Integrity assay for messenger RNA in mouse and human brain samples and synaptosomal preparations.

Autor: Bujanauskiene D; VU LSC-EMBL Partnership Institute for Genome Editing Technologies, Life Sciences Center, Vilnius University, Vilnius, Lithuania.; Institute of Biosciences, Life Sciences Center, Vilnius University, Vilnius, Lithuania., Merkevicius K; Institute of Biosciences, Life Sciences Center, Vilnius University, Vilnius, Lithuania.; Clinic of Pediatrics, Institute of Clinical Medicine, Faculty of Medicine, Vilnius University, Vilnius, Lithuania., Kuliesiute U; VU LSC-EMBL Partnership Institute for Genome Editing Technologies, Life Sciences Center, Vilnius University, Vilnius, Lithuania.; Institute of Biosciences, Life Sciences Center, Vilnius University, Vilnius, Lithuania., Denkovskij J; Department of Regenerative Medicine, Center for Innovative Medicine, Vilnius, Lithuania., Kutanovas S; VU LSC-EMBL Partnership Institute for Genome Editing Technologies, Life Sciences Center, Vilnius University, Vilnius, Lithuania., Luksys G; Center of Neurosurgery, Vilnius University Hospital Santaros Klinikos, Vilnius, Lithuania.; Clinic of Neurology and Neurosurgery, Faculty of Medicine, Vilnius University, Vilnius, Lithuania., Rocka S; Center of Neurosurgery, Vilnius University Hospital Santaros Klinikos, Vilnius, Lithuania.; Clinic of Neurology and Neurosurgery, Faculty of Medicine, Vilnius University, Vilnius, Lithuania., Bernotiene E; Department of Regenerative Medicine, Center for Innovative Medicine, Vilnius, Lithuania., Neniskyte U; VU LSC-EMBL Partnership Institute for Genome Editing Technologies, Life Sciences Center, Vilnius University, Vilnius, Lithuania.; Institute of Biosciences, Life Sciences Center, Vilnius University, Vilnius, Lithuania.

Publikováno v: IScience [iScience] 2024 Jun 29; Vol. 27 (8), pp. 110419. Date of Electronic Publication: 2024 Jun 29 (Print Publication: 2024).

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Genetically encoded phosphatidylserine biosensor for in vitro, ex vivo and in vivo labelling.

Autor: Dirvelyte E; VU LSC-EMBL Partnership Institute for Genome Editing Technologies, Life Sciences Center, Vilnius University, Vilnius, Lithuania., Bujanauskiene D; VU LSC-EMBL Partnership Institute for Genome Editing Technologies, Life Sciences Center, Vilnius University, Vilnius, Lithuania.; Institute of Bioscience, Life Sciences Center, Vilnius University, Vilnius, Lithuania., Jankaityte E; VU LSC-EMBL Partnership Institute for Genome Editing Technologies, Life Sciences Center, Vilnius University, Vilnius, Lithuania.; Institute of Biochemistry, Life Sciences Center, Vilnius University, Vilnius, Lithuania., Daugelaviciene N; VU LSC-EMBL Partnership Institute for Genome Editing Technologies, Life Sciences Center, Vilnius University, Vilnius, Lithuania., Kisieliute U; Institute of Bioscience, Life Sciences Center, Vilnius University, Vilnius, Lithuania., Nagula I; VU LSC-EMBL Partnership Institute for Genome Editing Technologies, Life Sciences Center, Vilnius University, Vilnius, Lithuania., Budvytyte R; VU LSC-EMBL Partnership Institute for Genome Editing Technologies, Life Sciences Center, Vilnius University, Vilnius, Lithuania.; Institute of Biochemistry, Life Sciences Center, Vilnius University, Vilnius, Lithuania., Neniskyte U; VU LSC-EMBL Partnership Institute for Genome Editing Technologies, Life Sciences Center, Vilnius University, Vilnius, Lithuania. urte.neniskyte@gmc.vu.lt.; Institute of Bioscience, Life Sciences Center, Vilnius University, Vilnius, Lithuania. urte.neniskyte@gmc.vu.lt.

Publikováno v: Cellular & molecular biology letters [Cell Mol Biol Lett] 2023 Jul 27; Vol. 28 (1), pp. 59. Date of Electronic Publication: 2023 Jul 27.

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Microglia states and nomenclature: A field at its crossroads.

