Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Natasha, Sienkiewicz"'
Publikováno v:
PLoS Neglected Tropical Diseases, Vol 10, Iss 5, p e0004714 (2016)
Bifunctional dihydrofolate reductase-thymidylate synthase (DHFR-TS) is a chemically and genetically validated target in African trypanosomes, causative agents of sleeping sickness in humans and nagana in cattle. Here we report the kinetic properties
Externí odkaz:
https://doaj.org/article/79e36dfa8ce84c4388fc01bd0e26d301
Autor:
Simon, Dewar, Natasha, Sienkiewicz, Han B, Ong, Richard J, Wall, David, Horn, Alan H, Fairlamb
Publikováno v:
The Journal of Biological Chemistry
The aim of this study was to identify and characterize mechanisms of resistance to antifolate drugs in African trypanosomes. Genome-wide RNAi library screens were undertaken in bloodstream form Trypanosoma brucei exposed to the antifolates methotrexa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::69e4d33d3954b330a305e64bafb10831
https://pubmed.ncbi.nlm.nih.gov/27703008
https://pubmed.ncbi.nlm.nih.gov/27703008
Publikováno v:
The Journal of Biological Chemistry
Leishmania parasites are pteridine auxotrophs that use an NADPH-dependent pteridine reductase 1 (PTR1) and NADH-dependent quinonoid dihydropteridine reductase (QDPR) to salvage and maintain intracellular pools of tetrahydrobiopterin (H(4)B). However,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d662e37ff3038e7deabdc2ed87a38c96
https://doi.org/10.1074/jbc.m110.209593
https://doi.org/10.1074/jbc.m110.209593
Autor:
Natasha Sienkiewicz, Alan H. Fairlamb, David A. Robinson, Han B. Ong, Julie A. Frearson, Emma Shanks, Stephen Thompson
Publikováno v:
Analytical Biochemistry
Activity of the pterin- and folate-salvaging enzymes pteridine reductase 1 (PTR1) and dihydrofolate reductase–thymidylate synthetase (DHFR-TS) is commonly measured as a decrease in absorbance at 340nm, corresponding to oxidation of nicotinamide ade
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::525c880b1809f86b3af13e64675c07fd
https://doi.org/10.1016/j.ab.2009.09.003
https://doi.org/10.1016/j.ab.2009.09.003
Publikováno v:
Molecular Microbiology
Summary African trypanosomes are capable of both de novo synthesis and salvage of pyrimidines. The last two steps in de novo synthesis are catalysed by UMP synthase (UMPS) - a bifunctional enzyme comprising orotate phosphoribosyl transferase (OPRT) a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aba490857f4ccef28d0b891a73f66aaa
https://pubmed.ncbi.nlm.nih.gov/23980694
https://pubmed.ncbi.nlm.nih.gov/23980694
Publikováno v:
Molecular Microbiology
The phenotypes of single- (SKO) and double-knockout (DKO) lines of dihydrofolate reductase–thymidylate synthase (DHFR–TS) of bloodstream Trypanosoma brucei were evaluated in vitro and in vivo. Growth of SKO in vitro is identical to wild-type (WT)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a63dc9635a0bcb2f10cbd527c5a6b8f4
https://pubmed.ncbi.nlm.nih.gov/18557814
https://pubmed.ncbi.nlm.nih.gov/18557814
Autor:
Alice Dawson, Karen McLuskey, Paul K. Fyfe, Lindsay B. Tulloch, William N. Hunter, Federica Gibellini, Natasha Sienkiewicz, Alan H. Fairlamb
Publikováno v:
Molecular Microbiology
The protozoan Trypanosoma brucei has a functional pteridine reductase (TbPTR1), an NADPH-dependent short-chain reductase that participates in the salvage of pterins, which are essential for parasite growth. PTR1 displays broad-spectrum activity with
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fe4d88abf502bef9fd6fb6707af9d2e7
https://pubmed.ncbi.nlm.nih.gov/16968221
https://pubmed.ncbi.nlm.nih.gov/16968221
Autor:
Daniel Dive, Eric Viscogliosi, René Wintjens, Jamal Khalife, Wassim Daher, Sylke Müller, Hélène Jouin, Monique Capron, Carsten Wrenger, Natasha Sienkiewicz
Publikováno v:
Molecular and biochemical parasitology. 137(1)
The intraerythrocytic stages of Plasmodium falciparum are exposed to oxidative stress and require functional anti-oxidant systems to survive. In addition to the parasite's known iron-dependent superoxide dismutase PfSOD1, a second SOD gene (PfSOD2) i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4f41eab2a8f80463034da7e424863d78
https://pubmed.ncbi.nlm.nih.gov/15279958
https://pubmed.ncbi.nlm.nih.gov/15279958