Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Neva Ivancic"'
Autor:
T. C. Elleman, Peter A. Hoyne, John D. Bentley, Neil M. McKern, Leah J. Cosgrove, Colin W. Ward, Neva Ivancic
Publikováno v:
FEBS Letters. 479:15-18
Insulin receptors (IRs) that are truncated at the end of the ectodomain form dimers that bind insulin with different characteristics to wild type receptors. These soluble IRs have lowered affinity for insulin compared with full-length IR, and exhibit
Autor:
Geoffrey J. Howlett, Robyn L. Malby, Michael C. Lawrence, J. Bruce Caldwell, L. Clem Gruen, Alexander A. Kortt, Robert G. Webster, Peter M. Colman, Neva Ivancic, Peter J. Hudson
Publikováno v:
European Journal of Biochemistry. 221:151-157
The single-chain antibody variable fragment (scFv), with a 15-residue polypeptide linker (Gly4Ser)3, of monoclonal antibody NC10 was expressed in Escherichia coli and purified to homogeneity. This scFv molecule, refolded from 6 M guanidine hydrochlor
Publikováno v:
Protein Expression and Purification. 3:434-440
The Escherichia coli gene which encodes N-acetylneuraminic acid aldolase was isolated by the polymerase chain reaction, cloned into the inducible expression vector pTTQ18, and overexpressed in E. coli. The high yield of aldolase was achieved through
Autor:
Barbara E. Power, Alexander A. Kortt, Neva Ivancic, Vincent R. Harley, Peter J. Hudson, Robert Alexander Irving, Robert G. Webster
Publikováno v:
Gene. 113:95-99
We have constructed a temperature-inducible Escherichia coli expression vector (pPOW) for enhanced secretion of antibody (Ab) domains and other foreign proteins. The vector contains the lambda pRpL promoters in tandem, and the cI857 gene encoding the
Autor:
Neva Ivancic, K. Siddle, Colin W. Ward, George O. Lovrecz, Neil M. McKern, John D. Bentley, L.J. Lawrence, Christine P. Robinson, P.A. Tulloch, Leah J. Cosgrove
Publikováno v:
Journal of structural biology. 125(1)
The insulin receptor (IR) is a four-chain, transmembrane dimer held together by disulfide bonds. To gain information about the molecular envelope and the organization of its domains, single-molecule images of the IR ectodomain and its complexes with
Autor:
Thomas P. J. Garrett, M. J. Frenkel, T. C. Elleman, Leah J. Cosgrove, Neva Ivancic, George O. Lovrecz, Amanda J. Verkuylen, Neil M. McKern, John D. Bentley, Meizhen Lou, Colin W. Ward
Publikováno v:
Protein science : a publication of the Protein Society. 6(12)
The insulin-like growth factor-1 receptor (IGF-1R) is a tyrosine kinase receptor of central importance in cell proliferation. A fragment (residues 1-462) comprising the L1-cysteine rich-L2 domains of the human IGF-1R ectodomain has been overexpressed
Autor:
Raymond S. Norton, Barbara E. Power, J. Bruce Caldwell, Peter J. Hudson, Neva Ivancic, Robin E. Guthrie, L. Clem Gruen, Mark G. Hinds, Alexander A. Kortt
Publikováno v:
Journal of protein chemistry. 14(3)
The VH domain of anti-influenza neuraminidase antibody NC41, with and without a C-terminal hydrophilic marker peptide (FLAG), has been expressed in high yield (15-27 mg/L) in Escherichia coli. Both forms were secreted into the periplasm where they fo
Autor:
Barbara E. Power, Alexander A. Kortt, Robyn L. Malby, Neva Ivancic, Robert G. Webster, Peter M. Colman, L. Clem Gruen, Vincent R. Harley, J. Bruce Caldwell, Peter J. Hudson, Glenn G. Lilley
Publikováno v:
Proteins. 16(1)
The variable heavy (VH) and variable light (VL) genes of NC10, a monoclonal antibody with specificity toward N9 neuraminidase (NA), were cloned and sequenced. A single chain Fv (scFv) fragment of NC10, consisting of VH and VL domains joined by a pept