Autor: Paolicelli RC; Department of Biomedical Sciences, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland. Electronic address: rosachiara.paolicelli@unil.ch., Sierra A; Achucarro Basque Center for Neuroscience, Glial Cell Biology Lab, Leioa, Spain; Department of Neuroscience, University of the Basque Country EHU/UPV, Leioa, Spain; Ikerbasque Foundation, Bilbao, Spain. Electronic address: amanda.sierra@ehu.eus., Stevens B; Broad Institute of MIT and Harvard, Cambridge, MA, USA; Howard Hughes Medical Institute, (HHMI), MD, USA; Boston Children's Hospital, Boston, MA, USA. Electronic address: beth.stevens@childrens.harvard.edu., Tremblay ME; Centre de recherche du CHU de Québec-Université Laval, Québec City, QC, Canada; Department of Neurology and Neurosurgery, McGill University, Montréal, QC, Canada; Division of Medical Sciences, University of Victoria, Victoria, BC, Canada; Center for Advanced Materials and Related Technology (CAMTEC), University of Victoria, Victoria, BC, Canada; Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC, Canada. Electronic address: evetremblay@uvic.ca., Aguzzi A; Institute of Neuropathology, University of Zurich, Zurich, Switzerland., Ajami B; Department of Molecular Microbiology & Immunology, Department of Behavioral and Systems Neuroscience, Oregon Health & Science University School of Medicine, Portland, OR, USA., Amit I; Department of Systems Immunology, Weizmann Institute of Science, Rehovot, Israel., Audinat E; Institut de Génomique Fonctionnelle, Université de Montpellier, CNRS, INSERM, Montpellier, France., Bechmann I; Institute of Anatomy, University of Leipzig, Leipzig, Germany., Bennett M; Children's Hospital of Philadelphia, Department of Psychiatry, Department of Pediatrics, Division of Child Neurology, Philadelphia, PA, USA., Bennett F; Children's Hospital of Philadelphia, University of Pennsylvania, Philadelphia, PA, USA., Bessis A; École Normale Supérieure, Institut National de la Santé et de la Recherche Médicale, Centre National de la Recherche Scientifique, Paris Sciences et Lettres Research University, Paris, France., Biber K; Neuroscience Discovery, AbbVie Deutschland GmbH, Ludwigshafen, Germany., Bilbo S; Departments of Psychology & Neuroscience, Neurobiology, and Cell Biology, Duke University, Durham, NC, USA., Blurton-Jones M; Center for the Neurobiology of Learning and Memory, UCI MIND, University of California, Irvine, CA, USA., Boddeke E; Department Biomedical Sciences of Cells & Systems, Section Molecular Neurobiology, University of Groningen, University Medical Center, Groningen, the Netherlands., Brites D; Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Lisbon, Portugal., Brône B; BIOMED Research Institute, University of Hasselt, Hasselt, Belgium., Brown GC; Department of Biochemistry, University of Cambridge, Cambridge, UK., Butovsky O; Ann Romney Center for Neurologic Diseases, Department Neurology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA., Carson MJ; Center for Glial-Neuronal Interactions, Division of Biomedical Sciences, University of California Riverside School of Medicine, Riverside, CA, USA., Castellano B; Unidad de Histología Medica, Depto. Biología Celular, Fisiología e Inmunología, Barcelona, Spain; Instituto de Neurociencias, Universidad Autónoma de Barcelona, Barcelona, Spain., Colonna M; Department of Pathology and Immunology, Washington University School of Medicine in St. Louis, St. Louis, MO, USA., Cowley SA; James and Lillian Martin Centre for Stem Cell Research, Sir William Dunn School of Pathology, University of Oxford, Oxford, UK., Cunningham C; School of Biochemistry & Immunology, Trinity Biomedical Sciences Institute, Trinity College, Dublin, Republic of Ireland; Trinity College Institute of Neuroscience, Trinity College, Dublin, Republic of Ireland., Davalos D; Department of Neurosciences, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, USA; Department of Molecular Medicine, Cleveland Clinic Lerner College of Medicine, Case Western Reserve University, Cleveland, OH, USA., De Jager PL; Center for Translational & Computational Neuroimmunology, Department of Neurology, Columbia University Irving Medical Center, New York, NY, USA; Taub Institute for Research on Alzheimer's Disease and the Aging Brain, Columbia University Irving Medical Center, New York, NY, USA., de Strooper B; UK Dementia Research Institute at University College London, London, UK; Vlaams Instituut voor Biotechnologie at Katholieke Universiteit Leuven, Leuven, Belgium., Denes A; 'Momentum' Laboratory of Neuroimmunology, Institute of Experimental Medicine, Budapest, Hungary., Eggen BJL; Department of Biomedical Sciences of Cells & Systems, section Molecular Neurobiology, University of