagan, Tanita Wein, Thrasyvoulos Karydis, Timothy M Wannier, Timothy Stiles, Viridiana Olin-Sandoval, William F Mueller, Yinon M Bar-On, Orna Dahan, Yitzhak Pilpel
Publikováno v:
PLoS Biology, Vol 17, Iss 3, p e3000182 (2019)
In experimental evolution, scientists evolve organisms in the lab, typically by challenging them to new environmental conditions. How best to evolve a desired trait? Should the challenge be applied abruptly, gradually, periodically, sporadically? Sho
Externí odkaz:
https://doaj.org/article/bb9a50be8dcc4dc98274ddfdc59bba88
Autor:
Erik J. Sontheimer, Emma Tysinger, Noah Jakimo, Jooyoung Lee, Pranam Chatterjee, Lisa Nip, Sabrina R. T. Koseki, Joseph M. Jacobson
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-6 (2020)
Nature Communications
Nature Communications
CRISPR-associated (Cas) DNA-endonucleases are remarkably effective tools for genome engineering, but have limited target ranges due to their protospacer adjacent motif (PAM) requirements. We demonstrate a critical expansion of the targetable sequence
Programmable CRISPR enzymes are powerful and versatile tools for genome editing. They, however, require a specific protospacer adjacent motif (PAM) flanking the target site, which constrains the accessible sequence space for position-specific genome
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1b24817b85c741d2c9c0095494beaca9
Autor:
David G. Wernick, Michael Klutstein, Roni Rak, Shannon M. Johnson, Andreas Mershin, Daniel Schultz, Simmie L. Foster, Sibylle C. Vonesch, Yinon M. Bar-On, Daniel Schraivogel, Jeffrey E. Gerst, Olga Snitser, Yitzhak Pilpel, Katarzyna P. Adamala, Dmitry Zabezhinsky, Idan Yelin, Atray Dixit, Markus Ralser, Bianca P. Hennig, Orna Dahan, Dvir Schirman, Dikla Levi, Aditya M. Kunjapur, Roy Kishony, Sivan Kaminski Strauss, Bede P. Busby, Joseph M. Georgeson, Omri Adini, Ghil Jona, Alex N. Nguyen Ba, Ronen Hazan, Shira Nouriel, Lars M. Steinmetz, Timothy Stiles, Tal Dagan, Timothy M. Wannier, William F. Mueller, Thrasyvoulos Karydis, Csaba Pál, Ehud Sass, Noah Jakimo, Marc Rübsam, George M. Church, Alice Flint, Tanita Wein, Prashant Kumar, Nilima Walunjkar, Viridiana Olin-Sandoval, Aaron N. Brooks, Dor Russ, Andras Solt, Iftach Nachman, Einat Shaer Tamar, Michael M. Desai, Bálint Csörgő, Noa Aharon Hefetz, Rachel Soo Hoo Smith, Deepa Agashe, Razi Zeidan, Ning Yin, Emmanuel D. Levy, Elad Herz, Avihu H. Yona
Publikováno v:
PLoS Biology
PLoS Biology, Vol 17, Iss 3, p e3000182 (2019)
PLoS Biology, Vol 17, Iss 3, p e3000182 (2019)
In experimental evolution, scientists evolve organisms in the lab, typically by challenging them to new environmental conditions. How best to evolve a desired trait? Should the challenge be applied abruptly, gradually, periodically, sporadically? Sho
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::32c2152f7a9827795b86493d0005a3c5
Autor:
Noah Jakimo, Tomás Rodríguez, Pranam Chatterjee, Sabrina R. T. Koseki, Jooyoung Lee, Rui Qing, Emma Tysinger, Nadia Amrani, Erik J. Sontheimer, Joseph M. Jacobson, Shilei Hao
Publikováno v:
Nature Biotechnology. 38:1212-1212
Publikováno v:
Science Advances
S. canis Cas9 is a natural CRISPR enzyme that uses two motif insertions to enable flexible targeting of DNA sequences.
RNA-guided DNA endonucleases of the CRISPR-Cas system are widely used for genome engineering and thus have numerous applicatio
RNA-guided DNA endonucleases of the CRISPR-Cas system are widely used for genome engineering and thus have numerous applicatio
CRISPR-associated (Cas) DNA-endonucleases are remarkably effective tools for genome engineering, but have limited target ranges due to their protospacer adjacent motif (PAM) requirements. We demonstrate a critical expansion of the targetable sequence
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::436438b313b2c787ceb8537bef82b8c1