Conformational heterogeneity coupled with β-fibril formation of a scaffold protein involved in chronic mental illnesses

Autor: Abhishek Cukkemane, Nina Becker, Mara Zielinski, Benedikt Frieg, Nils-Alexander Lakomek, Henrike Heise, Gunnar F. Schröder, Dieter Willbold, Oliver H. Weiergräber

Publikováno v: Translational Psychiatry, Vol 11, Iss 1, Pp 1-13 (2021)

Abstract Chronic mental illnesses (CMIs) pose a significant challenge to global health due to their complex and poorly understood etiologies and hence, absence of causal therapies. Research of the past two decades has revealed dysfunction of the disr

Externí odkaz: https://doaj.org/article/0c11b45bb5c841caa492b0d561904b9f

Phage Display-Derived Compounds Displace hACE2 from Its Complex with SARS-CoV-2 Spike Protein

Autor: Marc Sevenich, Elena Thul, Nils-Alexander Lakomek, Thomas Klünemann, Maren Schubert, Federico Bertoglio, Joop van den Heuvel, Patrick Petzsch, Jeannine Mohrlüder, Dieter Willbold

Publikováno v: Biomedicines, Vol 10, Iss 2, p 441 (2022)

Severe respiratory syndrome coronavirus-2 (SARS-CoV-2) is a highly contagious beta-class coronavirus. Although vaccinations have shown high efficacy, the emergence of novel variants of concern (VOCs) has already exhibited traits of immune evasion. Th

Externí odkaz: https://doaj.org/article/a30d7e10cf784031bebef2c32e46f490

Intrinsic Disorder of the Neuronal SNARE Protein SNAP25a in its Pre-fusion Conformation

Autor: Tobias Stief, Lothar Gremer, Sonja Pribicevic, Delane F. Espinueva, Katharina Vormann, Ralf Biehl, Reinhard Jahn, Ángel Pérez-Lara, Nils-Alexander Lakomek

Publikováno v: Journal of Molecular Biology. 435:168069

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::70661c0505c44b0adb21c23134d9d20e
https://doi.org/10.1016/j.jmb.2023.168069

Dimer Organization of Membrane-Associated NS5A of Hepatitis C Virus as Determined by Highly Sensitive 1 H-Detected Solid-State NMR

Autor: Alexander A. Malär, Alons Lends, Susanne Penzel, Anja Böckmann, Ralf Bartenschlager, Beat H. Meier, Vlastimil Jirasko, Marco E. Weber, Marie-Laure Fogeron, Nils-Alexander Lakomek

Publikováno v: Angewandte Chemie International Edition
Angewandte Chemie International Edition, Wiley-VCH Verlag, 2021, 60 (10), pp.5339-5347. ⟨10.1002/anie.202013296⟩
Angewandte Chemie

International audience; The Hepatitis C virus nonstructural protein 5A (NS5A) is a membrane-associated protein involved in multiple steps of the viral life cycle. Direct-acting antivirals (DAAs) targeting NS5A are a cornerstone of antiviral therapy,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ac0fa34117977f0fffbeab0a07130c3d
https://hal.archives-ouvertes.fr/hal-03366997/document

Proton-Detected Solid-State NMR of the Cell-Free Synthesized a-Helical Transmembrane Protein NS4B from Hepatitis C Virus

Autor: Marie-Laure Fogeron, Vlastimil Jirasko, Beat H. Meier, Ralf Bartenschlager, Nils-Alexander Lakomek, Susanne Penzel, Anja Böckmann

Publikováno v: ChemBioChem
ChemBioChem, Wiley-VCH Verlag, In press, ⟨10.1002/cbic.201900765⟩
Chembiochem
ChemBioChem, 21 (10)

Proton‐detected 100 kHz magic‐angle‐spinning (MAS) solid‐state NMR is an emerging analysis method for proteins with only hundreds of microgram quantities, and thus allows structural investigation of eukaryotic membrane proteins. This is the c

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1364ee43a11bde30333b59e7ea83de52
https://hal.archives-ouvertes.fr/hal-02569380/file/BA226B08-4C22-41FF-9B92-3C47625FD2DE.pdf