Comparison of extraction methods for intracellular metabolomics of human tissues

Autor: Carolin Andresen, Tobias Boch, Hagen M. Gegner, Nils Mechtel, Andreas Narr, Emrullah Birgin, Erik Rasbach, Nuh Rahbari, Andreas Trumpp, Gernot Poschet, Daniel Hübschmann

Publikováno v: Frontiers in Molecular Biosciences, Vol 9 (2022)

Analyses of metabolic compounds inside cells or tissues provide high information content since they represent the endpoint of biological information flow and are a snapshot of the integration of many regulatory processes. However, quantification of t

Externí odkaz: https://doaj.org/article/62facc52ca3149f7aad06d434be45a5f

Oxygen Gradient Induced in Microfluidic Chips Can Be Used as a Model for Liver Zonation

Autor: Shahrouz Ghafoory, Christina Stengl, Stefan Kopany, Mert Mayadag, Nils Mechtel, Brennah Murphy, Sebastian Schattschneider, Niklas Wilhelmi, Stefan Wölfl

Publikováno v: Cells, Vol 11, Iss 23, p 3734 (2022)

Availability of oxygen plays an important role in tissue organization and cell-type specific metabolism. It is, however, difficult to analyze hypoxia-related adaptations in vitro because of inherent limitations of experimental model systems. In this

Externí odkaz: https://doaj.org/article/af6083077b3b4f47bbabfdaf9e0ea800

Deep metabolic profiling assessment of tissue extraction protocols for three model organisms

Autor: Hagen M. Gegner, Nils Mechtel, Elena Heidenreich, Angela Wirth, Fabiola Garcia Cortizo, Katrin Bennewitz, Thomas Fleming, Carolin Andresen, Marc Freichel, Aurelio Teleman, Jens Kroll, Rüdiger Hell, Gernot Poschet

Metabolic profiling harbors the potential to better understand various disease entities such as cancer, diabetes, Alzheimer’s, Parkinson’s disease or COVID-19. Deciphering these intricate pathways in human studies requires large sample sizes as a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::91a93f123bb1c96226076b5961bb34c7
https://doi.org/10.1101/2021.12.16.472947

A Novel UPLC-MS/MS Method Identifies Organ-Specific Dipeptide Profiles

Autor: Georg F. Hoffmann, Gernot Poschet, Verena Peters, Peter P. Nawroth, Nils Mechtel, Elena Heidenreich, Tamara Kracke, Rüdiger Hell, Tilman Pfeffer, Claus Peter Schmitt

Publikováno v: International Journal of Molecular Sciences
Volume 22
Issue 18
International Journal of Molecular Sciences, Vol 22, Iss 9979, p 9979 (2021)
Int. J. Mol. Sci. 22:9979 (2021)

Background: Amino acids have a central role in cell metabolism, and intracellular changes contribute to the pathogenesis of various diseases, while the role and specific organ distribution of dipeptides is largely unknown. Method: We established a se

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01bfb8355990de8d7d23b6c53372704b