Can-Seq: a PCR and DNA sequencing strategy for identifying new alleles of known and candidate genes

Autor: Jiangling Cao, Nial R. Gursanscky, Stephen J. Fletcher, Anne Sawyer, Mehershad Wadia, Lachlan McKeough, Marek Coleman, Uwe Dressel, Christelle Taochy, Neena Mitter, Hervé Vaucheret, Bernard J. Carroll

Publikováno v: Plant Methods, Vol 16, Iss 1, Pp 1-12 (2020)

Abstract Background Forward genetic screens are a powerful approach for identifying the genes contributing to a trait of interest. However, mutants arising in genes already known can obscure the identification of new genes contributing to the trait.

Externí odkaz: https://doaj.org/article/89d6dbecf9734a4198a7e8fefd8e0267

DEFECTIVE EMBRYO AND MERISTEMS genes are required for cell division and gamete viability in Arabidopsis

Autor: Bernard J. Carroll, Filipe Borges, John L. Bowman, Christopher W. G. Mann, Christopher A. Brosnan, Venkatesan Sundaresan, Thierry G. A. Lonhienne, Chin Hong Lee, Bostjan Kobe, Nial R. Gursanscky, Quy A. Ngo, Nathaniel P. Hawker, Raphael Mercier, Laurence Cromer, Christelle Taochy, Louisa Matthew, Peer M. Schenk, Jonathan R. Peters

Publikováno v: PLoS Genetics
PLoS Genetics, Vol 17, Iss 5, p e1009561 (2021)
PLoS genetics, vol 17, iss 5
PLoS Genetics, Public Library of Science, 2021, 17 (5), pp.e1009561. ⟨10.1371/journal.pgen.1009561⟩

The DEFECTIVE EMBRYO AND MERISTEMS 1 (DEM1) gene encodes a protein of unknown biochemical function required for meristem formation and seedling development in tomato, but it was unclear whether DEM1’s primary role was in cell division or alternativ

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c2f84d7eb99b05c6ba937422dc6c1169
https://hdl.handle.net/21.11116/0000-000A-9B54-921.11116/0000-000A-9B52-B

MOL 1 is required for cambium homeostasis in Arabidopsis

Autor: Nial R. Gursanscky, Martina Laaber‐Schwarz, Virginie Jouannet, Karin Grünwald, Pablo Sanchez, Thomas Greb

Publikováno v: The Plant Journal

Summary Plants maintain pools of pluripotent stem cells which allow them to constantly produce new tissues and organs. Stem cell homeostasis in shoot and root tips depends on negative regulation by ligand–receptor pairs of the CLE peptide and leuci

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8a86ed34cbf0450c318c1becd94cc356
https://doi.org/10.1111/tpj.13169

A Genetic Screen for Impaired Systemic RNAi Highlights the Crucial Role of DICER-LIKE 2

Autor: Bernard J. Carroll, John L. Bowman, Matthew R. Tucker, Nial R. Gursanscky, Jiangling Cao, Christelle Taochy, Neena Mitter, Stephen J. Fletcher, Anna M. G. Koltunow, Hervé Vaucheret, Uwe Dressel

Publikováno v: Plant Physiology
Plant Physiology, American Society of Plant Biologists, 2017, 175 (3), pp.1424-1437. ⟨10.1104/pp.17.01181⟩

International audience; Posttranscriptional gene silencing (PTGS) of transgenes involves abundant 21-nucleotide small interfering RNAs (siRNAs) and low-abundance 22-nucleotide siRNAs produced fromdouble-stranded RNA (dsRNA) by DCL4 and DCL2, respecti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::25fc9b542c22906e213083bcd785b3cf
https://pubmed.ncbi.nlm.nih.gov/28928141

Mechanism of Small RNA Movement

Autor: Bernard J. Carroll, Nial R. Gursanscky

Publikováno v: Short and Long Distance Signaling ISBN: 9781441915313

Higher plants rely on the movement of signals between cells to coordinate gene expression, to define cell type and polarity during development, to respond to environmental stimuli, and for defence against pathogen infection. These signals can take a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::d0de450babc3bdaa8f6b596ff78bd819
https://doi.org/10.1007/978-1-4419-1532-0_5