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Autor:
David B. Ball, Daniel J. Edwards, Nick M. Hernandez, Matthew C. Elston, Shin'ichiro Kajiyama, William H. Gerwick, Kazuhiro Irie, Minh U. Huynh
Publikováno v:
Journal of Natural Products. 73:71-74
The P450 cytochrome monooxygenase gene, ltxB, was cloned and overexpressed in Escherichia coli as a 6xHis-tagged protein. The resulting recombinant LtxB was purified by Ni-NTA affinity chromatography and characterized biochemically. Purified LtxB dem