Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Nick A Rejali"'
Publikováno v:
eLife, Vol 10 (2021)
Some RIG-I-like receptors (RLRs) discriminate viral and cellular dsRNA by their termini, and Drosophila melanogaster Dicer-2 (dmDcr-2) differentially processes dsRNA with blunt or 2 nucleotide 3’-overhanging termini. We investigated the transient k
Externí odkaz:
https://doaj.org/article/3c5c4f78c71b441f8a641c0c3cbfd1db
Publikováno v:
Nucleic Acids Research
We used stopped-flow to monitor hypochromicity for 43 oligonucleotide duplexes to study nucleic acid kinetics and extract transition-state parameters for association and dissociation. Reactions were performed in 1.0 M NaCl (for literature comparisons
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4ee955802a505fb1d8e4448405b2f191
https://doi.org/10.1093/nar/gkab205
https://doi.org/10.1093/nar/gkab205
Autor:
McKenzie Jonely, Rodrigo Noriega, Nick A Rejali, Evan Leslie, Raushan K. Singh, Brenda L. Bass
Publikováno v:
eLife, Vol 10 (2021)
Some RIG-I-like receptors (RLRs) discriminate viral and cellular dsRNA by their termini, and Drosophila melanogaster Dicer-2 (dmDcr-2) differentially processes dsRNA with blunt or 2 nucleotide 3’-overhanging termini. We investigated the transient k
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::027d823c344cc67dafbd961a7be41c49
https://elifesciences.org/articles/65810
https://elifesciences.org/articles/65810
Autor:
Raushan K, Singh, McKenzie, Jonely, Evan, Leslie, Nick A, Rejali, Rodrigo, Noriega, Brenda L, Bass
Publikováno v:
eLife
Some RIG-I-like receptors (RLRs) discriminate viral and cellular dsRNA by their termini, and Drosophila melanogaster Dicer-2 (dmDcr-2) differentially processes dsRNA with blunt or 2 nucleotide 3’-overhanging termini. We investigated the transient k
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::799aa3c725157bb83ce268b2b63aa994
https://pubmed.ncbi.nlm.nih.gov/33787495
https://pubmed.ncbi.nlm.nih.gov/33787495
Autor:
Raushan K. Singh, McKenzie Jonely, Nick A Rejali, Evan Leslie, Rodrigo Noriega, Brenda L. Bass
SUMMARYDrosophila melanogasterDicer-2 (dmDcr-2) differentially processes dsRNA with blunt or 2 nucleotide 3’-overhanging termini. We investigated the transient kinetic mechanism of these reactions using a rapid reaction stopped-flow technique and t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ea8f49684e70d74510516edbfb9c568d
https://doi.org/10.1101/2020.09.29.319475
https://doi.org/10.1101/2020.09.29.319475
Publikováno v:
Analytical biochemistry. 601
Understanding reverse transcriptase (RT) activity is critical for designing fast one-step RT-PCRs. We report a stopped-flow assay that monitors SYBR Green I fluorescence to investigate RT activity in PCR conditions. We studied the influence of PCR co
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::404c10f057465d9117b6e8996c3ae27a
https://pubmed.ncbi.nlm.nih.gov/32416095
https://pubmed.ncbi.nlm.nih.gov/32416095
Publikováno v:
Clinical chemistry. 64(5)
BACKGROUNDAllele-specific PCR is an important diagnostic tool that identifies single-nucleotide variants by preferential amplification of a particular allele, using primers that are mismatched to all but one allele variant.METHODSWe applied a fluores
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4b8fb6fdeeef0f086d0e76249f491b09
https://pubmed.ncbi.nlm.nih.gov/29444902
https://pubmed.ncbi.nlm.nih.gov/29444902
The influence of nucleotide sequence and temperature on the activity of thermostable DNA polymerases
Publikováno v:
The Journal of molecular diagnostics : JMD. 16(3)
Extension rates of a thermostable, deletion-mutant polymerase were measured from 50°C to 90°C using a fluorescence activity assay adapted for real-time PCR instruments. Substrates with a common hairpin (6-base loop and a 14-bp stem) were synthesize
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8424e16c42b2c1e40433fe844f0e55ff
https://pubmed.ncbi.nlm.nih.gov/24607271
https://pubmed.ncbi.nlm.nih.gov/24607271
Publikováno v:
Analytical biochemistry. 441(2)
DNA polymerase activity was measured by a stopped-flow assay that monitors polymerase extension using an intercalating dye. Double-stranded DNA formation during extension of a hairpin substrate was monitored at 75 °C for 2 min. Rates were determined
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::14e16dc2dbfa7ad2afa6ec821da74117
https://pubmed.ncbi.nlm.nih.gov/23872003
https://pubmed.ncbi.nlm.nih.gov/23872003