Groningen, Groningen, the Netherlands; University Medical Center Groningen, Groningen, the Netherlands., Eyo U; Department of Neuroscience, Center for Brain Immunology and Glia, University of Virginia School of Medicine, Charlottesville, VA, USA., Galea E; Institut de Neurociències and Departament de Bioquímica, Unitat de Bioquímica, Universitat Autònoma de Barcelona, Barcelona, Spain; ICREA, Barcelona, Spain., Garel S; Institut de Biologie de l'ENS (IBENS), Département de Biologie, École Normale Supérieure, CNRS, INSERM, Paris, France; College de France, Paris, France., Ginhoux F; Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A(∗)STAR), Singapore, Singapore., Glass CK; University of California San Diego School of Medicine, La Jolla, CA, USA., Gokce O; Institute for Stroke and Dementia Research, Ludwig Maximillian's University of Munich, Munich, Germany., Gomez-Nicola D; School of Biological Sciences, University of Southampton, Southampton General Hospital, Southampton, UK., González B; Unidad de Histología Medica, Depto. Biología Celular, Fisiología e Inmunología and Instituto de Neurociencias, Universidad Autónoma de Barcelona, Barcelona, Spain., Gordon S; Chang Gung University, Taoyuan City, Taiwan (ROC); Sir William Dunn School of Pathology, Oxford, UK., Graeber MB; Ken Parker Brain Tumour Research Laboratories, Brain and Mind Centre, Faculty of Medicine and Health, The University of Sydney, Camperdown, NSW, Australia., Greenhalgh AD; Lydia Becker Institute of Immunology and Inflammation, Geoffrey Jefferson Brain Research Centre, Division of Infection, Immunity & Respiratory Medicine, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester, UK., Gressens P; Université Paris Cité, Inserm, NeuroDiderot, 75019 Paris, France., Greter M; Institute of Experimental Immunology, University of Zurich, Zurich, Switzerland., Gutmann DH; Department of Neurology, Washington University School of Medicine, St. Louis, MO, USA., Haass C; Division of Metabolic Biochemistry, Faculty of Medicine, Biomedical Center (BMC), Ludwig-Maximilians-Universität Munchen, Munich, Germany; German Center for Neurodegenerative Diseases (DZNE), Munich, Germany; Munich Cluster for Systems Neurology (SyNergy); Munich, Germany., Heneka MT; Luxembourg Centre for Systems Biomedicine, University of Luxembourg, Belvaux, Luxembourg., Heppner FL; Department of Neuropathology, Charité - Universitätsmedizin Berlin, Berlin, Germany., Hong S; UK Dementia Research Institute at University College London, London, UK., Hume DA; Mater Research Institute-University of Queensland, Brisbane, QLD, Australia., Jung S; Department of Immunology and Regenerative Biology, Weizmann Institute of Science, Rehovot, Israel., Kettenmann H; Max-Delbrück Center for Molecular Medicine, Berlin, Germany; Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China., Kipnis J; Center for Brain Immunology and Glia (BIG), Department of Pathology and Immunology, Washington University in St. Louis, St. Louis, MO, USA., Koyama R; Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan., Lemke G; MNL-L, The Salk Institute for Biological Studies, La Jolla, CA, USA., Lynch M; Trinity College Institute of Neuroscience, Trinity College, Dublin, Republic of Ireland., Majewska A; Department of Neuroscience, University of Rochester, Rochester, NY, USA., Malcangio M; Wolfson Centre for Age-Related Diseases, Institute of Psychiatry, Psychology and Neuroscience, King's College London, London, UK., Malm T; University of Eastern Finland, Kuopio, Finland., Mancuso R; Microglia and Inflammation in Neurological Disorders (MIND) Lab, VIB Center for Molecular Neurology, VIB, Antwerp, Belgium; Department of Biomedical Sciences, University of Antwerp, Antwerp, Belgium., Masuda T; Department of Molecular and System Pharmacology, Graduate School of Pharmaceutical Sciences, Kyushu University, Japan., Matteoli M; Humanitas University, Department of Biomedical Sciences, Milan, Italy., McColl BW; UK Dementia Research Institute, Centre for Discovery Brain Sciences, University of Edinburgh, Edinburgh BioQuarter, Edinburgh, UK., Miron VE; MRC Centre for Reproductive Health, The Queen's Medical Research Institute, Edinburgh BioQuarter, Edinburgh, UK; UK Dementia Research Institute at the University of Edinburgh, Edinburgh BioQuarter, Edinburgh, UK., Molofsky AV; University of California, San Francisco, CA, USA., Monje M; Howard Hughes Medical Institute, (HHMI), MD, USA; Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford University, Stanford, CA, USA., Mracsko E; Roche Innovation Center, Basel, Switzerland., Nadjar A; Neurocentre Magendie, University of Bordeaux, Bordeaux, France; Institut Universitaire de France (IUF), Paris, France., Neher JJ; German Center