Zobrazeno 1 - 10
of 23
pro vyhledávání: '"Nathan E. Thomas"'
Autor:
Peyton J. Spreacker, Nathan E. Thomas, Will F. Beeninga, Merissa Brousseau, Colin J. Porter, Kylie M. Hibbs, Katherine A. Henzler-Wildman
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-12 (2022)
Antibiotic resistance through multidrug efflux is a major hurdle in antibiotic development. Here, authors experimentally demonstrate that EmrE, a small multidrug resistance efflux pump from E. coli, can confer resistance or susceptibility depending o
Externí odkaz:
https://doaj.org/article/78d3e70700c04c05bf5b159674cdf840
Autor:
Alexander A. Shcherbakov, Grant Hisao, Venkata S. Mandala, Nathan E. Thomas, Mohammad Soltani, E. A. Salter, James H. Davis, Katherine A. Henzler-Wildman, Mei Hong
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
The small proton-coupled transporter EmrE confers multidrug resistance in bacteria. The structure of drug-bound EmrE in phospholipid bilayers is now determined using solid-state NMR. The structure provides detailed insights into the molecular mechani
Externí odkaz:
https://doaj.org/article/db0829dbaea348d0bf08d38e1f44870d
Autor:
Katarzyna Potrykus, Nathan E. Thomas, Bożena Bruhn-Olszewska, Michał Sobala, Maciej Dylewski, Tamara James, Michael Cashel
Publikováno v:
Frontiers in Microbiology, Vol 11 (2020)
The Mesh1 class of hydrolases found in bacteria, metazoans and humans was discovered as able to cleave an intact pyrophosphate residue esterified on the 3′hydroxyl of (p)ppGpp in a Mn2+ dependent reaction. Here, thin layer chromatography (TLC) qual
Externí odkaz:
https://doaj.org/article/6ce7aa90bc7e491cb21d7576de2d686e
Publikováno v:
Biophysical Journal. 122:400a
Publikováno v:
The Journal of Biological Chemistry
Transport stoichiometry determination can provide great insight into the mechanism and function of ion-coupled transporters. Traditional reversal potential assays are a reliable, general method for determining the transport stoichiometry of ion-coupl
Autor:
Nathan E. Thomas, Emma A. Morrison, Anne E. Robinson, Josephine P. Werner, Chao Wu, Katherine A. Henzler-Wildman
Publikováno v:
Journal of Biological Chemistry. 293:19137-19147
Ion-coupled transporters must regulate access of ions and substrates into and out of the binding site to actively transport substrates and minimize dissipative leak of ions. Within the single-site alternating access model, competitive substrate bindi
Autor:
Mei Hong, E. A. Salter, Mohammad Soltani, Katherine A. Henzler-Wildman, Nathan E. Thomas, Venkata S. Mandala, Alexander A. Shcherbakov, James H. Davis, Grant Hisao
Publikováno v:
Nature Communications
Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
The dimeric transporter, EmrE, effluxes polyaromatic cationic drugs in a proton-coupled manner to confer multidrug resistance in bacteria. Although the protein is known to adopt an antiparallel asymmetric topology, its high-resolution drug-bound stru
Transport stoichiometry provides insight into the mechanism and function of ion-coupled transporters, but measuring transport stoichiometry is time-consuming and technically difficult. With the increasing evidence that many ion-coupled transporters e
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::caf1048796f56eba7773b432e24c3f4a
Publikováno v:
The FASEB Journal. 33
Autor:
David L. Swanson, Nathan E. Thomas
Publikováno v:
Journal of Avian Biology. 50