Zobrazeno 1 - 10
of 73
pro vyhledávání: '"Nigel J. Savery"'
ChipSeg: An Automatic Tool to Segment Bacterial and Mammalian Cells Cultured in Microfluidic Devices
Autor:
Irene de Cesare, Criseida G. Zamora-Chimal, Lorena Postiglione, Mahmoud Khazim, Elisa Pedone, Barbara Shannon, Gianfranco Fiore, Giansimone Perrino, Sara Napolitano, Diego di Bernardo, Nigel J. Savery, Claire Grierson, Mario di Bernardo, Lucia Marucci
Publikováno v:
ACS Omega, Vol 6, Iss 4, Pp 2473-2476 (2021)
Externí odkaz:
https://doaj.org/article/d14265a544524f38b27532d973d25878
Publikováno v:
eLife, Vol 10 (2021)
The PcrA/UvrD helicase binds directly to RNA polymerase (RNAP) but the structural basis for this interaction and its functional significance have remained unclear. In this work, we used biochemical assays and hydrogen-deuterium exchange coupled to ma
Externí odkaz:
https://doaj.org/article/db4351289616495e887dd60ee4c933e7
Autor:
Harry F. Thompson, Joseph L. Beesley, Hannah D. Langlands, Caitlin L. Edgell, Nigel J. Savery, Derek N. Woolfson
Publikováno v:
ACS Synthetic Biology. 12:1308-1319
Autor:
Abigail J. Smith, Elise A. Naudin, Caitlin L. Edgell, Emily G. Baker, Bram Mylemans, Laura FitzPatrick, Andrew Herman, Helen M. Rice, David M. Andrews, Natalie Tigue, Derek N. Woolfson, Nigel J. Savery
Publikováno v:
ACS Synthetic Biology.
Autor:
Guto G. Rhys, Jessica A. Cross, William M. Dawson, Harry F. Thompson, Sooruban Shanmugaratnam, Nigel J. Savery, Mark P. Dodding, Birte Höcker, Derek N. Woolfson
Publikováno v:
Rhys, G G, Cross, J A, Dawson, W M, Thompson, H F, Shanmugaratnam, S, Savery, N J, Dodding, M P, Höcker, B & Woolfson, D N 2022, ' De novo designed peptides for cellular delivery and subcellular localisation ', Nature Chemical Biology, vol. 18, no. 9, pp. 999-1004 . https://doi.org/10.1038/s41589-022-01076-6
Nature Chemical Biology
Nature Chemical Biology
Increasingly, it is possible to design peptide and protein assemblies de novo from first principles or computationally. This approach provides new routes to functional synthetic polypeptides, including designs to target and bind proteins of interest.
Autor:
Elise A. Naudin, Katherine I. Albanese, Abigail J. Smith, Bram Mylemans, Emily G. Baker, Orion D. Weiner, David M. Andrews, Natalie Tigue, Nigel J. Savery, Derek N. Woolfson
Publikováno v:
Naudin, E A, Albanese, K I, Smith, A J, Mylemans, B, Baker, E G, Savery, N J, Woolfson, D N & et, A 2022, ' From peptides to proteins : coiled-coil tetramers to single-chain 4-helix bundles ', Chemical Science, vol. 13, no. 38, pp. 11330-11340 . https://doi.org/10.1039/D2SC04479J
The design of completely synthetic proteins from first principles—de novo protein design—is challenging. This is because, despite recent advances in computational protein-structure prediction and design, we do not understand fully the sequence-to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::49de030d354e6b82f0d5fa2d6401d9fd
https://doi.org/10.1101/2022.08.04.502660
https://doi.org/10.1101/2022.08.04.502660
Publikováno v:
Edgell, C L, Savery, N J & Woolfson, D N 2020, ' Robust de novo designed homotetrameric coiled coils ', Biochemistry . https://doi.org/10.1021/acs.biochem.0c00082
De novo-designed protein domains are increasingly being applied in biotechnology, cell biology, and synthetic biology. Therefore, it is imperative that these proteins be robust to superficial changes; i.e., small changes to their amino acid sequences
Publikováno v:
Edgell, C L, Smith, A J, Beesley, J L, Savery, N J & Woolfson, D N 2020, ' De Novo Designed Protein-Interaction Modules for In-Cell Applications ', ACS Synthetic Biology, vol. 9, 2, pp. 427-436 . https://doi.org/10.1021/acssynbio.9b00453
Protein–protein interactions control a wide variety of natural biological processes. α-Helical coiled coils frequently mediate such protein–protein interactions. Due to the relative simplicity of their sequences and structures and the ease with
Autor:
Guto G, Rhys, Jessica A, Cross, William M, Dawson, Harry F, Thompson, Sooruban, Shanmugaratnam, Nigel J, Savery, Mark P, Dodding, Birte, Höcker, Derek N, Woolfson
Publikováno v:
Nature chemical biology. 18(9)
Increasingly, it is possible to design peptide and protein assemblies de novo from first principles or computationally. This approach provides new routes to functional synthetic polypeptides, including designs to target and bind proteins of interest.