Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Natalya Kouprina"'
Autor:
Ronald E. Cannon, Joan P. Graves, Vladimir Larionov, Natalya Kouprina, Vladimir N. Noskov, Raymond W. Tennant, Michael C. Humble, Michael A. Resnick, Ed Garner
Publikováno v:
Genomics. 70:292-299
Transformation-associated recombination (TAR) cloning allows entire genes and large chromosomal regions to be specifically, accurately, and quickly isolated from total genomic DNA. We report the first example of radial TAR cloning from the mouse geno
Autor:
Vladimir N. Noskov, Lisa Stubbs, Xiaochen Lu, Joomyeong Kim, Anne Bergmann, Paul G. Richardson, Xiaojia Ren, Natalya Kouprina, Tiffany Warth
Publikováno v:
Genome Research. 10:1138-1147
Although the two alleles of most mammalian autosomal genes are functionally equivalent, the maternal and paternal copies of a subset of genes are distinguished by genomic imprinting, a mechanism by which one allele is epigenetically modified and repr
Autor:
Greg Solomon, LouAnn Cable, Vladimir Larionov, David E. Hill, Natalya Kouprina, J. Carl Barrett, Cynthia A. Afshari, Lois A. Annab
Publikováno v:
Gene. 250:201-208
The BRCA1 gene, mutations of which contribute significantly to hereditary breast cancer, was not identified in the existing YAC and BAC libraries. The gene is now available only as a set of overlapping fragments that form a contig. In this work we de
Publikováno v:
Proceedings of the National Academy of Sciences. 93:13925-13930
T ransformation- a ssociated r ecombination (TAR) can be exploited in yeast to clone human DNAs. TAR cloning was previously accomplished using one or two telomere-containing vectors with a common human repeat(s) that could recombine with human DNA du
Autor:
Roger H. Reeves, Vladimir Larionov, Kellie M. Tainton, Michael A. Resnick, Natalya Kouprina, Joan P. Graves, K. H. A. Choo, Michael R. Cancilla, L. E. Matesic
Publikováno v:
Mammalian Genome. 9:157-159
Several yeast artificial chromosome (YAC) libraries have been constructed for physical mapping and characterization of the mouse genome (Larin et al. 1991; Chartier et al. 1992; Haldi et al. 1996). These complex libraries were constructed by in vitro
Autor:
Naoki, Nihei, Natalya, Kouprina, Vladimir, Larionov, Junko, Oshima, George M, Martin, Tomohiko, Ichikawa, J Carl, Barrett
Publikováno v:
Cancer research. 62(2)
We recently demonstrated that the human chromosome 8p21-p12 region encodes a metastasis suppressor gene for rat prostate cancer. The presence of this region suppresses the metastatic ability of rat prostate cancer cells (N. Nihei et al., Genes Chromo
Autor:
Joan P. Graves, Robert K. Moyzis, Judith G. Tesmer, Mary L. Campbell, Vladimir Larionov, Linda Meincke, Natalya Kouprina, Deborah L. Grady, Norman A. Doggett, Larry L. Deaven, E.W. Campbell
Publikováno v:
Genomics. 53(1)
Transformation-associated recombination (TAR) in yeast was exploited for the selective isolation of human DNAs as large circular yeast artificial chromosomes (YACs) from two rodent/human hybrid cell lines containing human chromosomes 5 and 16. TAR cl
Publikováno v:
Genome research. 8(6)
In contrast to other animal cell lines, the chicken pre-B cell lymphoma line, DT40, exhibits a high level of homologous recombination, which can be exploited to generate site-specific alterations in defined target genes or regions. In addition, the a
Autor:
Michael A. Resnick, K. H. Andy Choo, Natalya Kouprina, Michael R. Cancilla, Alyssa E. Barry, Vladimir Larionov, Kellie M. Tainton, Desirée du Sart
Publikováno v:
Genomics. 47(3)
The transformation-associated recombination (TAR) procedure allows rapid, site-directed cloning of specific human chromosomal regions as yeast artificial chromosomes (YACs). The procedure requires knowledge of only a single, relatively small genomic
Publikováno v:
Gene. 197(1-2)
Selective cloning of human DNA in YACs from monochromosomal human/rodent hybrid cells lines and radiation hybrids can be accomplished by transformation-associated recombination (TAR) between Alu-containing vector(s) and human DNA in yeast. We have ex