Structural and biochemical characterization of the biuret hydrolase (BiuH) from the cyanuric acid catabolism pathway of Rhizobium leguminasorum bv. viciae 3841.

Autor: Lygie Esquirol, Thomas S Peat, Matthew Wilding, Del Lucent, Nigel G French, Carol J Hartley, Janet Newman, Colin Scott

Publikováno v: PLoS ONE, Vol 13, Iss 2, p e0192736 (2018)

Biuret deamination is an essential step in cyanuric acid mineralization. In the well-studied atrazine degrading bacterium Pseudomonas sp. strain ADP, the amidase AtzE catalyzes this step. However, Rhizobium leguminosarum bv. viciae 3841 uses an unrel

Externí odkaz: https://doaj.org/article/8bba02bef1104cd5a2e2240a88d92567

Engineered enzymes that retain and regenerate their cofactors enable continuous-flow biocatalysis

Autor: Greg Coia, Quentin I. Churches, Charlotte C. Williams, Andrew C. Warden, Nicholas J. Turner, Chantel N. Jensen, Greg Simpson, Andrew R. Frazer, Tom Nebl, Carol J. Hartley, Judith A. Scoble, Colin Scott, Nigel G. French, Andrea North

Publikováno v: Nature Catalysis. 2:1006-1015

Biocatalysis is an attractive route for the synthesis of complex organic molecules, such as pharmaceuticals, due to the properties of enzymes (high specificity and high catalytic rate). Ideally, we would be able to use enzymes in continuous-flow reac

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::68c0a4ff5bd4dceff0f1292f85f64687
https://doi.org/10.1038/s41929-019-0353-0

An unexpected vestigial protein complex reveals the evolutionary origins of an s-triazine catabolic enzyme

Autor: Christopher J. Easton, Thomas Nebl, Janet Newman, Hideki Onagi, Matthew Wilding, Nigel G. French, Thomas S. Peat, Jian-Wei Liu, Colin Scott, Lygie Esquirol, Carol J. Hartley

Publikováno v: Journal of Biological Chemistry. 293:7880-7891

Cyanuric acid is a metabolic intermediate of s-triazines, such as atrazine (a common herbicide) and melamine (used in resins and plastics). Cyanuric acid is mineralized to ammonia and carbon dioxide by the soil bacterium Pseudomonas sp. strain ADP vi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::181de71672391adb5cce668eca56a53d
https://doi.org/10.1074/jbc.ra118.001996

Sugar analog synthesis by in vitro biocatalytic cascade: A comparison of alternative enzyme complements for dihydroxyacetone phosphate production as a precursor to rare chiral sugar synthesis

Autor: Judith A. Scoble, Carol J. Hartley, Gregory W. Simpson, Colin Scott, Matthew C. Taylor, Quentin I. Churches, Andrew R. Frazer, Nicholas J. Turner, Greg Coia, Charlotte C. Williams, Nigel G. French

Publikováno v: PLoS ONE
Hartley, C J, French, N G, Scoble, J A, Williams, C C, Churches, Q I, Frazer, A R, Taylor, M C, Coia, G, Simpson, G, Turner, N J & Scott, C 2017, ' Sugar analog synthesis by in vitro biocatalytic cascade : A comparison of alternative enzyme complements for dihydroxyacetone phosphate production as a precursor to rare chiral sugar synthesis ', PLoS ONE, vol. 12, no. 11, e0184183 . https://doi.org/10.1371/journal.pone.0184183
PLoS ONE, Vol 12, Iss 11, p e0184183 (2017)

Carbon-carbon bond formation is one of the most challenging reactions in synthetic organic chemistry, and aldol reactions catalysed by dihydroxyacetone phosphate-dependent aldolases provide a powerful biocatalytic tool for combining C-C bond formatio

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fb212bec50738b15d40a6fe9cef51db1
http://europepmc.org/articles/PMC5675407

Cyanuric acid hydrolase: evolutionary innovation by structural concatenation

Autor: Sahil Balotra, Santosh Panjikar, Colin Scott, Janet Newman, Thomas S. Peat, Matthew Wilding, Nathan Cowieson, Nigel G. French, Lyndall J. Briggs

Publikováno v: Molecular Microbiology

Summary The cyanuric acid hydrolase, AtzD, is the founding member of a newly identified family of ring-opening amidases. We report the first X-ray structure for this family, which is a novel fold (termed the ‘Toblerone’ fold) that likely evolved

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::51d5ca0dffee0cf35961f58f8731d31f
http://europepmc.org/articles/PMC3758960