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Purification, characterization and preliminary X-ray study of fumarase from Saccharomyces cerevisiae
Autor:
Gladilin Kl, Nickolai Y. Chirgadze, Vyacheslav N. Zaitsev, Irina D. Keruchenko, Jan S. Keruchenko
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. 1122:85-92
Fumarase (fumarate hydratase, EC 4.2.1.2) from Saccharomyces cerevisiae has been purified to homogeneity by a method including acetone fractionation, DEAE ion-exchange and dye-sorbent affinity chromatography. The suggested method allows fumarase puri