Zobrazeno 1 - 10
of 78
pro vyhledávání: '"Niels, Geijsen"'
Autor:
Clara Martínez Mir, Paola Pisterzi, Isabel De Poorter, Maria Rilou, Melissa van Kranenburg, Bram Heijs, Anna Alemany, Fanny Sage, Niels Geijsen
Publikováno v:
Communications Biology, Vol 7, Iss 1, Pp 1-16 (2024)
Abstract Myofibers are large multinucleated cells that have long thought to have a rather simple organization. Single-nucleus transcriptomics, spatial transcriptomics and spatial metabolomics analysis have revealed distinct transcription profiles in
Externí odkaz:
https://doaj.org/article/afeade6f482741f19de43bcbc0c2a250
Autor:
Paola Pisterzi, Clara Martinez Mir, Ouafa Dahri, Isabel de Poorter, Sandra Batlles Parera, Milica Dostanić, Massimo Mastrangeli, Christine Mummery, Niels Geijsen, Fanny Sage
Publikováno v:
STAR Protocols, Vol 5, Iss 4, Pp 103378- (2024)
Summary: Skeletal muscle spatial analyses have revealed unexpected regionalized gene expression patterns challenging the understanding of muscle as a homogeneous tissue. Here, we present a protocol for the spatial analysis of transcript and protein l
Externí odkaz:
https://doaj.org/article/1ee48d89adb94b6b87659533164f4bf4
Autor:
Albert Blanch-Asensio, Babet van der Vaart, Mariana Vinagre, Eline Groen, Christiaan Arendzen, Christian Freund, Niels Geijsen, Christine L. Mummery, Richard P. Davis
Publikováno v:
Stem Cell Research, Vol 66, Iss , Pp 102991- (2023)
STRAIGHT-IN is a platform to precisely integrate DNA payloads into the genome of cells, including hiPSCs. Here, we generated two hiPSC acceptor lines each with one copy of an upgraded landing pad (LP). This improved design allows more efficient (∼1
Externí odkaz:
https://doaj.org/article/1226a2ec50804aaa818af1245a0a9948
Autor:
Bas Castelijns, Mirna L. Baak, Ilia S. Timpanaro, Caroline R. M. Wiggers, Marit W. Vermunt, Peng Shang, Ivanela Kondova, Geert Geeven, Valerio Bianchi, Wouter de Laat, Niels Geijsen, Menno P. Creyghton
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-12 (2020)
The understanding of the changes regulating gene expression relevant for the emergence of the human brain and its susceptibility to disease is limited. Here, the authors identified a set of regulatory elements that evolved in hominins affecting oligo
Externí odkaz:
https://doaj.org/article/82266434e4204259b6a7fa449dc212bd
Autor:
Ator Ashoti, Francesco Limone, Melissa van Kranenburg, Anna Alemany, Mirna Baak, Judith Vivié, Frederica Piccioni, Pascale F. Dijkers, Menno Creyghton, Kevin Eggan, Niels Geijsen
Publikováno v:
PLoS ONE, Vol 17, Iss 2 (2022)
Genome-wide screens that have viability as a readout have been instrumental to identify essential genes. The development of gene knockout screens with the use of CRISPR-Cas has provided a more sensitive method to identify these genes. Here, we perfor
Externí odkaz:
https://doaj.org/article/7a34dd889a6644cbac3dc84f3a0a657a
Autor:
Melanie Krüger, Alicia Ruppelt, Benjamin Kappler, Elke Van Soest, Roos Anne Samsom, Guy C. M. Grinwis, Niels Geijsen, J. Bernd Helms, Marco Stijnen, Linda M. Kock, Marco Rasponi, Hans S. Kooistra, Bart Spee
Publikováno v:
Bioengineering, Vol 9, Iss 9, p 471 (2022)
Metabolic and toxic liver disorders, such as fatty liver disease (steatosis) and drug-induced liver injury, are highly prevalent and potentially life-threatening. To allow for the study of these disorders from the early stages onward, without using e
