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of 1 332
pro vyhledávání: '"New England Biolabs"'
Autor:
New England Biolabs
The NEBNext Ultra II Directional RNA Library Prep Kit for Illumina contains the enzymes and buffers required to convert a broad range of input amounts of RNA into high quality directional (strand-specific) libraries for next-generation sequencing on
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::42a6036af24d28324096b36f48dab0ba
https://doi.org/10.17504/protocols.io.6qpvredyblmk/v3
https://doi.org/10.17504/protocols.io.6qpvredyblmk/v3
Autor:
New England Biolabs
Protocol for high efficiency heat shock transformation of competent E. coli cells. Source: New England Biolabs
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::56eaf05b46d55bab3997a13d719bceab
https://doi.org/10.17504/protocols.io.14egn2bdzg5d/v1
https://doi.org/10.17504/protocols.io.14egn2bdzg5d/v1
Autor:
New England Biolabs, Ken Christensen
The following is a "typical" restriction endonuclease reaction. Please see the "guidelines" tab below for the NEB tips on optimizing restriction digests.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5fcb917b8b5654be1601f5fe5b44e77c
https://doi.org/10.17504/protocols.io.b4hvqt66
https://doi.org/10.17504/protocols.io.b4hvqt66
This protocol details NEBNext® Immune Sequencing Kit (Human). https://www.neb.com/-/media/nebus/files/manuals/manuale6320.pdf?rev=1dca310ed12a4e89b322063d31db030d&hash=D4D7E9FBED23CC7448447137DBFF8C0B
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::15bf5fe0d3a08659b73114a3a26fd1de
https://doi.org/10.17504/protocols.io.b3ftqjnn
https://doi.org/10.17504/protocols.io.b3ftqjnn
Autor:
New England Biolabs
This protocol details methods for the NEBNext® ARTIC SARS-CoV-2 FS Library Prep Kit (Illumina®), NEB #E7658S/L 24/96 reactions. This protocol follows an alternate variant-tolerant approach for targeting SARS-CoV-2 by utilizing NEBNext VarSkip Short
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::31ebc7e7b24b3aeb54a0dad18279fedb
https://doi.org/10.17504/protocols.io.bx9apr2e
https://doi.org/10.17504/protocols.io.bx9apr2e
Autor:
New England Biolabs
This protocol details methods for the NEBNext® ARTIC SARS-CoV-2 Companion Kit (Oxford Nanopore Technologies®), NEB #E7660S/L 24/96 reactions and the NEBNext VarSkip Short SARS-CoV-2 Primer Mix 1 and 2 released in Sep 2021. This protocol does not in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c799bf48de14c252c07113f27a0ab751
https://doi.org/10.17504/protocols.io.bxtupnnw
https://doi.org/10.17504/protocols.io.bxtupnnw
Autor:
New England Biolabs
This protocol details methods for the NEBNext® ARTIC SARS-CoV-2 FS Library Prep Kit (Illumina®), NEB #E7658S/L 24/96 reactions. This protocol does not include a cleanup step for each sample after cDNA synthesis and ligation. Performing the cleanup
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c6b3caf2ceb4a29136085d04db35f606
https://doi.org/10.17504/protocols.io.bvufn6tn
https://doi.org/10.17504/protocols.io.bvufn6tn
Autor:
New England Biolabs
This protocol details methods for the NEBNext® ARTIC SARS-CoV-2 Companion Kit (Oxford Nanopore Technologies®), NEB #E7660S/L 24/96 reactions. This protocol does not include a cleanup and normalization step for each sample after cDNA synthesis. Perf
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::26d45ea6cf02fd92c919c9d84d661b1a
https://doi.org/10.17504/protocols.io.bt6jnrcn
https://doi.org/10.17504/protocols.io.bt6jnrcn
Autor:
New England Biolabs
Protocol for DNA Cleanup and Concentration Using the Monarch® PCR & DNA Cleanup Kit (5 μg) (NEB #T1030). You can also download the Quick Protocol Card here.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c4980e8fb6c75e6b2b7af536e15a3d7e
https://doi.org/10.17504/protocols.io.btd3ni8n
https://doi.org/10.17504/protocols.io.btd3ni8n
Autor:
New England Biolabs
The Monarch PCR & DNA Cleanup Kit protocolcan be modified to enable the purification of ssDNA, oligonucleotides, and other small DNA fragments. The following modified protocol utilizes the same columns and bind/wash/elute workflow of the Monarch PCR
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::64986acbca8a239453f7b073d509d348
https://doi.org/10.17504/protocols.io.btd4ni8w
https://doi.org/10.17504/protocols.io.btd4ni8w