Protocol for capturing trophectoderm stem cells reflecting the blastocyst stage

Autor: Jinwoo Seong, Nicolas C. Rivron

Publikováno v: STAR Protocols, Vol 4, Iss 2, Pp 102151- (2023)

Summary: Classically, culturing mouse blastocysts with FGF4/TGF-β1, two epiblast-secreted inducers, allows for deriving trophoblast stem cells that comprise fluctuating subpopulations reflecting both pre- and post-implantation stages. However, a mor

Externí odkaz: https://doaj.org/article/b5068ece3c0e45aaabc07ad62f00f10c

A probabilistic framework for cellular lineage reconstruction using integrated single-cell 5-hydroxymethylcytosine and genomic DNA sequencing

Autor: Chatarin Wangsanuwat, Alex Chialastri, Javier F. Aldeguer, Nicolas C. Rivron, Siddharth S. Dey

Publikováno v: Cell Reports: Methods, Vol 1, Iss 4, Pp 100060- (2021)

Summary: Lineage reconstruction is central to understanding tissue development and maintenance. To overcome the limitations of current techniques that typically reconstruct clonal trees using genetically encoded reporters, we report scPECLR, a probab

Externí odkaz: https://doaj.org/article/dd37b4cc0c9d43d8b966dc193ddb084d

A pendulum of induction between the epiblast and extra-embryonic endoderm supports post-implantation progression

Autor: Erik J. Vrij, Yvonne S. Scholte op Reimer, Laury Roa Fuentes, Isabel Misteli Guerreiro, Viktoria Holzmann, Javier Frias Aldeguer, Giovanni Sestini, Bon-Kyoung Koo, Jop Kind, Clemens A. van Blitterswijk, Nicolas C. Rivron

Publikováno v: Development (Cambridge), 149(20). Company of Biologists Ltd
Development, 149(20):dev192310. Company of Biologists Ltd
Development

Embryogenesis is supported by dynamic loops of cellular interactions. Here, we create a partial mouse embryo model to elucidate the principles of epiblast (Epi) and extra-embryonic endoderm co-development (XEn). We trigger naive mouse embryonic stem

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::11b9bbcf3f5973f773d129fc7a0148b3
https://pure.knaw.nl/portal/en/publications/68d1c94d-eaa5-4672-8b61-3acbd4bcba2d

In vitro capture and characterization of embryonic rosette-stage pluripotency between naive and primed states

Autor: Emiel van Genderen, Yang Ge, Alex Maas, Hendrik Marks, Joop H. Jansen, Wilfred F. J. van IJcken, Rutger W W Brouwer, Alexander T. den Dekker, Irene Escudero, Dorota Kurek, Jente Stel, René A.M. Dirks, Lucas Verwegen, Miha Modic, Johannes Lehmann, Nicolas C. Rivron, Cindy Eleveld, Micha Drukker, Alex Neagu, Esther B. Baart, Derk ten Berge, Guido van Mierlo

Publikováno v: Nature Cell Biology, 22, 5, pp. 534-545
Nature Cell Biology, 22, 534-545
Nature Cell Biology, 22(5), 534-545. Nature Publishing Group
Nature Cell Biology, 22, 534-545. Nature Research

Following implantation, the naive pluripotent epiblast of the mouse blastocyst generates a rosette, undergoes lumenogenesis and forms the primed pluripotent egg cylinder, which is able to generate the embryonic tissues. How pluripotency progression a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::83230315c9a73bd2f7e49524d9668131
https://hdl.handle.net/2066/227169

Going high and low: on pluralism and neutrality in human embryology policy-making

Autor: Hafez Ismaili M'hamdi, Nicolas C Rivron, Eva CA Asscher

Publikováno v: Journal of Medical Ethics. BMJ Publishing Group

Formulating sound and acceptable embryo research policy remains challenging especially in a pluralistic world. This challenge has acquired a new dimension of complexity with the advent of so-called embryo models, which are derived from stem cells. In

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::99a55bd9a9f7e385d68333286170dbe3
https://pure.eur.nl/en/publications/2cdf941c-670b-4ef3-86cc-84162dd0f8ae