Rapid MinION profiling of preterm microbiota and antimicrobial-resistant pathogens

Autor: Magdalena Kujawska, Paul Clarke, Shabhonam Caim, Thomas C. Brook, Darren Heavens, Ned Peel, Lesley Hoyles, Holly Acford-Palmer, Samuel Martin, Cristina Alcon-Giner, Lindsay J. Hall, Matthew D. Clark, Richard M. Leggett

Publikováno v: Nature Microbiology

The MinION sequencing platform offers near real-time analysis of DNA sequence; this makes the tool attractive for deployment in fieldwork or clinical settings. We used the MinION platform coupled to the NanoOK RT software package to perform shotgun m

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::52de81426f528bb21fc622f0fceb03a8
https://doi.org/10.1038/s41564-019-0626-z

Meiosis and beyond – understanding the mechanistic and evolutionary processes shaping the germline genome

Autor: Lee Larcombe, Alexander Suh, Yu Sun, Simone Immler, Mette M. Mogensen, Matthew J. Neale, Rebecca E. O’Connor, Ben Skinner, Peter J. I. Ellis, Claudia Cattoni Rathje, Raheleh Rahbari, Alison C Tidy, Ned Peel, D. S. Murray, Iain C. Macaulay, Christian S. Ottolini, Will Nash, Wilfried Haerty, Michael C. Summers, Tarang K. Mehta, Roberta Bergero, Luke Ramsey

Publikováno v: Biological Reviews of the Cambridge Philosophical Society
Bergero, R, Ellis, P, Haerty, W, Larcombe, L, Macaulay, I, Mehta, T, Mogensen, M, Murray, D, Nash, W, Neale, M J, O'Connor, R, Ottolini, C, Peel, N, Ramsey, L, Skinner, B, Suh, A, Summers, M, Sun, Y, Tidy, A, Rahbari, R, Rathje, C & Immler, S 2021, ' Meiosis and beyond – understanding the mechanistic and evolutionary processes shaping the germline genome ', Biological reviews, vol. 96, no. 3, pp. 822-841 . https://doi.org/10.1111/brv.12680

The separation of germ cell populations from the soma is part of the evolutionary transition to multicellularity. Only genetic information present in the germ cells will be inherited by future generations, and any molecular processes affecting the ge

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5013a65174293a5982c544ce7554251c

Metagenomic analysis of planktonic riverine microbial consortia using nanopore sequencing reveals insight into river microbe taxonomy and function

Autor: Terrance P. Snutch, Spyridon Oikonomopoulos, Tue Kjærgaard Nielsen, Miten Jain, Eric van der Helm, Richard M. Leggett, Dino Jolic, Hollian Richardson, Justin O'Grady, Hans J. Jansen, Jiannis Ragoussis, Ned Peel, David Eccles, Devin M. Drown, Lars Hestbjerg Hansen, Thomas Barry, John R. Tyson, Anne-Lise Ducluzeau, Bonnie L. Brown, Anthony Bayega, Kate Reddington, Darren Heavens

Publikováno v: Reddington, K, Eccles, D, O'Grady, J, Drown, D M, Hansen, L H, Nielsen, T K, Ducluzeau, A L, Leggett, R M, Heavens, D, Peel, N, Snutch, T P, Bayega, A, Oikonomopoulos, S, Ragoussis, J, Barry, T, van der Helm, E, Jolic, D, Richardson, H, Jansen, H, Tyson, J R, Jain, M & Brown, B L 2020, ' Metagenomic analysis of planktonic riverine microbial consortia using nanopore sequencing reveals insight into river microbe taxonomy and function ', GigaScience, vol. 9, no. 6, giaa053 . https://doi.org/10.1093/gigascience/giaa053
Reddington, K, Eccles, D, O'Grady, J, Drown, D M, Hansen, L H, Nielsen, T K, Ducluzeau, A-L, Leggett, R M, Heavens, D, Peel, N, Snutch, T P, Bayega, A, Oikonomopoulos, S, Ragoussis, J, Barry, T, van der Helm, E, Jolic, D, Richardson, H, Jansen, H, Tyson, J R, Jain, M & Brown, B L 2020, ' Metagenomic analysis of planktonic riverine microbial consortia using nanopore sequencing reveals insight into river microbe taxonomy and function ', GigaScience, vol. 9, no. 6, 053 . https://doi.org/10.1093/gigascience/giaa053
Reddington, K, Eccles, D, O'Grady, J, Drown, D M, Hansen, L H, Nielsen, T K, Ducluzeau, A L, Leggett, R M, Heavens, D, Peel, N, Snutch, T P, Bayega, A, Oikonomopoulos, S, Ragoussis, I, Barry, T, van der Helm, E, Jolic, D, Richardson, H, Jansen, H, Tyson, J R, Jain, M & Brown, B L 2020, ' Metagenomic analysis of planktonic riverine microbial consortia using nanopore sequencing reveals insight into river microbe taxonomy and function ', GigaScience, vol. 9, no. 6, giaa053 . https://doi.org/10.1093/gigascience/giaa053

Background Riverine ecosystems are biogeochemical powerhouses driven largely by microbial communities that inhabit water columns and sediments. Because rivers are used extensively for anthropogenic purposes (drinking water, recreation, agriculture, a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9cb7f41189bf05d654da37b7b0fa9ab2
https://orbit.dtu.dk/en/publications/503cff13-37a3-40e7-ac13-683a2ac694b3

Semi-quantitative characterisation of mixed pollen samples using MinION sequencing and Reverse Metagenomics (RevMet)

Autor: Lawrence Percival-Alwyn, Douglas W. Yu, Darren Heavens, Lynn V. Dicks, Richard G. Davies, Richard M. Leggett, Matthew D. Clark, Ned Peel, Christopher B. Cooper

1. The ability to identify and quantify the constituent plant species that make up a mixed-species sample of pollen has important applications in ecology, conservation, and agriculture. Recently, metabarcoding protocols have been developed for pollen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::438eaf0ea948a34423acbc77251fb459

Collection and purification of river water samples v1

Autor: Kate Reddington, David Eccles, Justin O’Grady, Devin M. Drown, Lars Hestbjerg Hansen, Tue Kjærgaard Nielsen, Anne-Lise Ducluzeau, Richard M. Leggett, Darren Heavens, Ned Peel, Terrance P. Snutch, Anthony Bayega, Spyros Oikonomopoulos, Jiannis Ragoussis, Thomas Barry, Eric van der Helm, Dino Jolic, Hollian Richardson, Hans Jansen, John R. Tyson, Miten Jain, Bonnie L. Brown

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::c744d75892f9c9b651d97c83c85b4cba
https://doi.org/10.17504/protocols.io.qsydwfw