Microtubule organization within mitotic spindles revealed by serial block face scanning electron microscopy and image analysis

Autor: Nixon, Faye M., Honnor, Thomas R., Clarke, Nicholas I., Starling, Georgina P., Beckett, Alison J., Johansen, Adam M., Brettschneider, Julia A., Prior, Ian A., Royle, Stephen J.

Publikováno v: Journal of Cell Science

Serial block face scanning electron microscopy (SBF-SEM) is a powerful method to analyze cells in 3D. Here, working at the resolution limit of the method, we describe a correlative light-SBF-SEM workflow to resolve microtubules of the mitotic spindle

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::b0d5aee0433ba0f1bd735216f7cf303f
http://livrepository.liverpool.ac.uk/3007019/1/Nixon2017R.pdf

Microtubule organization within mitotic spindles revealed by serial block face scanning electron microscopy and image analysis.

Autor: Nixon FM; Centre for Mechanochemical Cell Biology, Warwick Medical School, Coventry CV4 7AL, UK.; Institute for Translational Medicine, University of Liverpool L69 3BX, UK., Honnor TR; Department of Statistics, University of Warwick, Coventry CV4 7AL, UK., Clarke NI; Centre for Mechanochemical Cell Biology, Warwick Medical School, Coventry CV4 7AL, UK., Starling GP; Centre for Mechanochemical Cell Biology, Warwick Medical School, Coventry CV4 7AL, UK., Beckett AJ; Institute for Translational Medicine, University of Liverpool L69 3BX, UK., Johansen AM; Department of Statistics, University of Warwick, Coventry CV4 7AL, UK., Brettschneider JA; Department of Statistics, University of Warwick, Coventry CV4 7AL, UK., Prior IA; Institute for Translational Medicine, University of Liverpool L69 3BX, UK., Royle SJ; Centre for Mechanochemical Cell Biology, Warwick Medical School, Coventry CV4 7AL, UK s.j.royle@warwick.ac.uk.

Publikováno v: Journal of cell science [J Cell Sci] 2017 May 15; Vol. 130 (10), pp. 1845-1855. Date of Electronic Publication: 2017 Apr 07.