Zobrazeno 1 - 10 of 85 pro vyhledávání: '"Natsuko, Miura"'
1
Akademický článek
Mutualistic Interactions between Dinoflagellates and Pigmented Bacteria Mitigate Environmental Stress
Autor: Toshiyuki Takagi, Kako Aoyama, Keisuke Motone, Shunsuke Aburaya, Hideyuki Yamashiro, Natsuko Miura, Koji Inoue
Publikováno v: Microbiology Spectrum, Vol 11, Iss 1 (2023)
ABSTRACT Scleractinian corals form symbiotic relationships with a variety of microorganisms, including endosymbiotic dinoflagellates of the family Symbiodiniaceae, and with bacteria, which are collectively termed coral holobionts. Interactions betwee
Externí odkaz: https://doaj.org/article/ea993b76e8f944418860d72622cf5666
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2
Akademický článek
Foci-forming regions of pyruvate kinase and enolase at the molecular surface incorporate proteins into yeast cytoplasmic metabolic enzymes transiently assembling (META) bodies.
Autor: Ryotaro Utsumi, Yuki Murata, Sayoko Ito-Harashima, Misaki Akai, Natsuko Miura, Kouichi Kuroda, Mitsuyoshi Ueda, Michihiko Kataoka
Publikováno v: PLoS ONE, Vol 18, Iss 4, p e0283002 (2023)
Spatial reorganization of metabolic enzymes to form the "metabolic enzymes transiently assembling (META) body" is increasingly recognized as a mechanism contributing to regulation of cellular metabolism in response to environmental changes. A number
Externí odkaz: https://doaj.org/article/b0b937c83a3742d7aff019edfb424362
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3
Akademický článek
Peptide barcoding for one-pot evaluation of sequence–function relationships of nanobodies
Autor: Yusei Matsuzaki, Wataru Aoki, Takumi Miyazaki, Shunsuke Aburaya, Yuta Ohtani, Kaho Kajiwara, Naoki Koike, Hiroyoshi Minakuchi, Natsuko Miura, Tetsuya Kadonosono, Mitsuyoshi Ueda
Publikováno v: Scientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
Abstract Optimisation of protein binders relies on laborious screening processes. Investigation of sequence–function relationships of protein binders is particularly slow, since mutants are purified and evaluated individually. Here we developed pep
Externí odkaz: https://doaj.org/article/6ca696081ad148fc8e2a41f6d690936f
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4
Akademický článek
High-throughput identification of peptide agonists against GPCRs by co-culture of mammalian reporter cells and peptide-secreting yeast cells using droplet microfluidics
Autor: Kenshi Yaginuma, Wataru Aoki, Natsuko Miura, Yuta Ohtani, Shunsuke Aburaya, Masato Kogawa, Yohei Nishikawa, Masahito Hosokawa, Haruko Takeyama, Mitsuyoshi Ueda
Publikováno v: Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019)
Abstract Since G-protein coupled receptors (GPCRs) are linked to various diseases, screening of functional ligands against GPCRs is vital for drug discovery. In the present study, we developed a high-throughput functional cell-based assay by combinin
Externí odkaz: https://doaj.org/article/e4aeabe5ce914f16bea38ae6cd9902d6
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5
Akademický článek
Domain swapping of complementarity-determining region in nanobodies produced by Pichia pastoris
Autor: Natsuko Miura, Kana Miyamoto, Yuta Ohtani, Kenshi Yaginuma, Shunsuke Aburaya, Yoshinori Kitagawa, Wataru Aoki, Mitsuyoshi Ueda
Publikováno v: AMB Express, Vol 9, Iss 1, Pp 1-8 (2019)
Abstract Easy preparation of chimeric nanobodies with various scaffolds is important for customizing abilities of nanobodies toward practical utilization. To accomplish high-throughput production of various nanobodies, utilization of microbes is an a
Externí odkaz: https://doaj.org/article/24cfb688985b4ad3a517218935a8f161
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7
Akademický článek
A Zeaxanthin-Producing Bacterium Isolated from the Algal Phycosphere Protects Coral Endosymbionts from Environmental Stress
Autor: Keisuke Motone, Toshiyuki Takagi, Shunsuke Aburaya, Natsuko Miura, Wataru Aoki, Mitsuyoshi Ueda
Publikováno v: mBio, Vol 11, Iss 1 (2020)
ABSTRACT Reef-building corals form a complex consortium with photosynthetic algae in the family Symbiodiniaceae and bacteria, collectively termed the coral holobiont. These bacteria are hypothesized to be involved in the stress resistance of the cora
Externí odkaz: https://doaj.org/article/3c0a3b91b882442787fbcf3aff569c32
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8
Akademický článek
Condensate Formation by Metabolic Enzymes in Saccharomyces cerevisiae
Autor: Natsuko Miura
Publikováno v: Microorganisms, Vol 10, Iss 2, p 232 (2022)
Condensate formation by a group of metabolic enzymes in the cell is an efficient way of regulating cell metabolism through the formation of “membrane-less organelles.” Because of the use of green fluorescent protein (GFP) for investigating protei
Externí odkaz: https://doaj.org/article/8d47f53fa2cf43fa8a13c4f31de757c4
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9
Akademický článek
Peptide barcoding for establishment of new types of genotype-phenotype linkages.
Autor: Kana Miyamoto, Wataru Aoki, Yuta Ohtani, Natsuko Miura, Shunsuke Aburaya, Yusei Matsuzaki, Kaho Kajiwara, Yoshinori Kitagawa, Mitsuyoshi Ueda
Publikováno v: PLoS ONE, Vol 14, Iss 4, p e0215993 (2019)
Measuring binding properties of binders (e.g., antibodies) is essential for developing useful experimental reagents, diagnostics, and pharmaceuticals. Display technologies can evaluate a large number of binders in a high-throughput manner, but the im
Externí odkaz: https://doaj.org/article/c0de412af4f444c3a965f543cc618835
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