Zobrazeno 1 - 10
of 54
pro vyhledávání: '"Neta, Dean"'
Autor:
Shuai Chen, Cai-Xia Pei, Si Xu, Hanjie Li, Yi-Shi liu, Yicheng Wang, Cheng Jin, Neta Dean, Xiao-Dong Gao
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-10 (2024)
Abstract The eukaryotic asparagine (N)-linked glycan is pre-assembled as a fourteen-sugar oligosaccharide on a lipid carrier in the endoplasmic reticulum (ER). Seven sugars are first added to dolichol pyrophosphate (PP-Dol) on the cytoplasmic face of
Externí odkaz:
https://doaj.org/article/21c0b5b4b0ea4b79b4eb009bd7c3fd38
Autor:
Meng-Hai Xiang, Xin-Xin Xu, Chun-Di Wang, Shuai Chen, Si Xu, Xiang-Yang Xu, Neta Dean, Ning Wang, Xiao-Dong Gao
Publikováno v:
Communications Biology, Vol 5, Iss 1, Pp 1-12 (2022)
Despite the conservation of N-glycosylation, human and yeast Alg2 structures have diverged with distinct ER-binding topologies. The human enzyme is more stable than the yeast orthologue, and its activity is modulated by the concentration of donor or
Externí odkaz:
https://doaj.org/article/f0d9aaf2f58a4ed1933813f69af00bdc
Publikováno v:
Frontiers in Cell and Developmental Biology, Vol 10 (2022)
The second step of eukaryotic lipid-linked oligosaccharide (LLO) biosynthesis is catalyzed by the conserved ALG13/ALG14 heterodimeric UDP-N-acetylglucosamine transferase (GnTase). In humans, mutations in ALG13 or ALG14 lead to severe neurological dis
Externí odkaz:
https://doaj.org/article/eb92fe9b2bd9400eadf9271250d637b1
Autor:
Sheng-Tao Li, Tian-Tian Lu, Xin-Xin Xu, Yi Ding, Zijie Li, Toshihiko Kitajima, Neta Dean, Ning Wang, Xiao-Dong Gao
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-11 (2019)
Attachment of the oligosaccharide Man9GlcNAc2 is required for glycoprotein folding and secretion but synthesizing this compound for structural and functional studies has remained challenging. Here, the authors achieve efficient Man9GlcNAc2 synthesis
Externí odkaz:
https://doaj.org/article/29a5c7cd42d945a9960434571e434f91
Publikováno v:
Bio-Protocol, Vol 8, Iss 8 (2018)
Candida albicans is the most prevalent and important human fungal pathogen. The advent of CRISPR as a means of gene editing has greatly facilitated genetic analysis in C. albicans. Here, we describe a detailed step-by-step procedure to construct and
Externí odkaz:
https://doaj.org/article/53bddc4e5a1648a4b246d102cad9c5ff
Publikováno v:
mSphere, Vol 2, Iss 2 (2017)
ABSTRACT The clustered regularly interspaced short palindromic repeat system with CRISPR-associated protein 9 nuclease (CRISPR/Cas9) has emerged as a versatile tool for genome editing in Candida albicans. Mounting evidence from other model systems su
Externí odkaz:
https://doaj.org/article/8f2e2829062f40ec87ede26aa3660356
Publikováno v:
Frontiers in cell and developmental biology. 10
The second step of eukaryotic lipid-linked oligosaccharide (LLO) biosynthesis is catalyzed by the conserved ALG13/ALG14 heterodimeric UDP
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::b84b72632f3aa8941736438264a2295d
https://pubmed.ncbi.nlm.nih.gov/36200043
https://pubmed.ncbi.nlm.nih.gov/36200043
Publikováno v:
Cell Structure and Function. 45:77-92
Endocytic cargos are transported to recycling endosomes (RE) but how these sorting platforms are generated is not well understood. Here we describe our biochemical and live imaging studies of the conserved MON2-DOPEY complex in RE formation. MON2 mai
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::feb2c4027f76b2259738b06a926aca6f
https://doi.org/10.1247/csf.20012
https://doi.org/10.1247/csf.20012
Publikováno v:
Genetics
Candida albicans cell wall glycoproteins, and in particular their mannose-rich glycans, are important for maintaining cellular integrity as well as host recognition, adhesion, and immunomodulation. The asparagine (N)-linked mannose outer chain of the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8bf50da03e0ee5f56d8ff7a0ea379020
https://europepmc.org/articles/PMC9071539/
https://europepmc.org/articles/PMC9071539/
Autor:
Neta Dean, Xin-Xin Xu, Chun-Di Wang, Ning Wang, Shuai Chen, Si Xu, Xiang-Yang Xu, Xiao-Dong Gao, Meng-Hai Xiang
N-glycosylation starts with the biosynthesis of lipid-linked oligosaccharide (LLO) on the endoplasmic reticulum. Alg2 mannosyltransferase adds both the α1,3- and α1,6-Man onto ManGlcNAc2-pyrophosphate-dolichol (M1Gn2-PDol) in either order to genera
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b7dd679818c59a03d79a300ce35b0e87
https://doi.org/10.21203/rs.3.rs-871713/v1
https://doi.org/10.21203/rs.3.rs-871713/v1