Measuring Myeloperoxidase Activity as a Marker of Inflammation in Gut Tissue Samples of Mice and Rat

Autor: Nikita Hanning, Joris De Man, Benedicte De Winter

Publikováno v: Bio-Protocol, Vol 13, Iss 13 (2023)

Myeloperoxidase (MPO) is an enzyme contained in lysosomal azurophilic granules of neutrophils. MPO activity has been shown to correlate with the number of neutrophils in histological sections of the gastrointestinal tract and is therefore accepted as

Externí odkaz: https://doaj.org/article/e2c272929a424b25b31dbe12596d51f3

The Effect of Serine Protease Inhibitors on Visceral Pain in Different Rodent Models With an Intestinal Insult

Autor: Hannah Ceuleers, Nikita Hanning, Michelle De bruyn, Joris G De Man, Heiko U De Schepper, Qian Li, Liansheng Liu, Steven Abrams, Annemieke Smet, Jurgen Joossens, Koen Augustyns, Ingrid De Meester, Pankaj J Pasricha, Benedicte Y De Winter

Publikováno v: Frontiers in Pharmacology, Vol 13 (2022)

Background: Serine proteases are believed to play a key role in the origin of abdominal pain in IBD and IBS. We previously demonstrated a reduction of visceral pain in a post-inflammatory IBS rat model after a single intraperitoneal or intracolonic a

Externí odkaz: https://doaj.org/article/2e982d8ea77b4cd0af0421bfcbe928ff

Local Colonic Administration of a Serine Protease Inhibitor Improves Post-Inflammatory Visceral Hypersensitivity in Rats

Autor: Nikita Hanning, Michelle De bruyn, Hannah Ceuleers, Tim Boogaerts, Maya Berg, Annemieke Smet, Heiko U. De Schepper, Jurgen Joossens, Alexander L. N. van Nuijs, Joris G. De Man, Koen Augustyns, Ingrid De Meester, Benedicte Y. De Winter

Publikováno v: Pharmaceutics, Vol 13, Iss 6, p 811 (2021)

Dysregulation of the protease–antiprotease balance in the gastrointestinal tract has been suggested as a mechanism underlying visceral hypersensitivity in conditions such as inflammatory bowel disease (IBD) and irritable bowel syndrome (IBS). We ai

Externí odkaz: https://doaj.org/article/bfb9756fc6404ddbabce04e440fd583d

Proteolytic Cleavage of Bioactive Peptides and Protease-Activated Receptors in Acute and Post-Colitis

Autor: Ulf-Håkan Stenman, Hannu Koistinen, Paco Hulpiau, Cedric Hermans, Benedicte Y. De Winter, Nikita Hanning, Ingrid De Meester, Hannah Ceuleers, Maya Berg, Joris G. De Man, Anne-Marie Lambeir, Michelle De bruyn

Publikováno v: International Journal of Molecular Sciences, Vol 22, Iss 10711, p 10711 (2021)
International journal of molecular sciences
International Journal of Molecular Sciences
Volume 22
Issue 19

The protease activity in inflammatory bowel disease (IBD) and irritable bowel syndrome has been studied extensively using synthetic fluorogenic substrates targeting specific sets of proteases. We explored activities in colonic tissue from a 2,4,6-tri

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7721792eaab3bae8b68e82bd936b0633
https://www.mdpi.com/1422-0067/22/19/10711

Gut microbial β-glucuronidases regulate host luminal proteases and are depleted in irritable bowel syndrome

Autor: Adam L. Edwinson, Lu Yang, Stephanie Peters, Nikita Hanning, Patricio Jeraldo, Pratik Jagtap, Joshua B. Simpson, Tzu-Yi Yang, Praveen Kumar, Subina Mehta, Asha Nair, Margaret Breen-Lyles, Lakshmikanth Chikkamenahalli, Rondell P. Graham, Benedicte De Winter, Robin Patel, Surendra Dasari, Purna Kashyap, Timothy Griffin, Jun Chen, Gianrico Farrugia, Matthew R. Redinbo, Madhusudan Grover

Publikováno v: Nature microbiology

Intestinal proteases mediate digestion and immune signalling, while increased gut proteolytic activity disrupts the intestinal barrier and generates visceral hypersensitivity, which is common in irritable bowel syndrome (IBS). However, the mechanisms

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cb93d97665a21ab88de9dafb6045f7cd
https://pubmed.ncbi.nlm.nih.gov/35524133