Revealing Additional Stereocomplementary Pairs of Old Yellow Enzymes by Rational Transfer of Engineered Residues

Autor: Sabine Duewel, Nathalie Nett, Sabrina Hoebenreich, Alexandra A. Richter

Publikováno v: ChemBioChem. 18:685-691

Every year numerous protein engineering and directed evolution studies are published, increasing the knowledge that could be used by protein engineers. Here we test a protein engineering strategy that allows quick access to improved biocatalysts with

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dec823240ab6754f5c7078f5b19e7154
https://doi.org/10.1002/cbic.201600688

P450-Catalyzed Regio- and Diastereoselective Steroid Hydroxylation: Efficient Directed Evolution Enabled by Mutability Landscaping

Autor: Richard Lonsdale, Kirsty J. McLean, Charles G. Gamble, Yuval Nov, Christophe Farès, Aitao Li, Julia B. Lingnau, Adrian J. Mulholland, Manfred T. Reetz, David Leys, Heike Hinrichs, Alfred Deege, Sabrina Hoebenreich, Carlos G. Acevedo-Rocha, Nathalie Nett, Cornelia Wirtz, Andrew W. Munro

Publikováno v: Acevedo-Rocha, C G, Gamble, C, Lonsdale, R, Li, A, Nett, N, Hoebenreich, S, Lingnau, J B, Wirtz, C, Fares, C, Hinrichs, H, Deege, A, Mulholland, A J, Nov, Y, Leys, D, Mclean, K, Munro, A & Reetz, M T 2018, ' P450-Catalyzed Regio-and Diastereoselective Steroid Hydroxylation: Efficient Directed Evolution Enabled by Mutability Landscaping ', ACS Catalysis, vol. 8, no. 4, pp. 3395-3410 . https://doi.org/10.1021/acscatal.8b00389

Cytochrome P450 monooxygenases play a crucial role in the biosynthesis of many natural products and in the human metabolism of numerous pharmaceuticals. This has inspired synthetic organic and medicinal chemists to exploit them as catalysts in regio-

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::376c458d57db555de36b041a9eb1e1bb
https://www.research.manchester.ac.uk/portal/en/publications/p450catalyzed-regio-and-diastereoselective-steroid-hydroxylation-efficient-directed-evolution-enabled-by-mutability-landscaping(e831d47c-8f3f-42d1-b78c-5a2e390dc964).html

Laboratory-Scale Hydroxylation of Steroids by P450

Autor: Sabrina, Hoebenreich, Martin, Spinck, Nathalie, Nett

Publikováno v: Methods in molecular biology (Clifton, N.J.). 1645

This chapter describes the asymmetric hydroxylation of steroids on laboratory preparative scale, using engineered variants of P450

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::71016cefbc88dbfe9396bc62a26694a6
https://pubmed.ncbi.nlm.nih.gov/28710633

Bioorthogonal Enzymatic Activation of Caged Compounds

Autor: Peter L. Graumann, Eric Meggers, Katja Kräling, Nathalie Nett, Manfred T. Reetz, Felix Dempwolff, Cornelia Ritter, Carlos G. Acevedo-Rocha, Richard Lonsdale, Sabrina Hoebenreich

Publikováno v: Angewandte Chemie (International ed. in English). 54(45)

Engineered cytochrome P450 monooxygenase variants are reported as highly active and selective catalysts for the bioorthogonal uncaging of propargylic and benzylic ether protected substrates, including uncaging in living E. coli. observed selectivity

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::31672f561bd92bc49fdb885a1a763a08
https://pubmed.ncbi.nlm.nih.gov/26356324