Zobrazeno 1 - 10
of 25
pro vyhledávání: '"Nicola J. Harris"'
Publikováno v:
Waste Management Bulletin, Vol 1, Iss 3, Pp 45-57 (2023)
Microorganisms in hypersaline potash mining byproducts and their potential environmental applications have not been extensively reported. This study reports the diverse waste-impacted microbial communities (archaea and bacteria) adapted to extreme sa
Externí odkaz:
https://doaj.org/article/e087f79c4a434828bd72a492a8051c94
Autor:
Nicola J. Harris, Grant A. Pellowe, Laura R. Blackholly, Samuel Gulaidi-Breen, Heather E. Findlay, Paula J. Booth
Publikováno v:
Open Biology, Vol 12, Iss 7 (2022)
How alpha-helical membrane proteins fold correctly in the highly hydrophobic membrane interior is not well understood. Their folding is known to be highly influenced by the lipids within the surrounding bilayer, but the majority of folding studies ha
Externí odkaz:
https://doaj.org/article/7bd8e352c1df4d9da1a25ff881457634
Autor:
Ruyu Jia, Chloe Martens, Mrinal Shekhar, Shashank Pant, Grant A. Pellowe, Andy M. Lau, Heather E. Findlay, Nicola J. Harris, Emad Tajkhorshid, Paula J. Booth, Argyris Politis
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
XylE is a bacterial xylose transporter and homologue of human glucose transporters GLUTs 1-4. HDX-MS, mutagenesis and MD simulations suggest that protonation of a conserved aspartate triggers conformational transition from outward- to inward facing s
Externí odkaz:
https://doaj.org/article/d6cb78ca4eb8432388486c3f0d334672
Publikováno v:
Frontiers in Molecular Biosciences, Vol 9 (2022)
The majority of alpha helical membrane proteins fold co-translationally during their synthesis on the ribosome. In contrast, most mechanistic folding studies address refolding of full-length proteins from artificially induced denatured states that ar
Externí odkaz:
https://doaj.org/article/253f25067bf542aab116db7a0ee6a786
Autor:
Nicola J. Harris, Eamonn Reading, Kenichi Ataka, Lucjan Grzegorzewski, Kalypso Charalambous, Xia Liu, Ramona Schlesinger, Joachim Heberle, Paula J. Booth
Publikováno v:
Scientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
Abstract Correctly folded membrane proteins underlie a plethora of cellular processes, but little is known about how they fold. Knowledge of folding mechanisms centres on reversible folding of chemically denatured membrane proteins. However, this can
Externí odkaz:
https://doaj.org/article/8f8480ca6af2428ea155c29546970aae
Autor:
null Nicola J. Harris, null Grant A. Pellowe, null Laura R. Blackholly, null Samuel Gulaidi-Breen, null Heather E. Findlay, null Paula J. Booth
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4ad1bc3682d4e0644c27067bb6876f79
https://doi.org/10.1098/rsob.220054/v2/response1
https://doi.org/10.1098/rsob.220054/v2/response1
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2433
In order to comprehend the molecular basis of transmembrane protein biogenesis, methods are required that are capable of investigating the co-translational folding of these hydrophobic proteins. Equally, in artificial cell studies, controllable metho
Publikováno v:
Cell-Free Gene Expression ISBN: 9781071619971
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::492b3e04553349652add10a030f23654
https://doi.org/10.1007/978-1-0716-1998-8_17
https://doi.org/10.1007/978-1-0716-1998-8_17
Publikováno v:
Brady, R, Harris, N J, Pellowe, G A, Breen, S G & Booth, P J 2022, ' How lipids affect the energetics of co-translational alpha helical membrane protein folding ', Biochemical Society Transactions, vol. 50, no. 1, pp. 555-567 . https://doi.org/10.1042/BST20201063
Membrane proteins need to fold with precision in order to function correctly, with misfolding potentially leading to disease. The proteins reside within a hydrophobic lipid membrane and must insert into the membrane and fold correctly, generally whil
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2315
Droplet interface bilayers (DIBs) are an emerging tool within synthetic biology that aims to recreate biological processes in artificial cells. A critical component for the utility of these bilayers is controlled flow between compartments and, notabl