Defining cellular complexity in human autosomal dominant polycystic kidney disease by multimodal single cell analysis

Autor: Yoshiharu Muto, Eryn E. Dixon, Yasuhiro Yoshimura, Haojia Wu, Kohei Omachi, Nicolas Ledru, Parker C. Wilson, Andrew J. King, N. Eric Olson, Marvin G. Gunawan, Jay J. Kuo, Jennifer H. Cox, Jeffrey H. Miner, Stephen L. Seliger, Owen M. Woodward, Paul A. Welling, Terry J. Watnick, Benjamin D. Humphreys

Publikováno v: Nature Communications, Vol 13, Iss 1, Pp 1-19 (2022)

Autosomal dominant polycystic kidney disease (ADPKD) is a complicated disease that involves numerous cell types. Here the authors used a multiomics approach consisting of single nucleus transcriptomes and epigenomes to redefine cell states in ADPKD a

Externí odkaz: https://doaj.org/article/7703e88d9d3a4383b2b0dd9ea8ab76ec

Predicting regulators of epithelial cell state through regularized regression analysis of single cell multiomic sequencing

Autor: Nicolas Ledru, Parker C. Wilson, Yoshiharu Muto, Yasuhiro Yoshimura, Haojia Wu, Amish Asthana, Stefan G. Tullius, Sushrut S. Waikar, Giuseppe Orlando, Benjamin D. Humphreys

Chronic disease processes are marked by cell-specific transcriptomic and epigenomic changes. Single nucleus joint RNA- and ATAC-seq offers an opportunity to study the gene regulatory networks underpinning these changes in order to identify key regula

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a00b1c4fdbd3102fb41e24f3582494da

Alcohol Causes Lasting Differential Transcription in Drosophila Mushroom Body Neurons

Autor: Nicolas Ledru, Karla R. Kaun, Tariq Brown, Amanda G Waterman, Emily Petruccelli

Publikováno v: Genetics. 215:103-116

Cues associated with intoxication can elicit cravings for alcohol, leading to increased consumption and relapse in people recovering from alcohol use disorder. Petruccelli et al. employed genetic tools in... Repeated alcohol experiences can produce l

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::e6acc9482072b4f5c0d16c8fb41c108b
https://doi.org/10.1534/genetics.120.303101

Single cell transcriptional and chromatin accessibility profiling redefine cellular heterogeneity in the adult human kidney

Autor: Nicolas Ledru, Henrik Dimke, Yoshiharu Muto, Sushrut S. Waikar, Parker C. Wilson, Benjamin D. Humphreys, Haojia Wu

Publikováno v: Nature Communications
Nature Communications, Vol 12, Iss 1, Pp 1-17 (2021)
Muto, Y, Wilson, P C, Ledru, N, Wu, H, Dimke, H, Waikar, S S & Humphreys, B D 2021, ' Single cell transcriptional and chromatin accessibility profiling redefine cellular heterogeneity in the adult human kidney ', Nature Communications, vol. 12, 2190 . https://doi.org/10.1038/s41467-021-22368-w

The integration of single cell transcriptome and chromatin accessibility datasets enables a deeper understanding of cell heterogeneity. We performed single nucleus ATAC (snATAC-seq) and RNA (snRNA-seq) sequencing to generate paired, cell-type-specifi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5ac9ea46e36706d49ac25334453bcba5
http://europepmc.org/articles/PMC8044133

Alcohol causes lasting differential transcription inDrosophilamushroom body neurons

Autor: Emily Petruccelli, Nicolas Ledru, Karla R. Kaun

Repeated alcohol experiences can produce long-lasting memories for sensory cues associated with intoxication. These memories can ultimately trigger relapse in individuals recovering from alcohol use disorder (AUD). The molecular mechanisms by which a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9b076e70ba75199d5685102d5754fadd
https://doi.org/10.1101/752477