Zobrazeno 1 - 10
of 30
pro vyhledávání: '"Niels Erik Møllegaard"'
Publikováno v:
PLoS ONE, Vol 9, Iss 3, p e91138 (2014)
Most protein purification procedures include an affinity tag fused to either the N or C-terminal end of the protein of interest as well as a procedure for tag removal. Tag removal is not straightforward and especially tag removal from the C-terminal
Externí odkaz:
https://doaj.org/article/54aa6160cde24412b38ae648c69d0f9a
Autor:
Morten F Gjerstorff, Heike I Rösner, Christina B Pedersen, Katrine B V Greve, Steffen Schmidt, Katherine L Wilson, Jan Mollenhauer, Hüseyin Besir, Flemming M Poulsen, Niels Erik Møllegaard, Henrik J Ditzel
Publikováno v:
PLoS ONE, Vol 7, Iss 9, p e45819 (2012)
GAGE proteins are highly similar, primate-specific molecules with unique primary structure and undefined cellular roles. They are restricted to cells of the germ line in adult healthy individuals, but are broadly expressed in a wide range of cancers.
Externí odkaz:
https://doaj.org/article/caa21b9d79494282bf95567b6de2f1c9
Autor:
Michele Mariotti, Søren Lindemose, Per Hägglund, Niels Erik Møllegaard, Fabian Leinisch, Michael J. Davies, Sofie Hagel Andersen
Publikováno v:
Leinisch, F, Mariotti, M, Andersen, S H, Lindemose, S, Hägglund, P, Møllegaard, N E & Davies, M J 2020, ' UV oxidation of cyclic AMP receptor protein, a global bacterial gene regulator, decreases DNA binding and cleaves DNA at specific sites ', Scientific Reports, vol. 10, no. 1, 3106 . https://doi.org/10.1038/s41598-020-59855-x
Scientific Reports
Scientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
Scientific Reports
Scientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
UV light is a widely-employed, and environmentally-sensitive bactericide but its mechanism of action is not fully defined. Proteins are major chromophores and targets for damage due to their abundance, but the role of proteins in inducing damage to b
Autor:
Rasmus Linde Bang Elnegaard, Qiang Zhang, Niels Erik Møllegaard, Frank Kjeldsen, Thomas J. D. Jørgensen
Publikováno v:
Elnegaard, R L B, Møllegaard, N E, Zhang, Q, Kjeldsen, F & Jørgensen, T J D 2017, ' Uranyl Photocleavage of Phosphopeptides Yields Truncated C-Terminally Amidated Peptide Products ', ChemBioChem, vol. 18, no. 12, pp. 1117-1122 . https://doi.org/10.1002/cbic.201700103
Chembiochem
ChemBioChem
Chembiochem
ChemBioChem
The uranyl ion (UO2(2+) ) binds phosphopeptides with high affinity, and when irradiated with UV-light, it can cleave the peptide backbone. In this study, high-accuracy tandem mass spectrometry and enzymatic assays were used to characterise the photoc
Autor:
Nadine Heidi Brückmann, Morten F. Gjerstorff, Sofie Traynor, Henrik J. Ditzel, Simone Johansen, Jérôme Déjardin, Niels Erik Møllegaard, Mikkel G. Terp, Christina Bøg Pedersen, Mikkel Girke Jørgensen
Publikováno v:
Nucleic Acids Research
Traynor, S, Møllegaard, N E, Jørgensen, M G, Brückmann, N H, Pedersen, C B, Terp, M G, Johansen, S, Dejardin, J, Ditzel, H J & Gjerstorff, M F 2019, ' Remodeling and destabilization of chromosome 1 pericentromeric heterochromatin by SSX proteins ', Nucleic Acids Research, vol. 47, no. 13, pp. 6668-6684 . https://doi.org/10.1093/nar/gkz396
Traynor, S, Møllegaard, N E, Jørgensen, M G, Brückmann, N H, Pedersen, C B, Terp, M G, Johansen, S, Dejardin, J, Ditzel, H J & Gjerstorff, M F 2019, ' Remodeling and destabilization of chromosome 1 pericentromeric heterochromatin by SSX proteins ', Nucleic acids research, vol. 47, no. 13, pp. 6668-6684 . https://doi.org/10.1093/nar/gkz396
