Zobrazeno 1 - 5
of 5
pro vyhledávání: '"Nicolette D. Halloran"'
Autor:
Nicolette D. Halloran, Jean Thierry-Mieg, T. Hawkins, Philip Green, Mary Berks, Charles E. Martin, C. Huynh, Zijin Du, Mark M. Metzstein, K. Thomas, M. Craxton, Alan Coulson, LaDeana W. Hillier, Robert H. Waterston, John Sulston, Richard Durbin, Richard K. Wilson, Ratna Shownkeen
Publikováno v:
Nature Genetics. 1:114-123
As an adjunct to the genomic sequencing of Caenorhabditis elegans, we have investigated a representative cDNA library of 1,517 clones. A single sequence read has been obtained from the 5' end of each clone, allowing its characterization with respect
Publikováno v:
Electrophoresis. 13(8)
A major limitation in the applicability of automated DNA sequencing instruments has been the difficulty in using user-defined oligonucleotide primers which allow sequencing reactions to start at any specific point in a region of interest. Recently, n
Autor:
Charles R. Connell, Nicolette D. Halloran, Richard D. Cheng, Linda G. Lee, Carl W. Fuller, Rick K. Wilson, Sam Lee Woo, Bernard F. McArdle
Publikováno v:
Nucleic acids research. 20(10)
The incorporation of fluorescently labeled dideoxynucleotides by T7 DNA polymerase is optimized by the use of Mn2+, fluorescein analogs and four 2'-deoxyribonucleoside 5'-O-(1-thiotriphosphates) (dNTP alpha S's). The one-tube extension protocol was t
Autor:
Rodger Staden, Alan Coulson, K. Thomas, Jean Thierry-Mieg, Philip Green, Mark M. Metzstein, T. Hawkins, S. Dear, L. Qiu, M. Berks, R. Ainscough, Robert H. Waterston, LaDeana W. Hillier, Richard K. Wilson, Nicolette D. Halloran, R. Durbin, John Sulston, Zijin Du, M. Craxton
Publikováno v:
Nature. 356(6364)
The long-term goal of this project is the elucidation of the complete sequence of the Caenorhabditis elegans genome. During the first year methods have been developed and a strategy implemented that is amenable to large-scale sequencing. The three co