Výsledky vyhledávání

Gene tagging and gene replacement using recombinase-mediated cassette exchange in Schizosaccharomyces pombe

Autor: Adam T. Watson, Valerie Garcia, Antony M. Carr, John Armstrong, Neil Bone

Publikováno v: Gene. 407:63-74

Cre/lox site-specific recombination systems provide important tools for genetic manipulation. Here we present an efficient method for gene tagging and gene replacement using Cre recombinase-mediated cassette exchange (RMCE). The cassette consists of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::12f3ee3d3fc06e2238cbc108f42b3e48
https://doi.org/10.1016/j.gene.2007.09.024

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The role and aims of the FYSSION project

Autor: Neil Bone, Timothy Beck, James Dodgson, John Armstrong

Publikováno v: Briefings in Functional Genomics and Proteomics. 6:3-7

FYSSION is a resource for researchers working on the fission yeast Schizosaccharomyces pombe. It currently comprises libraries of temperature-sensitive mutants in essential genes, and insertional mutants in non-essential genes, available for screenin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f3d0d3e46e80d6faf8113a5fad9ed8ee
https://doi.org/10.1093/bfgp/elm004

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Polar Assembly in a Designed Protein Fiber

Autor: Andrew M. Smith, Maxim G. Ryadnov, Marryat S. P. Stevens, Steve F. A. Acquah, David R. M. Walton, Harold W. Kroto, Derek N. Woolfson, Neil Bone

Publikováno v: Angewandte Chemie International Edition. 44:325-328

Bioinspiration: Polar assembly (a feature of many natural protein-based fibrous structures, such as actin, microtubules, intermediate filaments, and collagens) is demonstrated in a straightforward, synthetic, peptide-based fiber system of de novo des

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3f3c8fa0662d62da1eb678a1cea0d21b
https://doi.org/10.1002/anie.200461599

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Elektronická kniha

Observing Meteors, Comets, Supernovae and Other Transient Phenomena

Autor: Neil Bone

Many books cover permanent astronomical objects, but few consider transient, out-of-the-ordinary astronomical phenomena. This book covers a wide range of topics, from the partly-predictable to the totally unpredictable. At one end: of the scale, it g

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Solution Characterisation by NMR Spectroscopy of Two Horseradish Peroxidase Isoenzyme C Mutants with Alanine Replacing Either Phe142 or Phe143

Autor: Andrew T. Smith, Nigel C. Veitch, Julian F. Burke, Robert J. P. Williams, Neil Bone

Publikováno v: European Journal of Biochemistry. 233:650-658

Site-directed mutagenesis of the horseradish peroxidase isoenzyme C (HRP C) gene has been undertaken in order to provide two recombinant enzymes where alanine replaces either Phe142 or Phe143 ([F142A]HRP C and [F143A]HRP C, respectively). These heme

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d03009e67d20728a4ab12b504ead2fc8
https://doi.org/10.1111/j.1432-1033.1995.650_2.x

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The ypt proteins of Schizosaccharomyces pombe

Autor: Sally Bowden, Mark Craighead, John Armstrong, Alison Pidoux, Neil Bone, Everton Robinson

Publikováno v: Biochemical Society Transactions. 22:460-463

65 66 Mayer, B. J., Ren, K., Clark, K. I,. and Baltimore, D. (1993) Cell 73,629-630 Haslarn, K. J., Koide, H. B. and Hemmings, B. A. (1993) Nature (London) 363,309-310 Musacchio, A., Gibson, T., Price, P., Thompson, J. and Saraste, M. (1993) Trends B

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::64a646bb019923f7fc8e893de28be21c
https://doi.org/10.1042/bst0220460

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