Zobrazeno 1 - 6
of 6
pro vyhledávání: '"Nicholas Vecchiarello"'
Publikováno v:
Biotechnology and Bioengineering. 119:299-314
This paper presents a straightforward approach for measuring and quantifying orthogonality directly in complex cell culture fluids (CCFs) without the requirement for tracking the retention behaviors of large sets of proteins. Null-producing CCFs were
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f2f0ccfa3da0d2cf627451a48fc50369
https://doi.org/10.1002/bit.27977
https://doi.org/10.1002/bit.27977
Autor:
John P. Trasatti, Nicholas Vecchiarello, Chaz Goodwine, Pankaj Karande, Steven M. Timmick, Akshat Mullerpatan, Divya G. Shastry, Steven M. Cramer, Divya Chandra
Publikováno v:
Journal of Chemical Technology & Biotechnology. 94:2345-2354
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::53146d2ec3723f147eb5dba1240006cb
https://doi.org/10.1002/jctb.6029
https://doi.org/10.1002/jctb.6029
Publikováno v:
Journal of chromatography. A. 1628
Recent studies have shown that by combining orthogonal, non-affinity chromatography steps, it is possible to rapidly develop efficient purification processes for molecules of interest. Here, we build upon previous work to develop a flexible framework
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7c4c98cbac69461b6e7b0439a1a5edfb
https://pubmed.ncbi.nlm.nih.gov/32822971
https://pubmed.ncbi.nlm.nih.gov/32822971
Autor:
Chaz Goodwine, Wu Sl, Catherine Bartlett Matthews, J. C. Love, Cvetkovic A, Nicholas J. Mozdzierz, Steven M. Timmick, Craig A. Mascarenhas, Kartik A. Shah, Kerry R. Love, Amos E. Lu, Alexandra D. Bonnyman, Nicholas Vecchiarello, William S. Hancock, Doherty W, Wang Ya, Richard D. Braatz, Clark Jj, Di Wu, Laura E. Crowell, Liu Y, Neil C. Dalvie, Steven M. Cramer, Liu D, Bradbury L, Brady, Noelle Colant, Alan Stockdale
Publikováno v:
Nature biotechnology
PMC
PMC
A benchtop system for protein synthesis achieves purity and potency comparable to those of marketed drugs. Conventional manufacturing of protein biopharmaceuticals in centralized, large-scale, single-product facilities is not well-suited to the agile
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ab40a9d79584eed8ae3e3868ba1e26f0
https://doi.org/10.1038/nbt.4262
https://doi.org/10.1038/nbt.4262
Autor:
Steven M. Cramer, Chaz Goodwine, Steven M. Timmick, J. Christopher Love, Laura E. Crowell, Nicholas Vecchiarello, Kerry R. Love
Publikováno v:
Biotechnology and Bioengineering. 115:2048-2060
In this study, we describe a new approach for the characterization of process-related impurities along with an in silico tool to generate orthogonal, integrated downstream purification processes for biological products. A one-time characterization of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::83c7706b35d36a05a4ffb4dd840a06c1
https://doi.org/10.1002/bit.26718
https://doi.org/10.1002/bit.26718
Autor:
Kerry R. Love, J. Christopher Love, Chaz Goodwine, Laura E. Crowell, Steven M. Cramer, Steven M. Timmick, Nicholas Vecchiarello
Publikováno v:
Biotechnology and bioengineering. 116(9)
Integrated designs of chromatographic processes for purification of biopharmaceuticals provides potential gains in operational efficiency and reductions of costs and material requirements. We describe a combined method using screening and in silico a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e84f27484605dc66198564c64b1c31c
https://pubmed.ncbi.nlm.nih.gov/31081177
https://pubmed.ncbi.nlm.nih.gov/31081177