Zobrazeno 1 - 10
of 22
pro vyhledávání: '"Nehad Hirmiz"'
Autor:
James M Pemberton, Dang Nguyen, Elizabeth J Osterlund, Wiebke Schormann, Justin P Pogmore, Nehad Hirmiz, Brian Leber, David W Andrews
Publikováno v:
eLife, Vol 12 (2023)
Anti-apoptotic proteins such as BCL-XL promote cell survival by sequestering pro-apoptotic BCL-2 family members, an activity that frequently contributes to tumorigenesis. Thus, the development of small-molecule inhibitors for anti-apoptotic proteins,
Externí odkaz:
https://doaj.org/article/c46858294f8f416e96855c98bce4cf03
Publikováno v:
Membranes, Vol 5, Iss 4, Pp 702-721 (2015)
Diffusion in lipid membranes is an essential component of many cellular process and fluorescence a method of choice to study membrane dynamics. The goal of this work was to directly compare two common fluorescence methods, line-scanning fluorescence
Externí odkaz:
https://doaj.org/article/c5248f69b62e4d84a0451758ef53f5b3
Publikováno v:
Photonics, Vol 3, Iss 4, p 56 (2016)
Current instruments used to detect specific protein-protein interactions in live cells for applications in high-content screening (HCS) are limited by the time required to measure the lifetime. Here, a 32 × 1 single-photon avalanche diode (SPAD) arr
Externí odkaz:
https://doaj.org/article/0c97f707bf524ea4b9d9cfe8b78f2a37
Autor:
Nehad Hirmiz, Elizabeth J Osterlund, Morgan Richards, David W. Andrews, Qiyin Fang, Anthony Tsikouras
Publikováno v:
IEEE Journal of Selected Topics in Quantum Electronics. 27:1-9
Protein-protein interactions can be measured in live cells, at nanometer scale, using Fluorescence Lifetime Imaging Microscopy (FLIM) enabled Forster Resonance Energy Transfer (FRET). There are growing interests in exploring protein-protein interacti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::173a48143a9029e3ea1ef2ef0559be55
https://doi.org/10.1109/jstqe.2020.2997834
https://doi.org/10.1109/jstqe.2020.2997834
Fluorescence lifetime imaging microscopy (FLIM) can detect Fӧrster resonance energy transfer (FRET) to measure protein-protein interactions in live cells. Traditional FLIM-FRET uses relative intensities to represent protein expression, consequently
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3882eb55242b985bb0f537024ad6625c
https://doi.org/10.21203/rs.3.pex-1354/v1
https://doi.org/10.21203/rs.3.pex-1354/v1
Autor:
Elizabeth J. Osterlund, Nehad Hirmiz, James M. Pemberton, Adrien Nougarède, Qian Liu, Brian Leber, Qiyin Fang, David W. Andrews
Publikováno v:
Science Advances. 8
Cytoplasmic and membrane-bound BCL-2 family proteins regulate apoptosis, a form of programmed cell death, via dozens of binary protein interactions confounding measurement of the effects of inhibitors in live cells. In cancer, apoptosis is frequently
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7693edb79c1d1712aef49cd51ea6871a
https://doi.org/10.1126/sciadv.abm7375
https://doi.org/10.1126/sciadv.abm7375
Autor:
Elizabeth J. Osterlund, Nehad Hirmiz, Dang Nguyen, James M. Pemberton, Qiyin Fang, David W. Andrews
Publikováno v:
Journal of Biological Chemistry. 299:102863
The pro-apoptotic BH3-only endoplasmic reticulum (ER) resident protein BIK, positively regulates mitochondrial outer membrane permeabilization (MOMP), the point-of-no-return in apoptosis. It is generally accepted that BIK functions at a distance from
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2e8c1ad2b2eaba6938b9f3607b4d6555
https://doi.org/10.1016/j.jbc.2022.102863
https://doi.org/10.1016/j.jbc.2022.102863
Autor:
Anthony Tsikouras, Qiyin Fang, Nehad Hirmiz, David W. Andrews, Elizabeth J Osterlund, Morgan Richards
Publikováno v:
Optics express. 27(16)
The streak camera is a picosecond resolution photodetector with parallel input capability; however, the degree of multiplexing is limited by crosstalk and temporal uncertainty in the sweeping field. We introduced a fixed time delay between adjacent f
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5d7ae9fa6ef3fb3c5e600170468ec99b
https://pubmed.ncbi.nlm.nih.gov/31510548
https://pubmed.ncbi.nlm.nih.gov/31510548
Autor:
Elizabeth J Osterlund, Nehad Hirmiz, Qiyin Fang, Anthony Tsikouras, Jessica Kun, Morgan Richards, David W. Andrews
Publikováno v:
Advances in Microscopic Imaging II.
Quantitative measurement of protein-protein interaction is important for many biological processes, including cell growth, intercellular communication, gene expression and apoptosis. Forster Resonance Energy Transfer (FRET) provides a molecular level
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::721ababb6bb4086bf64b073876427f09
https://doi.org/10.1117/12.2526906
https://doi.org/10.1117/12.2526906