Enhancing powdery mildew resistance in soybean by targeted mutation of MLO genes using the CRISPR/Cas9 system

Autor: Thao Phuong Bui, Huy Le, Dong Thi Ta, Cuong Xuan Nguyen, Ngoc Thu Le, Truong Thi Tran, Phuong Van Nguyen, Gary Stacey, Minviluz G. Stacey, Ngoc Bich Pham, Ha Hoang Chu, Phat Tien Do

Publikováno v: BMC Plant Biology, Vol 23, Iss 1, Pp 1-13 (2023)

Abstract Background Powdery mildew is a major disease that causes great losses in soybean yield and seed quality. Disease-resistant varieties, which are generated by reducing the impact of susceptibility genes through mutation in host plants, would b

Externí odkaz: https://doaj.org/article/627f35dd376b4b78a66039a3ec773b28

An Efficient Hairy Root System for Validation of Plant Transformation Vector and CRISPR/Cas Construct Activities in Cucumber (Cucumis sativus L.)

Autor: Doai Van Nguyen, Trang Thi-Huyen Hoang, Ngoc Thu Le, Huyen Thi Tran, Cuong Xuan Nguyen, Yong-Hwan Moon, Ha Hoang Chu, Phat Tien Do

Publikováno v: Frontiers in Plant Science, Vol 12 (2022)

Hairy root induction system has been applied in various plant species as an effective method to study gene expression and function due to its fast-growing and high genetic stability. Recently, these systems have shown to be an effective tool to evalu

Externí odkaz: https://doaj.org/article/990dd4b6eb1a4cc5bff17fca6c0adae7

Nanodiamond enhances immune responses in mice against recombinant HA/H7N9 protein

Autor: Ngoc Bich Pham, Thuong Thi Ho, Giang Thu Nguyen, Thuy Thi Le, Ngoc Thu Le, Huan-Cheng Chang, Minh Dinh Pham, Udo Conrad, Ha Hoang Chu

Publikováno v: Journal of Nanobiotechnology, Vol 15, Iss 1, Pp 1-12 (2017)

Abstract Background The continuing spread of the newly emerged H7N9 virus among poultry in China, as well as the possibility of human-to-human transmission, has attracted numerous efforts to develop an effective vaccine against H7N9. The use of nanop

Externí odkaz: https://doaj.org/article/b1b6861596544453ba1a3624751dbc6d

Developing a robust in vivo hairy root system for assessing transgene expression and genome editing efficiency in papaya

Autor: Trang Huyen Thi Hoang, Nhung Hong Nguyen, Linh Thi Nguyen, Thao Phuong Bui, Ngoc Thu Le, Nhan Trong Dao, Muriel Monteiro, Ngoc Bich Pham, Attila Molnar, Ha Hoang Chu, Phat Tien Do

Publikováno v: Thi Huyen Hoang, T, Nguyen, N H, Thi Nguyen, L, Bui, T P, Le, N T, Trong Dao, N, Monteiro, M, Pham, N B, Molnar, A, Chu, H H & Do, P T 2022, ' Developing a robust in vivo hairy root system for assessing transgene expression and genome editing efficiency in papaya ', Plant Cell, Tissue and Organ Culture . https://doi.org/10.1007/s11240-022-02421-2

Papaya is one of the most important fruits in tropical and subtropical countries. However, genetic improvement has had limited success to date due to time-consuming and complex transformation and regeneration technologies, as well as a lack of reprod

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0790f1a8b8c4816ebcab72e1d8b959d1
https://www.pure.ed.ac.uk/ws/files/309192336/The_clean_manuscript.pdf

A sequential transformation method for validating soybean genome editing by CRISPR/Cas9 system

Autor: Duy Dinh, Trinh, Ngoc Thu, Le, Thao Phuong, Bui, Thao Nhu Thi, Le, Cuong Xuan, Nguyen, Ha Hoang, Chu, Phat Tien, Do

Publikováno v: Saudi Journal of Biological Sciences. 29:103420

This study was performed to evaluate the sequential transformation for soybean genome editing using the CRISPR/Cas9 system as well as to show a strategy for examining the activity of CRISPR/Cas9 constructs, especially the designed guide RNAs (gRNAs).

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5774d52d13ff19718a65c5af30f81be4
https://doi.org/10.1016/j.sjbs.2022.103420