A pooled CRISPR/AsCpf1 screen using paired gRNAs to induce genomic deletions in Chinese hamster ovary cells

Autor: Valerie Schmieder, Neža Novak, Heena Dhiman, Ly Ngoc Nguyen, Evgenija Serafimova, Gerald Klanert, Martina Baumann, Helene Faustrup Kildegaard, Nicole Borth

Publikováno v: Biotechnology Reports, Vol 31, Iss , Pp e00649- (2021)

Chinese hamster ovary (CHO) cells are the most widely used host for the expression of therapeutic proteins. Recently, significant progress has been made due to advances in genome sequence and annotation quality to unravel the black box CHO. Neverthel

Externí odkaz: https://doaj.org/article/12a1be4ab7124a27af33fa7b29cc47d4

The contribution of Slovenian biogas plants to the reduction of agricultural sector green house emissions

Autor: Romana MARINŠEK LOGAR, Neža NOVAK, Maša VODOVNIK

Publikováno v: Acta Agriculturae Slovenica, Vol 106, Iss 1 (2015)

Agriculture is a source of emissions of the greenhouse gas methane into the environment. These emissions can be reduced by appropriate storage of animal slurry and manure, with proper fertilization and processing of organic agricultural waste into bi

Externí odkaz: https://doaj.org/article/1a70f3bbbc0b430e84771799a85fbb75

A pooled CRISPR/AsCpf1 screen using paired gRNAs to induce genomic deletions in Chinese hamster ovary cells

Autor: Gerald Klanert, Neža Novak, Heena Dhiman, Evgenija Serafimova, Valerie Schmieder, Helene Faustrup Kildegaard, Ly Ngoc Nguyen, Martina Baumann, Nicole Borth

Publikováno v: Schmieder, V, Novak, N, Dhiman, H, Nguyen, L N, Serafimova, E, Klanert, G, Baumann, M, Kildegaard, H F & Borth, N 2021, ' A pooled CRISPR/AsCpf1 screen using paired gRNAs to induce genomic deletions in Chinese hamster ovary cells ', Biotechnology Reports, vol. 31, e00649 . https://doi.org/10.1016/j.btre.2021.e00649
Biotechnology Reports, Vol 31, Iss, Pp e00649-(2021)
Biotechnology Reports

Highlights • Development of a small-scale CRISPR/AsCpf1 screen in CHO. • Usage of paired gRNAs enables full deletion of coding or noncoding genomic regions. • Growth perturbing paired gRNAs identified. • Key points for considerations in futur

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d31d246764716135df37256ce4e0360d
https://orbit.dtu.dk/en/publications/2952ac8e-767c-4605-811b-710e6cd4ba19

Targeting Malignant Brain Tumors with Antibodies

Autor: Vladka Čurin Šerbec, Rok Razpotnik, Uros Rajcevic, Neža Novak

Publikováno v: Frontiers in Immunology
Frontiers in Immunology, Vol 8 (2017)

Antibodies have been shown to be a potent therapeutic tool. However, their use for targeting brain diseases, including neurodegenerative diseases and brain cancers, has been limited, particularly because the blood-brain barrier (BBB) makes brain tiss

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a05cae488aed301d6cb69a702e5cf248
http://europepmc.org/articles/PMC5622144