Zobrazeno 1 - 10
of 44
pro vyhledávání: '"Nin-Nin Chuang"'
Autor:
Chien-Wen Chen, Ya-Nan Chang, Yao-Sheng Chen, Chiao-Hwa Yeh, Kuo-Wen Pan, Chi-Yao Chang, Nin-Nin Chuang, Chung-Hsiang Yuan, Ming-Shan Wu
Publikováno v:
Biochemical and Biophysical Research Communications. 372:674-680
Grouper iridovirus (GIV) is one of the most devastating infectious pathogens of aquaculture fish. When infecting a susceptible cell line, such as GK-2, GIV causes antigenic changes in host cellular proteins. To understand the host gene expression cha
Autor:
Nin-Nin Chuang, Jin-Wen Huang
Publikováno v:
Biochemical and Biophysical Research Communications. 350:227-232
Syndecan-2 was found to detach from RACK1 and associate with caveolin-2 and Ras in cells transformed with oncogenic ras. Most of syndecan-2 from transformed cells was revealed with negligible phosphorylations at tyrosine residues. We experimented wit
Publikováno v:
Biochemical and Biophysical Research Communications. 330:1087-1094
HiTrap-syndecan-2/p120-GAP and HiTrap-syndecan-2/RACK1 affinity columns were applied to reveal that Src tyrosine kinase was highly expressed in BALB/3T3 cells transfected with plasmids pcDNA3.1-[S-ras(Q(61)K)] of shrimp Penaeus japonicus. Both column
Publikováno v:
Biochemical and Biophysical Research Communications. 329:855-862
BALB/3T3 cells transfected with plasmids pcDNA3.1-[S-ras(Q61K)] of shrimp Penaeus japonicus were applied to reveal a complex of p120-GAP/syndecan-2 being highly expressed upon transformation. Of interest, most of the p120-GAP/syndecan-2 complex was l
Publikováno v:
Journal of Experimental Zoology. :307-316
Phosphotyrosyl protein phosphatase (PTPase) 1B was purified from human placenta. Immunoprecipitation analysis revealed that the isolated PTPase 1B appears as a complex with the receptor for protein kinase C (RACK1) and protein kinase C (PKC)delta. Th
Publikováno v:
Journal of Experimental Zoology. :119-127
Receptors for activated C kinase (RACKs) are a group of protein kinase C (PKC) binding proteins that have been shown to be crucial in the translocation and subsequent functioning of PKC on activation. RACK1 isolated from BALB/3T3 cells transformed wi
Autor:
Kai‐Chun Chang, Nin-Nin Chuang
Publikováno v:
Journal of Experimental Zoology. 290:642-651
BALB/3T3 cells were transformed by transfection with DNA encoding the mutated ras(Q(61)K) from shrimp Penaeus japonicus (Huang et al., 2000). The GTPase-activating protein (GAP) in the cytosol fraction was significantly expressed and degraded, compar
Publikováno v:
Journal of Experimental Zoology. 289:441-448
BALB/3T3 cells were transformed by transfection with DNA encoding the mutated ras(Q(61)K) from shrimp Penaeus japonicus. Ras transcription and protein levels had increased significantly in the cells transfected with the S-ras plasmid, compared to cel
Autor:
Nin-Nin Chuang, Chein-Fuang Huang
Publikováno v:
Journal of Experimental Zoology. 286:441-449
In order to assess the effects of guanine nucleotide binding on the geranylgeranylation at the CAAX box of the shrimp Ras, we experimented with the shrimp Penaeus japonicus Ras (S-Ras) which is geranylgeranylated at the C-termini, shares 85% homology
Differential expression of ras in organs and embryos of shrimp Penaeus monodon (Crustacea: Decapoda)
Publikováno v:
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 125:307-315
Total RNA from shrimp hepatopancreas of Penaeus monodon showed three prominent bands that react with the shrimp ras probe, a 239-bp product, of approximately 4.8 kb (R1), 3.1 kb (R2) and 1.3 kb (R3) on the northern blot. The R1 is the least abundant.