for Neurodegenerative Diseases (DZNE), Tübingen, Germany; Department of Cellular Neurology, Hertie Institute for Clinical Brain Research, University of Tübingen, Tübingen, Germany., Neniskyte U; VU LSC-EMBL Partnership for Genome Editing Technologies, Life Sciences Center, Vilnius University, Vilnius, Lithuania; Institute of Biosciences, Life Sciences Center, Vilnius University, Vilnius, Lithuania., Neumann H; Institute of Reconstructive Neurobiology, Medical Faculty and University Hospital of Bonn, University of Bonn, Bonn, Germany., Noda M; Laboratory of Pathophysiology, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan; Institute of Mitochondrial Biology and Medicine of Xi'an Jiaotong University School of Life Science and Technology, Xi'an, China., Peng B; Department of Neurosurgery, Huashan Hospital, Institute for Translational Brain Research, State Key Laboratory of Medical Neurobiology, MOE Frontiers Center for Brain Science, Fudan University, Shanghai, China., Peri F; Department of Molecular Life Sciences, University of Zurich, Zurich, Switzerland., Perry VH; UK Dementia Research Institute, University College London, London, UK; School of Biological Sciences, University of Southampton, Southampton, UK., Popovich PG; Department of Neuroscience, College of Medicine, The Ohio State University, Columbus, OH, USA., Pridans C; University of Edinburgh, Centre for Inflammation Research, Edinburgh, UK., Priller J; Department of Psychiatry & Psychotherapy, School of Medicine, Technical University of Munich, Munich, Germany; Charité - Universitätsmedizin Berlin and DZNE, Berlin, Germany; University of Edinburgh and UK DRI, Edinburgh, UK., Prinz M; Institute of Neuropathology, Faculty of Medicine, University of Freiburg, Freiburg, Germany; Center for Basics in NeuroModulation (NeuroModulBasics), Faculty of Medicine, University of Freiburg, Freiburg, Germany; Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Freiburg, Germany., Ragozzino D; Department of Physiology and Pharmacology, Sapienza University of Rome, Rome, Italy; Santa Lucia Foundation (IRCCS Fondazione Santa Lucia), Rome, Italy., Ransohoff RM; Third Rock Ventures, Boston, MA, USA., Salter MW; Hospital for Sick Children, Toronto, ON, Canada; University of Toronto, Toronto, ON, Canada., Schaefer A; Nash Family Department of Neuroscience, Center for Glial Biology, Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA; Max Planck Institute for Biology of Ageing, Koeln, Germany., Schafer DP; Department of Neurobiology, Brudnick Neuropsychiatric Research Institute, University of Massachusetts Medical School, Worcester, MA, USA., Schwartz M; Department of Neurobiology, Weizmann Institute of Science, Rehovot, Israel., Simons M; Institute of Neuronal Cell Biology, Technical University Munich, German Center for Neurodegenerative Diseases, Munich, Germany., Smith CJ; Galvin Life Science Center, University of Notre Dame, Indianapolis, IN, USA., Streit WJ; Department of Neuroscience, University of Florida, Gainesville, FL, USA., Tay TL; Faculty of Biology, University of Freiburg, Freiburg, Germany; BrainLinks-BrainTools Centre, University of Freiburg, Freiburg, Germany; Freiburg Institute of Advanced Studies, University of Freiburg, Freiburg, Germany; Department of Biology, Boston University, Boston, MA, USA; Department of Anatomy and Neurobiology, Boston University School of Medicine, Boston, MA, USA., Tsai LH; Broad Institute of MIT and Harvard, Cambridge, MA, USA; Picower Institute for Learning and Memory, Massachusetts Institute of Technology, Cambridge, MA, USA; Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA, USA., Verkhratsky A; Achucarro Basque Center for Neuroscience, Glial Cell Biology Lab, Leioa, Spain; Department of Neuroscience, University of the Basque Country EHU/UPV, Leioa, Spain; Faculty of Biology, Medicine and Health, The University of Manchester, Manchester, UK., von Bernhardi R; Faculty of Medicine and Science, Universidad San Sebastian, Santiago, Chile., Wake H; Department of Anatomy and Molecular Cell Biology, Graduate School of Medicine, Nagoya University, Nagoya, Japan., Wittamer V; Institut de Recherche Interdisciplinaire en Biologie Humaine et Moléculaire (IRIBHM), Université Libre de Bruxelles (ULB), Brussels, Belgium; ULB Neuroscience Institute (UNI), Université Libre de Bruxelles (ULB), Brussels, Belgium., Wolf SA; Charité Universitätsmedizin, Experimental Ophthalmology and Neuroimmunology, Berlin, Germany., Wu LJ; Department of Neurology and Department of Immunology, Mayo Clinic, Rochester, MN, USA., Wyss-Coray T; Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford University, Stanford, CA, USA.

Publikováno v: Neuron [Neuron] 2022 Nov 02; Vol. 110 (21), pp. 3458-3483.