Traynor, S, Møllegaard, N E, Jørgensen, M G, Brückmann, N H, Pedersen, C B, Terp, M G, Johansen, S, Dejardin, J, Ditzel, H J & Gjerstorff, M F 2019, ' Remodeling and destabilization of chromosome 1 pericentromeric heterochromatin by SSX proteins ', Nucleic Acids Research, vol. 47, no. 13, pp. 6668-6684 . https://doi.org/10.1093/nar/gkz396
Traynor, S, Møllegaard, N E, Jørgensen, M G, Brückmann, N H, Pedersen, C B, Terp, M G, Johansen, S, Dejardin, J, Ditzel, H J & Gjerstorff, M F 2019, ' Remodeling and destabilization of chromosome 1 pericentromeric heterochromatin by SSX proteins ', Nucleic acids research, vol. 47, no. 13, pp. 6668-6684 . https://doi.org/10.1093/nar/gkz396
Rearrangement of the 1q12 pericentromeric heterochromatin and subsequent amplification of the 1q arm is commonly associated with cancer development and progression and may result from epigenetic deregulation. In many premalignant and malignant cells,
Autor:
Per L. Gregersen, Dagmara Podzimska-Sroka, Charlotte O'Shea, Michael W. Christiansen, Kim H. Hebelstrup, Colette Matthewman, Karen Skriver, Niels Erik Møllegaard, Søren Lindemose, Inger Bæksted Holme
Publikováno v:
Journal of Experimental Botany
Christiansen, M W, Matthewman, C, Podzimska-Sroka, D, O'Shea, C, Lindemose, S, Møllegaard, N E, Holme, I B, Hebelstrup, K, Skriver, K & Gregersen, P L 2016, ' Barley plants over-expressing the NAC transcription factor gene HvNAC005 show stunting and delay in development combined with early senescence ', Journal of Experimental Botany, vol. 67, no. 17, pp. 5259-5273 . https://doi.org/10.1093/jxb/erw286
Christiansen, M W, Matthewman, C, Podzimska-Sroka, D, O'Shea, C, Lindemose, S, Møllegaard, N E, Holme, I B, Hebelstrup, K, Skriver, K & Gregersen, P L 2016, ' Barley plants over-expressing the NAC transcription factor gene HvNAC005 show stunting and delay in development combined with early senescence ', Journal of Experimental Botany, vol. 67, no. 17, pp. 5259-5273 . https://doi.org/10.1093/jxb/erw286
Highlight HvNAC005 was shown to be a strong positive regulator of senescence, involved in regulation in the cross field of different hormone and signalling pathways controlling developmental senescence in barley.
The plant-specific NAC transcrip
The plant-specific NAC transcrip
Publikováno v:
Nucleic Acids Research
The double-stranded DNA of the genome contains both sequence information directly relating to the protein and RNA coding as well as functional and structural information relating to protein recognition. Only recently is the importance of DNA shape in
Autor:
Peter E. Nielsen, Birthe B. Kragelund, Niels Erik Møllegaard, Line H. Kristensen, Christian Isak Jørgensen
Publikováno v:
ChemBioChem. 9:2377-2381
Publikováno v:
Nucleic Acids Research
The DNA interaction of the Escherichia coli cyclic AMP receptor protein (CRP) represents a typical example of a dual recognition mechanism exhibiting both direct and indirect readout. We have dissected the direct and indirect components of DNA recogn
Publikováno v:
Biochemistry. 44:7855-7863
In this study, we have systematically compared the uranyl photocleavage of a range of bent A-tracts and nonbent TA-tracts as well as interrupted A-tracts. We demonstrate that uranyl photocleavage of A-tracts and TA-tractsis almost identical, indicati