Sterol Derivatives Specifically Increase Anti-Inflammatory Oxylipin Formation in M2-like Macrophages by LXR-Mediated Induction of 15-LOX

Autor: Reiichi Ohno, Malwina Mainka, Rebecca Kirchhoff, Nicole M. Hartung, Nils Helge Schebb

Publikováno v: Molecules, Vol 29, Iss 8, p 1745 (2024)

The understanding of the role of LXR in the regulation of macrophages during inflammation is emerging. Here, we show that LXR agonist T09 specifically increases 15-LOX abundance in primary human M2 macrophages. In time- and dose-dependent incubations

Externí odkaz: https://doaj.org/article/9aee0a11ea6746d79745d2263d794c39

Development of a quantitative proteomics approach for cyclooxygenases and lipoxygenases in parallel to quantitative oxylipin analysis allowing the comprehensive investigation of the arachidonic acid cascade

Autor: Nicole M. Hartung, Malwina Mainka, Rebecca Pfaff, Michael Kuhn, Sebastian Biernacki, Lilli Zinnert, Nils Helge Schebb

Publikováno v: Analytical and Bioanalytical Chemistry. 415:913-933

Oxylipins derived from the cyclooxygenase (COX) and lipoxygenase (LOX) pathways of the arachidonic acid (ARA) cascade are essential for the regulation of the inflammatory response and many other physiological functions. Comprehensive analytical metho

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::e87f0badd5f9d7b6f9ba2adf238ceaeb
https://doi.org/10.1007/s00216-022-04489-3

Simple Targeted Assays for Metabolic Pathways and Signaling: A Powerful Tool for Targeted Proteomics

Autor: Bo Burla, Douglas G. Mashek, Dominik Kopczynski, Jan Medenbach, Michael R. Kreutz, René P. Zahedi, Federico Torta, Hans Frieder Schött, Albert Sickmann, Robert Ahrends, Cristina Coman, Andreas Hentschel, Nils Helge Schebb, Thorsten Hornemann, Kristina Lorenz, Nicole M. Hartung, Olga Shevchuk, Christer S. Ejsing

Publikováno v: Kopczynski, D, Hentschel, A, Coman, C, Schebb, N H, Hornemann, T, Mashek, D G, Hartung, N M, Shevchuk, O, Schött, H F, Lorenz, K, Torta, F, Burla, B, Zahedi, R P, Sickmann, A, Kreutz, M R, Ejsing, C S, Medenbach, J & Ahrends, R 2020, ' Simple Targeted Assays for Metabolic Pathways and Signaling : A Powerful Tool for Targeted Proteomics ', Analytical chemistry, vol. 92, no. 20, pp. 13672-13676 . https://doi.org/10.1021/acs.analchem.0c02793
Analytical Chemistry
Analytical chemistry 92(20), 13672-13676 (2020). doi:10.1021/acs.analchem.0c02793

We introduce STAMPS, a pathway-centric web service for the development of targeted proteomics assays. STAMPS guides the user by providing several intuitive interfaces for a rapid and simplified method design. Applying our curated framework to signali

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5bb33b87c8295f6eacd4b3e6aa0d2031
https://doi.org/10.1021/acs.analchem.0c02793

Untersuchung der Wirkung von Polyphenolen auf Cyclooxygenasen und Lipoxygenasen mittels targeted Proteomics und Metabolomics Methoden

Autor: Nicole M. Hartung, Nils Helge Schebb

Publikováno v: Lebensmittelchemie. 75

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::f74c3eae2789dead4c8ccef8040472ca
https://doi.org/10.1002/lemi.202158005

Combined Targeted Proteomics and Oxylipin Metabolomics for Monitoring of the COX-2 Pathway

Autor: Stephan Immenschuh, Nils Helge Schebb, Nicole M. Hartung, Annika I. Ostermann

Publikováno v: Proteomics. 21(3-4)

The important role of inducible cyclooxygenase-2 (COX-2) in several diseases necessitates analytical tools enabling thorough understanding of its modulation. Analysis of a comprehensive oxylipin pattern provides detailed information about changes in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::81062563cf2f7a6d75fa6defa5bc21c4
https://pubmed.ncbi.nlm.nih.gov/32875715