Zobrazeno 1 - 10
of 33
pro vyhledávání: '"Nicholas E Timmins"'
Autor:
Stefanie Dietmair, Mark P Hodson, Lake-Ee Quek, Nicholas E Timmins, Peter Gray, Lars K Nielsen
Publikováno v:
PLoS ONE, Vol 7, Iss 8, p e43394 (2012)
Hek293 cells are the predominant hosts for transient expression of recombinant proteins and are used for stable expression of proteins where post-translational modifications performed by CHO cells are inadequate. Nevertheless, there is little informa
Externí odkaz:
https://doaj.org/article/808fa1a3dcfc44d5a643488fa3d2400b
Publikováno v:
Nature Biotechnology. 34:393-400
Transplantation of live cells as therapeutic agents is poised to offer new treatment options for a wide range of acute and chronic diseases. However, the biological complexity of cells has hampered the translation of laboratory-scale experiments into
Autor:
Nicholas E. Timmins, Anthony H. Davies, Peter W. Zandstra, Yonatan Y. Lipsitz, Patrick Bedford
Publikováno v:
Cell stem cell. 20(1)
New methods to manipulate gene and cell state can be used to engineer cell functionality, simplify quality assessment, and enhance manufacturability. These strategies could help overcome unresolved cell therapy manufacturing challenges and complement
Autor:
Marion E. G. Brunck, Geoffrey W. Osborne, Lars K. Nielsen, Nicholas E. Timmins, Stacey B. Andersen
Publikováno v:
Cytometry Part A. 85:1057-1064
Absolute neutrophil count (ANC) is used clinically to monitor physiological dysfunctions such as myelosuppression or infection. In the research laboratory, ANC is a valuable measure to monitor the evolution of a wide range of disease states in diseas
Autor:
Nicholas E. Timmins, Gregory S. Watson, Jolanta A. Watson, Marko Guenther, Katharine R. Trenholme, Lars Nielson, Christopher L. Peatey, Christopher L. Brown, Donald L. Gardiner
Publikováno v:
The Journal of Infectious Diseases. 208:1170-1174
Gametocytogenesis by Plasmodium falciparum is essential for transmission of the parasite from human to mosquito, yet developing gametocytes lack expression of surface proteins required for cytoadherence. Therefore, elimination from the circulation sh
Autor:
Nicholas E. Timmins, Lars K. Nielsen, Ian Alexander Aird, Michael R. Doran, Flavia Marturana, Kerry Atkinson
Publikováno v:
Cell Transplantation, Vol 21 (2012)
Cell Transplantation
Cell Transplantation
The feasibility of ex vivo blood production is limited by both biological and engineering challenges. From an engineering perspective, these challenges include the significant volumes required to generate even a single unit of a blood product, as wel
Autor:
Mark P. Hodson, Nicholas E. Timmins, Lake-Ee Quek, Stefanie Dietmair, Shana S. Jacob, Lars K. Nielsen, Panagiotis Chrysanthopoulos, Peter P. Gray
Publikováno v:
ResearcherID
Mammalian cell cultures are the predominant system for the production of recombinant proteins requiring post-translational modifications. As protein yields are a function of growth performance (among others), and performance varies greatly between cu
Autor:
M. Gunther, Michael R. Doran, Kerry Atkinson, Gary Brooke, Nicholas E. Timmins, Celena Heazlewood, Markus Kiel
Publikováno v:
Biotechnology and Bioengineering
Mesenchymal stem cells (MSC) are emerging as a leading cellular therapy for a number of diseases. However, for such treatments to become available as a routine therapeutic option, efficient and cost-effective means for industrial manufacture of MSC a
Publikováno v:
Tissue Engineering Part C: Methods. 17:1131-1137
Provision of a safe and secure supply of transfusible red blood cells (RBC) is a major global health challenge, and it has been proposed that manufactured RBC could help to alleviate the constraints of the current donor system. Several substantial ch
Publikováno v:
Cytotherapy. 13:366-377
Background aims Despite the availability of modern antibiotics/antimycotics and cytokine support, neutropenic infection accounts for the majority of chemotherapy-associated deaths. While transfusion support with donor neutrophils is possible, cost an