Zobrazeno 1 - 10
of 28
pro vyhledávání: '"Neta Agmon"'
Autor:
Gregory W. Goldberg, Jeffrey M. Spencer, David O. Giganti, Brendan R. Camellato, Neta Agmon, David M. Ichikawa, Jef D. Boeke, Marcus B. Noyes
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-16 (2021)
The PAM specificity of SpCas9 can be altered with positive selection during directed evolution. Here the authors use simultaneous positive and negative selection to improve activity on NAG PAMs while reducing activity on NGG PAMs.
Externí odkaz:
https://doaj.org/article/3e675de09c284972a7413a9b4147d955
Autor:
Sonja Billerbeck, James Brisbois, Neta Agmon, Miguel Jimenez, Jasmine Temple, Michael Shen, Jef D. Boeke, Virginia W. Cornish
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-12 (2018)
A bottleneck to engineered multicellular systems is the lack of a scalable signalling language. Here the authors use fungal mating peptide and GPCR pairs to build functional cell-to-cell communication links.
Externí odkaz:
https://doaj.org/article/2301b0715395466f978cf9be280cb30d
Autor:
Ze-Xiong Xie, Leslie A. Mitchell, Hui-Min Liu, Bing-Zhi Li, Duo Liu, Neta Agmon, Yi Wu, Xia Li, Xiao Zhou, Bo Li, Wen-Hai Xiao, Ming-Zhu Ding, Ying Wang, Ying-Jin Yuan, Jef D. Boeke
Publikováno v:
G3: Genes, Genomes, Genetics, Vol 8, Iss 1, Pp 173-183 (2018)
Rapid and highly efficient mating-type switching of Saccharomyces cerevisiae enables a wide variety of genetic manipulations, such as the construction of strains, for instance, isogenic haploid pairs of both mating-types, diploids and polyploids. We
Externí odkaz:
https://doaj.org/article/05bbc6daf9c74c21b12390cec18c1768
Autor:
Sonja Billerbeck, James Brisbois, Neta Agmon, Miguel Jimenez, Jasmine Temple, Michael Shen, Jef D. Boeke, Virginia W. Cornish
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-1 (2019)
The original version of this Article omitted a declaration from the Competing Interests statement, which should have included the following: ‘J.D.B. is a founder and Director of the following: Neochromosome, Inc., the Center of Excellence for Engin
Externí odkaz:
https://doaj.org/article/44d7c00317b5409e8f34ccfa423fb569
Autor:
Shay Ben-Aroya, Neta Agmon, Karen Yuen, Teresa Kwok, Kirk McManus, Martin Kupiec, Philip Hieter
Publikováno v:
PLoS Genetics, Vol 6, Iss 2, p e1000852 (2010)
To expand the known spectrum of genes that maintain genome stability, we screened a recently released collection of temperature sensitive (Ts) yeast mutants for a chromosome instability (CIN) phenotype. Proteasome subunit genes represented a major fu
Externí odkaz:
https://doaj.org/article/b59b9a78e968446bb75293b8f4849137
Autor:
Jef D. Boeke, Marcus B. Noyes, Brendan Camellato, David M. Ichikawa, Gregory W. Goldberg, Neta Agmon, Jeffrey M. Spencer, David Giganti
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-16 (2021)
Nature Communications
Nature Communications
The widely used Streptococcus pyogenes Cas9 (SpCas9) nuclease derives its DNA targeting specificity from protein-DNA contacts with protospacer adjacent motif (PAM) sequences, in addition to base-pairing interactions between its guide RNA and target D
Autor:
Yu Zhao, Camila Coelho, Amanda L. Hughes, Luciana Lazar-Stefanita, Sandy Yang, Aaron N. Brooks, Roy S. K. Walker, Weimin Zhang, Stephanie Lauer, Cindy Hernandez, Leslie A. Mitchell, Neta Agmon, Yue Shen, Joseph Sall, Viola Fanfani, Anavi Jalan, Jordan Rivera, Feng-Xia Liang, Giovanni Stracquadanio, Lars M. Steinmetz, Yizhi Patrick Cai, Jef D. Boeke
The Sc2.0 project is building a eukaryotic synthetic genome from scratch, incorporating thousands of designer features. A major milestone has been achieved with the assembly of all individual Sc2.0 chromosomes. Here, we describe the consolidation of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1e3a2c373b173cbc37bf13b941f94497
https://doi.org/10.1101/2022.04.11.486913
https://doi.org/10.1101/2022.04.11.486913
Autor:
Jef D. Boeke, Paolo Mita, David J. Kahler, Chi Yun, Aleksandra Wudzinska, David Fenyö, Xiaoji Sun, Neta Agmon, Donghui Li, Sarah Keegan, Joel S. Bader
Publikováno v:
Nature structural & molecular biology
Long interspersed element-1 (LINE-1, or L1) is the only autonomous retrotransposon that is active in human cells. Different host factors have been shown to influence L1 mobility; however, systematic analyses of these factors are limited. Here, we dev
Autor:
Jef D. Boeke, Paolo Mita, Neta Agmon, Itai Yanai, Maayan Baron, Tobias Schraink, Zuojian Tang, James A. Martin, Jun Chen, Jasmine Temple, David Fenyö, Benjamin P. Tu
Publikováno v:
Nucleic Acids Research
Cross-species pathway transplantation enables insight into a biological process not possible through traditional approaches. We replaced the enzymes catalyzing the entire Saccharomyces cerevisiae adenine de novo biosynthesis pathway with the human pa
Autor:
Duo Liu, Yi Wu, Wen Hai Xiao, Xia Li, Neta Agmon, Ying Wang, Bo Li, Jef D. Boeke, Leslie A. Mitchell, Ze-Xiong Xie, Bing-Zhi Li, Ming Zhu Ding, Xiao Zhou, Ying-Jin Yuan, Hui Min Liu
Publikováno v:
G3: Genes, Genomes, Genetics, Vol 8, Iss 1, Pp 173-183 (2018)
G3: Genes|Genomes|Genetics
G3: Genes|Genomes|Genetics
Rapid and highly efficient mating-type switching of Saccharomyces cerevisiae enables a wide variety of genetic manipulations, such as the construction of strains, for instance, isogenic haploid pairs of both mating-types, diploids and polyploids. We