Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Nikolay A. Aleksashin"'
Autor:
Shijie Huang, Nikolay A. Aleksashin, Anna B. Loveland, Dorota Klepacki, Kaspar Reier, Amira Kefi, Teresa Szal, Jaanus Remme, Luc Jaeger, Nora Vázquez-Laslop, Andrei A. Korostelev, Alexander S. Mankin
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-13 (2020)
Ribosomes of all organisms have retained 5S rRNA as an autonomous rRNA species. Here the authors engineer a bacterial strain with ribosomes that do not have free 5S rRNA, and carry structural analyses that suggest the evolutionary preservation of 5S
Externí odkaz:
https://doaj.org/article/97ce74dddff14abd9b4d20a9c96f526d
Autor:
Nikolay A. Aleksashin, Teresa Szal, Anne E. d’Aquino, Michael C. Jewett, Nora Vázquez-Laslop, Alexander S. Mankin
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
Ribosome engineering is an emerging powerful approach for synthetic protein synthesis. Here the authors invert the Ribo-T system, using the engineered ribosome to translate the proteome while the native ribosome translates specific mRNA.
Externí odkaz:
https://doaj.org/article/2fa4b8a9829c4319b8a0ec1c4381a811
Autor:
Nikolay A. Aleksashin, Margus Leppik, Adam J. Hockenberry, Dorota Klepacki, Nora Vázquez-Laslop, Michael C. Jewett, Jaanus Remme, Alexander S. Mankin
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
The tethered ribosome system Ribo-T supports cell proliferation though at a reduced rate. Here the authors show this is due to slower ribosome assembly instead of reduced functionality.
Externí odkaz:
https://doaj.org/article/dd41dd2510804c7598e1969fe536f5bc
Publikováno v:
Frontiers in Molecular Biosciences, Vol 5 (2018)
The ribosome is one of the major targets in the cell for clinically used antibiotics. However, the increase in multidrug resistant bacteria is rapidly reducing the effectiveness of our current arsenal of ribosome-targeting antibiotics, highlighting t
Externí odkaz:
https://doaj.org/article/2a97aa2934e94634a798f15dc96a52f2
Publikováno v:
bioRxiv
Cell-free protein synthesis (CFPS) systems enable easyin vitroexpression of proteins with many scientific, industrial, and therapeutic applications. Here we present an optimized, highly efficient human cell-free translation system that bypasses many
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d39b3d0a2bc74b4f35a340d5ed53c6f4
https://doi.org/10.1101/2023.02.09.527910
https://doi.org/10.1101/2023.02.09.527910
Autor:
Alexander S. Mankin, Nora Vázquez-Laslop, Anne E. d’Aquino, Nikolay A. Aleksashin, Michael C. Jewett, Teresa Szal
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
Nature Communications
Nature Communications
Ribosome engineering is a powerful approach for expanding the catalytic potential of the protein synthesis apparatus. Due to the potential detriment the properties of the engineered ribosome may have on the cell, the designer ribosome needs to be fun
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c359b1e8f19de1b53d122cdf6f38d6c5
https://doaj.org/article/2fa4b8a9829c4319b8a0ec1c4381a811
https://doaj.org/article/2fa4b8a9829c4319b8a0ec1c4381a811
Autor:
Antje Krüger, Tasfia Azim, Adam J. Hockenberry, Anne E. d’Aquino, Michael C. Jewett, Nikolay A. Aleksashin
Publikováno v:
Nucleic Acids Research
The synthetic capability of the Escherichia coli ribosome has attracted efforts to repurpose it for novel functions, such as the synthesis of polymers containing non-natural building blocks. However, efforts to repurpose ribosomes are limited by the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7c91eedc82ea9d7be90cd3db7b07ec51
https://doi.org/10.1093/nar/gkaa001
https://doi.org/10.1093/nar/gkaa001
Autor:
Yan Zhang, Nikolay A. Aleksashin, Dorota Klepacki, Caleb Anderson, Nora Vázquez-Laslop, Carol A. Gross, Alexander S. Mankin
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America
Significance Several antibiotics targeting the large ribosomal subunit interfere with translation in a context-specific manner, preventing ribosomes from polymerizing specific amino acid sequences. Here, we reveal kasugamycin as a small ribosomal sub
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::189047a982f4c1d1f13bc9c1ab068b43
https://pubmed.ncbi.nlm.nih.gov/35064089
https://pubmed.ncbi.nlm.nih.gov/35064089
Autor:
Alexander S. Mankin, Jaanus Remme, Margus Leppik, Nora Vázquez-Laslop, Michael C. Jewett, Nikolay A. Aleksashin, Adam J. Hockenberry, Dorota Klepacki
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
Nature Communications
Nature Communications
Ribo-T is an engineered ribosome whose small and large subunits are tethered together by linking 16S rRNA and 23S rRNA in a single molecule. Although Ribo-T can support cell proliferation in the absence of wild type ribosomes, Ribo-T cells grow slowe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dddf6f6138577a4a0e32fb3c1b82e068
http://link.springer.com/article/10.1038/s41467-019-08892-w
http://link.springer.com/article/10.1038/s41467-019-08892-w
Autor:
Anna B. Loveland, Amira Kefi, Nora Vázquez-Laslop, Kaspar Reier, Shijie Huang, Teresa Szal, Jaanus Remme, Nikolay A. Aleksashin, Andrei A. Korostelev, Dorota Klepacki, Alexander S. Mankin, Luc Jaeger
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-13 (2020)
Nature Communications
Nature Communications
5S rRNA is an indispensable component of cytoplasmic ribosomes in all species. The functions of 5S rRNA and the reasons for its evolutionary preservation as an independent molecule remain unclear. Here we used ribosome engineering to investigate whet
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::19a29b7ff3dade09fd7d94c1924d52bb