Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Nathan C. Swyers"'
Publikováno v:
Plant Direct, Vol 4, Iss 3, Pp n/a-n/a (2020)
Abstract Site‐specific recombinase enzymes function in heterologous cellular environments to initiate strand‐switching reactions between unique DNA sequences termed recombinase binding sites. Depending on binding site position and orientation, re
Externí odkaz:
https://doaj.org/article/98250771f54b48d79e75bc3faadca90f
Autor:
Fangpu Han, Jonathan C. Lamb, Morgan E. McCaw, Zhi Gao, Bing Zhang, Nathan C. Swyers, James A. Birchler
Publikováno v:
Frontiers in Plant Science, Vol 9 (2018)
Multiple centromere misdivision derivatives of a translocation between the supernumerary B chromosome and the short arm of chromosome 9 (TB-9Sb) permit investigation of how centromeres of different sizes behave in meiosis in opposition or in competit
Externí odkaz:
https://doaj.org/article/09a2b09935ca44cb86b78a2a378d83d1
Autor:
Jon P. Cody, Nathan C. Swyers, Morgan E. McCaw, Nathaniel D. Graham, Changzeng Zhao, James A. Birchler
Publikováno v:
Agronomy, Vol 5, Iss 3, Pp 309-321 (2015)
Minichromosome technology has the potential to offer a number of possibilities for expanding current biofortification strategies. While conventional genome manipulations rely on random integration of one or a few genes, engineered minichromosomes wou
Externí odkaz:
https://doaj.org/article/bf928fdc086a4d8eb2685a0e73e6b8bd
Autor:
Nathan C. Swyers, Morgan E. McCaw, James A. Birchler, Jon P. Cody, Changzeng Zhao, Nathaniel D. Graham
Publikováno v:
Current Protocols in Plant Biology
Fluorescence In Situ Hybridization (FISH) is the annealing of fluorescent DNA probes to their complementary sequences on prepared chromosomes and subsequent visualization with a fluorescent microscope. In maize, FISH is useful for distinguishing each
Autor:
Nathan C. Swyers, Jon P. Cody, James A. Birchler, Morgan E. McCaw, Nathaniel D. Graham, Changzeng Zhao
Publikováno v:
Current Protocols in Plant Biology
High-quality preparations of chromosomes are useful for many purposes. To prepare metaphase chromosome spreads in maize, root tips are harvested and treated with nitrous oxide to stop cell division at metaphase before being fixed in acetic acid. This
Autor:
Jon P. Cody, Robert T. Gaeta, Morgan E. McCaw, Nathan C. Swyers, Changzeng Zhao, James A. Birchler, Nathaniel D. Graham
Publikováno v:
Current Protocols in Plant Biology
Minichromosomes have been generated in maize using telomere-mediated truncation. Telomere DNA, because of its repetitive nature, can be difficult to manipulate. The protocols in this unit describe two methods for generating the telomere DNA required
Autor:
Nathan C. Swyers, James A. Birchler
Publikováno v:
Experimental cell research. 388(2)
Artificial chromosome platforms are described in plants. Because the function of centromeres is largely epigenetic, attempts to produce artificial chromosomes with plant centromere DNA have failed. The removal of the centromeric sequences from the ce
Publikováno v:
Current Opinion in Biotechnology. 37:135-142
Plant minichromosomes have the potential for stacking multiple traits on a separate entity from the remainder of the genome. Transgenes carried on an independent chromosome would facilitate conferring many new properties to plants and using minichrom
Autor:
Changzeng Zhao, James A. Birchler, Jon P. Cody, Nathan C. Swyers, Nathaniel D. Graham, Morgan E. McCaw
Publikováno v:
Methods in Molecular Biology ISBN: 9781493949298
Artificial minichromosomes are non-integrating vectors capable of stably maintaining transgenes outside of the main chromosome set. The production of minichromosomes relies on telomere-mediated chromosomal truncation, which involves introducing trans
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4c36604232a73cc08b0e0dfa5c13ac7c
https://doi.org/10.1007/978-1-4939-4931-1_1
https://doi.org/10.1007/978-1-4939-4931-1_1
Autor:
Nathaniel D, Graham, Jon P, Cody, Nathan C, Swyers, Morgan E, McCaw, Changzeng, Zhao, James A, Birchler
Publikováno v:
International review of cell and molecular biology. 318
Engineered minichromosomes are small chromosomes that contain a transgene and selectable marker, as well as all of the necessary components required for maintenance in an organism separately from the standard chromosome set. The separation from endog