Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Nathaniel D, Graham"'
Publikováno v:
Plant Direct, Vol 4, Iss 3, Pp n/a-n/a (2020)
Abstract Site‐specific recombinase enzymes function in heterologous cellular environments to initiate strand‐switching reactions between unique DNA sequences termed recombinase binding sites. Depending on binding site position and orientation, re
Externí odkaz:
https://doaj.org/article/98250771f54b48d79e75bc3faadca90f
Autor:
Jon P. Cody, Nathan C. Swyers, Morgan E. McCaw, Nathaniel D. Graham, Changzeng Zhao, James A. Birchler
Publikováno v:
Agronomy, Vol 5, Iss 3, Pp 309-321 (2015)
Minichromosome technology has the potential to offer a number of possibilities for expanding current biofortification strategies. While conventional genome manipulations rely on random integration of one or a few genes, engineered minichromosomes wou
Externí odkaz:
https://doaj.org/article/bf928fdc086a4d8eb2685a0e73e6b8bd
Autor:
Peter L. Morrell, Robert M. Stupar, Miguel E. Vega-Sanchez, David M. Bubeck, John A. Lindbo, Kevin C. Glenn, Raymond C. Dobert, Gunvant Patil, Annie T. Gutsche, Sandeep Kumar, Gregory D. May, Nathaniel D. Graham, Luis Maas
Publikováno v:
Plant Physiol
Site-directed nucleases (SDNs) used for targeted genome editing are powerful new tools to introduce precise genetic changes into plants. Like traditional approaches, such as conventional crossing and induced mutagenesis, genome editing aims to improv
Autor:
Nathaniel D. Graham, David G. Schwark, Thomas J. Poorten, Fräbel Sabine, Jingyi Nie, Shai J. Lawit
Publikováno v:
Genome editing for precision crop breeding ISBN: 9781786764478
Genome editing for precision crop breeding
Genome editing for precision crop breeding
Base editors are gene editing tools that allow targeted nucleic acid conversions, most commonly C>T and A>G, through pairing of deamination domains with impaired nucleases. Multiple deaminase domains and architectures have been demonstrated in planta
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::206394d5104eaf6e1a3ff1e7e16d8e60
https://doi.org/10.1201/9781003048237-6
https://doi.org/10.1201/9781003048237-6
Publikováno v:
Current protocols in plant biologyLiterature cited. 2(4)
Binary Bacterial Artificial Chromosomes (BiBAC) are large insert cloning vectors that contain the necessary features required for Agrobacterium-mediated transformation. However, the large size of BiBACs and low-copy number in Escherichia coli (DH10B)
Autor:
Nathan C. Swyers, Morgan E. McCaw, James A. Birchler, Jon P. Cody, Changzeng Zhao, Nathaniel D. Graham
Publikováno v:
Current Protocols in Plant Biology
Fluorescence In Situ Hybridization (FISH) is the annealing of fluorescent DNA probes to their complementary sequences on prepared chromosomes and subsequent visualization with a fluorescent microscope. In maize, FISH is useful for distinguishing each
Autor:
Nathan C. Swyers, Jon P. Cody, James A. Birchler, Morgan E. McCaw, Nathaniel D. Graham, Changzeng Zhao
Publikováno v:
Current Protocols in Plant Biology
High-quality preparations of chromosomes are useful for many purposes. To prepare metaphase chromosome spreads in maize, root tips are harvested and treated with nitrous oxide to stop cell division at metaphase before being fixed in acetic acid. This
Autor:
Jon P. Cody, Robert T. Gaeta, Morgan E. McCaw, Nathan C. Swyers, Changzeng Zhao, James A. Birchler, Nathaniel D. Graham
Publikováno v:
Current Protocols in Plant Biology
Minichromosomes have been generated in maize using telomere-mediated truncation. Telomere DNA, because of its repetitive nature, can be difficult to manipulate. The protocols in this unit describe two methods for generating the telomere DNA required
Publikováno v:
Current Opinion in Biotechnology. 37:135-142
Plant minichromosomes have the potential for stacking multiple traits on a separate entity from the remainder of the genome. Transgenes carried on an independent chromosome would facilitate conferring many new properties to plants and using minichrom
Autor:
Changzeng Zhao, James A. Birchler, Jon P. Cody, Nathan C. Swyers, Nathaniel D. Graham, Morgan E. McCaw
Publikováno v:
Methods in Molecular Biology ISBN: 9781493949298
Artificial minichromosomes are non-integrating vectors capable of stably maintaining transgenes outside of the main chromosome set. The production of minichromosomes relies on telomere-mediated chromosomal truncation, which involves introducing trans
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4c36604232a73cc08b0e0dfa5c13ac7c
https://doi.org/10.1007/978-1-4939-4931-1_1
https://doi.org/10.1007/978-1-4939-4931-1_1