Zobrazeno 1 - 10
of 16
pro vyhledávání: '"Niki S.C. Wong"'
Publikováno v:
PMC
The mTOR pathway is a conserved master regulator of translational activity that influences the fate of industrially relevant CHO cell cultures, yet its molecular mechanisms remain unclear. Interestingly, rapamycin specific inhibition of the mTOR path
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6bcd4d62b7208692428ffc0eb62bcd21
https://doi.org/10.1016/j.jbiotec.2014.01.024
https://doi.org/10.1016/j.jbiotec.2014.01.024
Autor:
Sze Wai Ng, Hsueh Lee Lim, Bernard Loo, Franck C. Courtes, Miranda G.S. Yap, Niki S.C. Wong, Leah A. Vardy, Geoffrey Koh, Joyce Lin, Dong-Yup Lee
Publikováno v:
Journal of Biotechnology. 167:215-224
We report the first investigation of translational efficiency on a global scale, also known as translatome, of a Chinese hamster ovary (CHO) DG44 cell line producing monoclonal antibodies (mAb). The translatome data was generated via combined use of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::604c9047185b91b74ecbb64c965da7b5
https://doi.org/10.1016/j.jbiotec.2013.07.010
https://doi.org/10.1016/j.jbiotec.2013.07.010
Publikováno v:
Rapid Communications in Mass Spectrometry. 25:1407-1412
The biopharmaceutical industry has been in pursuit of strategies which can isolate stable and high-producing cell lines. The whole cell mass spectrometry method by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) is a rapid and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ba5f18bff69716a79e6fc4a38fdb3d57
https://doi.org/10.1002/rcm.5011
https://doi.org/10.1002/rcm.5011
Publikováno v:
Biotechnology and Bioengineering. 107:516-528
One of the goals of recombinant glycoprotein production is to achieve consistent glycosylation. Although many studies have examined the changes in the glycosylation quality of recombinant protein with culture, very little has been done to examine the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e95ad8dabec51d9cd76ac9fb0e580335
https://doi.org/10.1002/bit.22828
https://doi.org/10.1002/bit.22828
Autor:
Alois Jungbauer, Niki S.C. Wong, Ming Wei Wu, Anne Tscheliessnig, Poh Choo Toh, Julita K. Grzeskowiak, Yih Yean Lee, Janet Chusainow
Publikováno v:
ELECTROPHORESIS. 31:1862-1872
For therapeutic antibody production Protein A chromatography is often replaced by non-affinity-based purification sequences, which are considered as more economical. 2-D DIGE was applied for evaluation of scale-up of non-affinity based process of a h
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9d254fcb822a0e30ab043bb3b4aa6a3f
https://doi.org/10.1002/elps.200900781
https://doi.org/10.1002/elps.200900781
Publikováno v:
Biotechnology and Bioengineering. 107:321-336
Controlling glycosylation of recombinant proteins produced by CHO cells is highly desired as it can be directed towards maintaining or increasing product quality. To further our understanding of the different factors influencing glycosylation, a glyc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::da21f7c760ad43d89930aee4929163e9
https://doi.org/10.1002/bit.22812
https://doi.org/10.1002/bit.22812
Autor:
Satty G. Reddy, Ying Swan Ho, William P. K. Chong, Chew-Kiat Heng, Miranda G.S. Yap, Niki S.C. Wong, Dong-Yup Lee, Faraaz N.K. Yusufi
Publikováno v:
Journal of Biotechnology. 147:116-121
We have established a liquid chromatography-mass spectrometry based metabolomics platform to identify extracellular metabolites in the medium of recombinant Chinese hamster ovary (CHO) fed-batch reactor cultures. Amongst the extracellular metabolites
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f24184eafcbb2e0e45b68cb74003ca06
https://doi.org/10.1016/j.jbiotec.2010.03.018
https://doi.org/10.1016/j.jbiotec.2010.03.018
Autor:
Satty G. Reddy, Lin Tang Goh, Chew-Kiat Heng, Ying Swan Ho, William P. K. Chong, Miranda G.S. Yap, Niki S.C. Wong, Faraaz N.K. Yusufi, Dong-Yup Lee
Publikováno v:
Rapid Communications in Mass Spectrometry. 23:3763-3771
A metabolomics-based approach was used to time profile extracellular metabolites in duplicate fed-batch bioreactor cultures of recombinant Chinese Hamster Ovary (CHO) cells producing monoclonal IgG antibody. Culture medium was collected and analysed
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b16b5ab8504b277c4719f33845b4d028
https://doi.org/10.1002/rcm.4328
https://doi.org/10.1002/rcm.4328
Autor:
Yih Yean Lee, Anne Tscheliessnig, Julita K. Grzeskowiak, Janet Chusainow, Poh Choo Toh, Alois Jungbauer, Niki S.C. Wong
Publikováno v:
Protein Expression and Purification. 66:58-65
Two-dimensional fluorescence difference gel electrophoresis (2-D DIGE) is an established method for assessing protein expression strategies, understanding pathogenesis mechanisms, characterizing biomarkers, and controlling therapeutic processes. We a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bb176188504aed09f24532392f3df9d7
https://doi.org/10.1016/j.pep.2009.01.007
https://doi.org/10.1016/j.pep.2009.01.007
Autor:
Poh Choo Toh, Yih Yean Lee, Niki S.C. Wong, Anne Tscheliessnig, Julita K. Grzeskowiak, Janet Chusainow, Alois Jungbauer
Publikováno v:
Journal of Chromatography A. 1216:4902-4912
Although Staphylococcus Protein A (SpA) affinity chromatography is the state of the art capture step for antibody purification, non-affinity methods are more economical. We used two-dimensional fluorescence difference gel electrophoresis (2-D DIGE) t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6d0ad49d5660910fa96821f8afc6876e
https://doi.org/10.1016/j.chroma.2009.04.014
https://doi.org/10.1016/j.chroma.2009.04.014