A Novel Fusion of IL-10 Engineered to Traffic across Intestinal Epithelium to Treat Colitis

Autor: Radhika C. Desai, Hyo Jin Kim, Julia D. Mackay, Weijun Feng, Randall J. Mrsny, Lisa Song, Linh P. Nyugen, Nicole C. Fay, Mahmood Tahir, Sally Postlethwaite, Apurva Chandalia, Baby Periyanayaki Muthusamy, Keyi Liu, Michael P. Coyle, Olson Charles, Elbert Seto, Tom Hunter, Khushdeep Mangat, Alistair Taverner, Minji Seung, Aatif Alam, Maziyar Saberi

Publikováno v: J Immunol

IL-10 is a potent anti-inflammatory cytokine capable of suppressing a number of proinflammatory signals associated with intestinal inflammatory diseases, such as ulcerative colitis and Crohn's disease. Clinical use of human IL-10 (hIL-10) has been li

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bcacdc98e95024eef8eb40f1c5cc0b2a
https://doi.org/10.4049/jimmunol.2000848

Early T cell receptor signals globally modulate ligand:receptor affinities during antigen discrimination

Autor: Nicole C. Fay, Shalini T. Low-Nam, Geoff P. O’Donoghue, Rafal M. Pielak, Jenny J. Lin, Jay T. Groves, Katherine N. Alfieri

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America
Proceedings of the National Academy of Sciences of the United States of America, vol 114, iss 46

Significance Antigen discrimination by T cells is based on subtle differences in binding of the T cell receptor (TCR) for its peptide major histocompatibility complex (pMHC) ligand. While such binding characteristics are readily mapped with great pre

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9e663e557ce350908ed09021d4e7e129
https://pubmed.ncbi.nlm.nih.gov/29087297

Cellular binding, motion, and internalization of synthetic gene delivery polymers

Autor: Gaelen T. Hess, William H. Humphries, Nicole C. Fay, Christine K. Payne

Publikováno v: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1773:1583-1588

Using fluorescence microscopy we have tracked the cellular binding, surface motion, and internalization of polyarginine and polyethylenimine, cationic ligands used for gene and protein delivery. Each ligand was complexed with a quantum dot to provide

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c68fcf0c000b90d69cfd4a1d53eb2a5c
https://doi.org/10.1016/j.bbamcr.2007.07.009

The Effect of Costimulation on T Cell Receptor Dynamics

Autor: Katherine N. Alfieri, Jay T. Groves, Nicole C. Fay

Publikováno v: Biophysical Journal. 104:119a

T cells are able to robustly discern extremely low numbers of agonist peptide amidst a myriad of background peptides. Costimulation of CD28 on the T cell by CD80 on the antigen presenting cell is known to enhance the sensitivity of this recognition p

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::269f3bc3bbf24ee4851739619560b111
https://doi.org/10.1016/j.bpj.2012.11.686

SNAP-tag Fusion Proteins as a Platform for Studying T Cell Costimulation

Autor: Nicole C. Fay, Jay T. Groves, Katherine N. Alfieri

Publikováno v: Biophysical Journal. (2):119a

The costimulatory molecules CD80 and CD86 regulate T cell signaling through their interactions with CD28 on the T cell surface. Reports indicate that costimulation increases T cell activation and lowers the TCR triggering threshold. The regulatory ro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7f27014074f9f76fd5e0b859eae97aab