Zobrazeno 1 - 10
of 22
pro vyhledávání: '"Nicolette Pegels"'
Publikováno v:
LWT - Food Science and Technology. 60:867-875
This work reports four TaqMan real-time PCR assays for authenticity testing of wheat, barley, rye and oat species in food and feed. The method uses three specific primer/probe systems targeting DNA fragments from the ITS1 region for the detection of
Publikováno v:
Food Analytical Methods. 8:489-498
Food-labelling regulations require that meat species in food and feed are accurately declared to the consumer. Traceability systems based on species identification through DNA analysis are potent tools for the supervision of food adulteration. In thi
Autor:
Inés López-Calleja, Isabel González, Rosario Martín, Teófilo Zamarreño García, Nicolette Pegels
Publikováno v:
Food Additives & Contaminants: Part A. 30:771-779
Food and feed traceability has become a priority for governments due to consumer demand for comprehensive and integrated safety policies. In the present work, a TaqMan real-time PCR assay targeting the mitochondrial 12S rRNA gene was developed for sp
Autor:
Isabel González, Inés López-Calleja, Teresa García, Rosario Martín, Nicolette Pegels, Silvia de la Cruz
Publikováno v:
Food Control. 30:480-490
Peanut allergic reactions can result from the ingestion of even very small quantities of peanut and represent a severe threat to the health of sensitized individuals. In order to protect the allergic consumer, efficient and reliable methods are requi
Publikováno v:
Food Analytical Methods. 6:1040-1048
Animal species identification in food and feed has gained increasing interest in recent years due to public health, economic and legal concerns. In the present work, a TaqMan real-time PCR assay targeting the mitochondrial 12S rRNA gene was developed
Publikováno v:
Food Additives & Contaminants: Part A. 29:1402-1412
A TaqMan real-time PCR method was developed for specific detection of porcine-prohibited material in industrial feeds. The assay combines the use of a porcine-specific primer pair, which amplifies a 79 bp fragment of the mitochondrial (mt) 12 S rRNA
Autor:
Nicolette Pegels, Isabel González, Inés López-Calleja, S. Fernández, Rosario Martín, Teófilo Zamarreño García
Publikováno v:
Poultry Science. 91:1709-1719
A TaqMan real-time PCR method based on nucleotide sequence variation in the D-loop and 12S rRNA mitochondrial genes has been developed for the specific detection of chicken, turkey, duck, and goose prohibited material in animal feeds. The assay uses
Detection of Banned Ruminant-Derived Material in Industrial Feedstuffs by TaqMan Real-Time PCR Assay
Publikováno v:
Journal of Food Protection. 74:1300-1308
A ruminant-specific real-time PCR system was designed and applied for the detection of processed animal protein from ruminants in industrial feedstuffs. The assay includes a primer pair and a TaqMan probe selectively targeting mitochondrial 16S rRNA
Autor:
María Rojas, Isabel González, Nicolette Pegels, Rosario Martín, Irene Martínez Martín, Teresa García
Publikováno v:
Food Control. 22:1189-1196
A TaqMan real-time polymerase chain reaction (PCR) method was developed for specific detection of bovine, ovine and caprine processed animal protein (PAP) in industrial feedstuffs. The method uses species-specific primers and probes targeting short m
Autor:
Miguel Ángel Pavón, Teresa García, Isabel González, María Rojas, Nicolette Pegels, Pablo E. Hernández, Rosario Martín
Publikováno v:
Forensic Science International. 210:133-138
A rapid and highly species-specific real-time polymerase chain reaction (PCR) assay has been developed for the detection of capercaillie DNA (Tetrao urogallus) in meat and meat mixtures. The method combines the use of capercaillie-specific primers, t