Managing Multi-center Flow Cytometry Data for Immune Monitoring

Autor: Scott White, Karoline Laske, Marij J.P. Welters, Nicole Bidmon, Sjoerd H. van der Burg, Cedrik M. Britten, Jennifer Enzor, Janet Staats, Kent J. Weinhold, Cécile Gouttefangeas, Cliburn Chan

Publikováno v: Cancer Informatics, Vol 2014, Iss Suppl. 7, Pp 111-122 (2015)

Externí odkaz: https://doaj.org/article/f6543352a9474985940a339021777607

BNT162b2 vaccine induces neutralizing antibodies and poly-specific T cells in humans

Autor: John L. Perez, Isabel Vogler, Evelyna Derhovanessian, Gábor Boros, David A. Cooper, Camila R. Fontes-Garfias, Kristen E. Pascal, Armin Schultz, Alexander Muik, Martin Bexon, Pei Yong Shi, Peter Koch, Ann Kathrin Eller, Verena Lörks, Mark Cutler, Daniel Maurus, Ludwig Heesen, Philip R. Dormitzer, Ugur Sahin, Kathrin U. Jansen, Manuel Tonigold, Jan Grützner, Azita J. Mahiny, Corinna Rosenbaum, Stefanie Bolte, Mathias Vormehr, Marie Cristine Kühnle, Sybille Baumann, Asaf Poran, Alexander Ulges, Alexandra Kemmer-Brück, Christos A. Kyratsous, Dirk Becker, Özlem Türeci, Alina Baum, Sebastian Brachtendorf, Lena M. Kranz, Carsten Boesler, Rolf Hilker, Tania Palanche, Julian Sikorski, Nicole Bidmon, Ulrich Luxemburger, David J. Langer, Jesse Z. Dong, Gábor Szabó, Jasmin Quandt, Katalin Karikó, Jonas Reinholz

Publikováno v: Nature. 595:572-577

BNT162b2, a lipid nanoparticle (LNP) formulated nucleoside-modified messenger RNA (mRNA) that encodes the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) spike glycoprotein (S) stabilized in the prefusion conformation, has demonstrated 9

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::a0154147b441b18af735efb00657c62f
https://doi.org/10.1038/s41586-021-03653-6

Development of an RNA-based kit for easy generation of TCR-engineered lymphocytes to control T-cell assay performance

Autor: Cedrik M. Britten, Gerty Schreibelt, Nicole Bidmon, Sonja Kind, Richard Rae, Cécile Gouttefangeas, Sine Reker Hadrup, Marij J. P. Welters, Deborah Joseph-Pietras, Karoline Laske, Dominik Maurer, Sjoerd H. van der Burg, Ugur Sahin

Publikováno v: Journal of Immunological Methods, 458, pp. 74-82
Journal of Immunological Methods, 458, 74-82
Journal of Immunological Methods

Cell-based assays to monitor antigen-specific T-cell responses are characterized by their high complexity and should be conducted under controlled conditions to lower multiple possible sources of assay variation. However, the lack of standard reagent

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3e317d53c5948438da543b72327deaeb
https://pubmed.ncbi.nlm.nih.gov/29684430

Harmonisation of short-term in vitro culture for the expansion of antigen-specific CD8(+) T cells with detection by ELISPOT and HLA-multimer staining

Autor: Cedrik M. Britten, Linda F. M. Stynenbosch, Christian H. Ottensmeier, Sjoerd H. van der Burg, Cécile Gouttefangeas, Marij J. P. Welters, H. C. Schröder, Lindsey Chudley, Angelica Cazaly, Pedro Romero, Karoline Laske, Nicole Bidmon, Steffen Walter, Katy J. McCann, Dominik Maurer, Adam Coleman, Camilla Jandus

Publikováno v: Cancer Immunology, Immunotherapy, 63(11), 1199-1211
Cancer Immunology, Immunotherapy
Cancer Immunology, Immunotherapy : Cii, vol. 63, no. 11, pp. 1199-211

Ex vivo ELISPOT and multimer staining are well-established tests for the assessment of antigen-specific T cells. Many laboratories are now using a period of in vitro stimulation (IVS) to enhance detection. Here, we report the findings of a multi-cent

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a1e596f04fa296f70a859aa75063d3d9
http://hdl.handle.net/1887/104594

Serum-free freezing media support high cell quality and excellent ELISPOT assay performance across a wide variety of different assay protocols

Autor: Nicole Bidmon, Sebastian Attig, Sjoerd H. van der Burg, Cedrik M. Britten, Marij J. P. Welters, Helene Filbert, Ugur Sahin, Cécile Gouttefangeas, Sylvia Janetzki, Christian H. Ottensmeier, Bernhard Y. Renard

Publikováno v: Cancer Immunology, Immunotherapy
Cancer Immunology, Immunotherapy, 62(4), 615-627

Robust and sensitive ELISPOT protocols are commonly applied concomitant with the development of new immunotherapeutics. Despite the knowledge that individual serum batches differ in their composition and may change properties over time, serum is stil

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::abaacfd1fe5a96368aacf9bec326b2a4
https://hdl.handle.net/1887/96784