An improved reporter identifies ruxolitinib as a potent and cardioprotective CaMKII inhibitor.

Autor: Reyes Gaido OE; Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA., Pavlaki N; Department of Cardiology, Boston Children's Hospital and Harvard Medical School, Boston, MA 02115, USA., Granger JM; Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA., Mesubi OO; Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA., Liu B; Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA., Lin BL; Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA., Long A; Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA., Walker D; Department of Cardiology, Boston Children's Hospital and Harvard Medical School, Boston, MA 02115, USA., Mayourian J; Department of Cardiology, Boston Children's Hospital and Harvard Medical School, Boston, MA 02115, USA., Schole KL; Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA., Terrillion CE; Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA., Nkashama LJ; Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA., Hulsurkar MM; Cardiovascular Research Institute and Department of Integrative Physiology, Baylor College of Medicine, Houston, TX 77030, USA., Dorn LE; Cardiovascular Research Institute and Department of Integrative Physiology, Baylor College of Medicine, Houston, TX 77030, USA., Ferrero KM; Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA., Huganir RL; Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA., Müller FU; Institute of Pharmacology and Toxicology, University of Münster, Münster 48149, Germany., Wehrens XHT; Cardiovascular Research Institute and Department of Integrative Physiology, Baylor College of Medicine, Houston, TX 77030, USA.; Departments of Medicine, Neuroscience, and Pediatrics, Center for Space Medicine, Baylor College of Medicine, Houston, TX 77030, USA., Liu JO; Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA., Luczak ED; Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA., Bezzerides VJ; Department of Cardiology, Boston Children's Hospital and Harvard Medical School, Boston, MA 02115, USA., Anderson ME; Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.; Division of Biological Sciences and the Pritzker School of Medicine, University of Chicago, Chicago, IL 60637, USA.

Publikováno v: Science translational medicine [Sci Transl Med] 2023 Jun 21; Vol. 15 (701), pp. eabq7839. Date of Electronic Publication: 2023 Jun 21.

WNK kinases sense molecular crowding and rescue cell volume via phase separation.

Autor: Boyd-Shiwarski CR; Department of Medicine, Renal-Electrolyte Division, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA; Pittsburgh Center for Kidney Research, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA., Shiwarski DJ; Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA 15213, USA., Griffiths SE; Department of Medicine, Renal-Electrolyte Division, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA., Beacham RT; Department of Medicine, Renal-Electrolyte Division, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA., Norrell L; Molecular Medicine Program, University of Utah, Salt Lake City, UT 84132, USA., Morrison DE; Molecular Medicine Program, University of Utah, Salt Lake City, UT 84132, USA., Wang J; Department of Neurology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA., Mann J; Department of Neurobiology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA., Tennant W; Department of Neurobiology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA., Anderson EN; Department of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA., Franks J; Center for Biological Imaging, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA., Calderon M; Center for Biological Imaging, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA., Connolly KA; Department of Medicine, Renal-Electrolyte Division, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA., Cheema MU; Department of Medicine, Renal-Electrolyte Division, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA., Weaver CJ; Department of Medicine, Renal-Electrolyte Division, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA., Nkashama LJ; Department of Medicine, Renal-Electrolyte Division, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA., Weckerly CC; Department of Cell Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA., Querry KE; Department of Medicine, Renal-Electrolyte Division, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA., Pandey UB; Department of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA; Center for Protein Conformational Diseases, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA., Donnelly CJ; Department of Neurobiology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA; Center for Protein Conformational Diseases, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA., Sun D; Department of Neurology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA; VA Pittsburgh Healthcare System, Pittsburgh, PA 15240, USA., Rodan AR; Department of Human Genetics, University of Utah, Salt Lake City, UT 84132, USA; Molecular Medicine Program, University of Utah, Salt Lake City, UT 84132, USA; Department of Internal Medicine, Division of Nephrology and Hypertension, University of Utah, Salt Lake City, UT 84132, USA; Medical Service, VA Salt Lake City Health Care System, Salt Lake City, UT 84148, USA., Subramanya AR; Department of Medicine, Renal-Electrolyte Division, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA; Department of Cell Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA; Center for Protein Conformational Diseases, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA; Pittsburgh Center for Kidney Research, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA; VA Pittsburgh Healthcare System, Pittsburgh, PA 15240, USA. Electronic address: ars129@pitt.edu.

Publikováno v: Cell [Cell] 2022 Nov 23; Vol. 185 (24), pp. 4488-4506.e20. Date of Electronic Publication: 2022 Oct 31.

L-WNK1 is required for BK channel activation in intercalated cells.

Autor: Ray EC; Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania., Carrisoza-Gaytan R; Department of Pediatrics, Icahn School of Medicine at Mount Sinai, New York, New York., Al-Bataineh M; Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania., Marciszyn AL; Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania., Nkashama LJ; Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania., Chen J; Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania., Winfrey A; Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania., Griffiths S; Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania., Lam TR; Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania., Flores D; Department of Pediatrics, Icahn School of Medicine at Mount Sinai, New York, New York., Wu P; Department of Pharmacology, New York Medical College, Valhalla, New York., Wang W; Department of Pharmacology, New York Medical College, Valhalla, New York., Huang CL; Department of Internal Medicine, University of Iowa Carver College of Medicine, Iowa City, Iowa., Subramanya AR; Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania.; Department of Cell Biology, University of Pittsburgh, Pittsburgh, Pennsylvania., Kleyman TR; Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania.; Department of Cell Biology, University of Pittsburgh, Pittsburgh, Pennsylvania.; Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, Pennsylvania., Satlin LM; Department of Pediatrics, Icahn School of Medicine at Mount Sinai, New York, New York.

Publikováno v: American journal of physiology. Renal physiology [Am J Physiol Renal Physiol] 2021 Aug 01; Vol. 321 (2), pp. F245-F254. Date of Electronic Publication: 2021 Jul 06.

Intercalated cell BKα subunit is required for flow-induced K+ secretion.

Autor: Carrisoza-Gaytan R; Department of Pediatrics, Icahn School of Medicine at Mount Sinai, New York, New York, USA., Ray EC; Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Flores D; Department of Pediatrics, Icahn School of Medicine at Mount Sinai, New York, New York, USA., Marciszyn AL; Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Wu P; Department of Pharmacology, New York Medical College, Valhalla, New York, USA., Liu L; McGill University, Montreal, Quebec, Canada., Subramanya AR; Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.; Department of Cell Biology and., Wang W; Department of Pharmacology, New York Medical College, Valhalla, New York, USA., Sheng S; Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Nkashama LJ; Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Chen J; Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Jackson EK; Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Mutchler SM; Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.; Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Heja S; Department of Pediatrics, Icahn School of Medicine at Mount Sinai, New York, New York, USA., Kohan DE; Department of Medicine, University of Utah Health Sciences Center, Salt Lake City, Utah, USA., Satlin LM; Department of Pediatrics, Icahn School of Medicine at Mount Sinai, New York, New York, USA., Kleyman TR; Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.; Department of Cell Biology and.; Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.

Publikováno v: JCI insight [JCI Insight] 2020 Apr 07; Vol. 5 (8). Date of Electronic Publication: 2020 Apr 07.