Externí odkaz:
https://doaj.org/article/6cef8376ae704fadbfe270662ee227a7
Autor:
Hedwig S. Kruitwagen, Loes A. Oosterhoff, Ingrid G.W.H. Vernooij, Ingrid M. Schrall, Monique E. van Wolferen, Farah Bannink, Camille Roesch, Lisa van Uden, Martijn R. Molenaar, J. Bernd Helms, Guy C.M. Grinwis, Monique M.A. Verstegen, Luc J.W. van der Laan, Meritxell Huch, Niels Geijsen, Robert G. Vries, Hans Clevers, Jan Rothuizen, Baukje A. Schotanus, Louis C. Penning, Bart Spee
Publikováno v:
Stem Cell Reports, Vol 8, Iss 4, Pp 822-830 (2017)
Summary: Hepatic steatosis is a highly prevalent liver disease, yet research is hampered by the lack of tractable cellular and animal models. Steatosis also occurs in cats, where it can cause severe hepatic failure. Previous studies demonstrate the p
Externí odkaz:
https://doaj.org/article/1b75311bbf3843328b88a26d9590e37a
Autor:
Paul A. De Sousa, Rachel Steeg, Elisabeth Wachter, Kevin Bruce, Jason King, Marieke Hoeve, Shalinee Khadun, George McConnachie, Julie Holder, Andreas Kurtz, Stefanie Seltmann, Johannes Dewender, Sascha Reimann, Glyn Stacey, Orla O'Shea, Charlotte Chapman, Lyn Healy, Heiko Zimmermann, Bryan Bolton, Trisha Rawat, Isobel Atkin, Anna Veiga, Bernd Kuebler, Blanca Miranda Serano, Tomo Saric, Jürgen Hescheler, Oliver Brüstle, Michael Peitz, Cornelia Thiele, Niels Geijsen, Bjørn Holst, Christian Clausen, Majlinda Lako, Lyle Armstrong, Shailesh K. Gupta, Alexander J. Kvist, Ryan Hicks, Anna Jonebring, Gabriella Brolén, Andreas Ebneth, Alfredo Cabrera-Socorro, Patrik Foerch, Martine Geraerts, Tina C. Stummann, Shawn Harmon, Carol George, Ian Streeter, Laura Clarke, Helen Parkinson, Peter W. Harrison, Adam Faulconbridge, Luca Cherubin, Tony Burdett, Cesar Trigueros, Minal J Patel, Christa Lucas, Barry Hardy, Rok Predan, Joh Dokler, Maja Brajnik, Oliver Keminer, Ole Pless, Philip Gribbon, Carsten Claussen, Annette Ringwald, Beate Kreisel, Aidan Courtney, Timothy E. Allsopp
Publikováno v:
Stem Cell Research, Vol 20, Iss C, Pp 105-114 (2017)
A fast track “Hot Start” process was implemented to launch the European Bank for Induced Pluripotent Stem Cells (EBiSC) to provide early release of a range of established control and disease linked human induced pluripotent stem cell (hiPSC) line
Externí odkaz:
https://doaj.org/article/d5c03414c1b04475bf195ed59dedab4f
Publikováno v:
Trends in Biotechnology.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4151fb9ae26b9817162b73b46815315b
https://doi.org/10.1016/j.tibtech.2023.03.004
https://doi.org/10.1016/j.tibtech.2023.03.004
Autor:
Gerardo Cedillo-Servin, Ouafa Dahri, João Meneses, Joost van Duijn, Fanny Sage, Joana Silva, André Pereira, Fernão D. Magalhães, Jos Malda, Niels Geijsen, Artur M. Pinto, Miguel Castilho
Tunable culture platforms that guide cellular organization and mechanically stimulate skeletal muscle development are still unavailable due to limitations in biocompatibility and actuation triggered without contact. This study reports the rational de
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::426c7042080ac61e64b045cf6dd333fb
https://doi.org/10.1101/2023.01.19.524679
https://doi.org/10.1101/2023.01.19.524679
