Zobrazeno 1 - 10
of 42
pro vyhledávání: '"Nilima Sarkar"'
Publikováno v:
IUBMB Life. 42:211-215
In the light of recent evidence that the half-life of bacterial mRNA may be modulated by polyadenylation at the 3' end, we determined the half-life of polyadenylated lpp mRNA, which is an abundant and comparatively stable message encoding a major lip
Autor:
Nilima Sarkar
Publikováno v:
Microbiology. 142:3125-3133
Autor:
Nilima Sarkar, Gong-jie Cao
Publikováno v:
FEMS Microbiology Letters. 108:281-285
Using improved procedures for the synthesis, amplification, and cloning of DNA complementary to bacterial poly(A) RNA, we succeeded in deducing the 3′-terminal sequence of polyadenylylated flagellin mRNA encoded by the Bacillus subtilis hag gene. T
Autor:
Gong-jie Cao, Nilima Sarkar
Publikováno v:
Biochemical and biophysical research communications. 239(1)
Polyadenylation of Escherichia coli specific mRNAs has so far been studied primarily during the exponential phase of growth. As part of an investigation of the polyadenylation of E. coli mRNAs in different physiological contexts, we studied mRNA poly
Publikováno v:
Biochemistry and molecular biology international. 41(5)
Two poly(A) polymerase activities were identified in extracts of a strain of Bacillus subtilis in which the gene for polynucleotide phosphorylase was disrupted. Gel filtration studies showed a large difference in the molecular size of the two poly(A)
Autor:
Nilima Sarkar
Publikováno v:
Annual review of biochemistry. 66
The 3′-ends of both prokaryotic and eukaryotic mRNA are polyadenylated, but the poly(A) tracts of prokaryotic mRNA are generally shorter, ranging from 15 to 60 adenylate residues and associated with only 2–60% of the molecules of a given mRNA spe
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America. 93(21)
We had earlier identified the pcnB locus as the gene for the major Escherichia coli poly(A) polymerase (PAP I). In this report, we describe the disruption and identification of a candidate gene for a second poly(A) polymerase (PAP II) by an experimen
Publikováno v:
DNA and cell biology. 14(12)
We have developed a permeable cell system for the study of the molecular mechanisms involved in the control and initiation of DNA replication at the origin of the Bacillus subtilis chromosome. Our system take advantage of the synchronous initiation o
Effect of mercury substitution of DNA on its susceptibility to cleavage by restriction endonucleases
Autor:
Nilima Sarkar, Gaspar Banfalvi
Publikováno v:
DNA and cell biology. 14(5)
Mercurated DNA was synthesized in vitro by substituting Hg-dCTP or Hg-dUMP for dCTP in one strand of M13mp8DNA in a DNA polymerase I reaction. Restriction enzymes, including Sma I, Pst I, Bam HI, Hind III, and Hinc II, were completely inactive when t
Publikováno v:
American journal of obstetrics and gynecology. 170(2)
OBJECTIVES: Our purpose was to develop a sensitive method for assessing the replication time of specific human genes in cultured fetal cells and for detecting potential replication defects. STUDY DESIGN: Synchronous progression of diploid human fetal