Host factor prioritization for pan-viral genetic perturbation screens using random intercept models and network propagation.

Autor: Simon Dirmeier, Christopher Dächert, Martijn van Hemert, Ali Tas, Natacha S Ogando, Frank van Kuppeveld, Ralf Bartenschlager, Lars Kaderali, Marco Binder, Niko Beerenwinkel

Publikováno v: PLoS Computational Biology, Vol 16, Iss 2, p e1007587 (2020)

Genetic perturbation screens using RNA interference (RNAi) have been conducted successfully to identify host factors that are essential for the life cycle of bacteria or viruses. So far, most published studies identified host factors primarily for si

Externí odkaz: https://doaj.org/article/6707f2e8b6504605b58994239a1c56b2

Impact of changes in human airway epithelial cellular composition and differentiation on SARS-CoV-2 infection biology

Autor: Melissa Thaler, Ying Wang, Anne M. van der Does, Alen Faiz, Dennis K. Ninaber, Natacha S. Ogando, Hendrik Beckert, Christian Taube, Clarisse Salgado-Benvindo, Eric J. Snijder, Peter J. Bredenbeek, Pieter S. Hiemstra, Martijn J. van Hemert

Publikováno v: Journal of Innate Immunity.

in press, CA extern The consequences of infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can range from asymptomatic to fatal disease. Variations in epithelial susceptibility to SARS-CoV-2 infection depend on the anatomical

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2d3d975436d08634c326f0379efd6f40
https://doi.org/10.1159/000530374

Potent Inhibition of SARS-CoV-2 nsp14 N 7-Methyltransferase by Sulfonamide-Based Bisubstrate Analogues

Autor: Rostom Ahmed-Belkacem, Marcel Hausdorff, Adrien Delpal, Priscila Sutto-Ortiz, Agathe M. G. Colmant, Franck Touret, Natacha S. Ogando, Eric J. Snijder, Bruno Canard, Bruno Coutard, Jean-Jacques Vasseur, Etienne Decroly, Françoise Debart

Publikováno v: Journal of Medicinal Chemistry
Journal of Medicinal Chemistry, 2022, 65 (8), pp.6231-6249. ⟨10.1021/acs.jmedchem.2c00120⟩
Journal of Medicinal Chemistry, 65(8), 6231-6249. AMER CHEMICAL SOC

International audience; Enzymes involved in RNA capping of SARS-CoV-2 are essential for the stability of viral RNA, translation of mRNAs, and virus evasion from innate immunity, making them attractive targets for antiviral agents. In this work, we fo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::662d59e717710253e38775facf4525bd
https://hal.science/hal-03721688

Structure-function analysis of the nsp14 N7-guanine methyltransferase reveals an essential role in Betacoronavirus replication

Autor: Volker Thiel, Jessika C. Zevenhoven-Dobbe, Clara C. Posthuma, Priscila El Kazzi, Eric J. Snijder, Alice Decombe, François Ferron, Natacha S. Ogando, Etienne Decroly, Bruno Canard, Brenda w. Bontes

Publikováno v: Proceedings of the National Academy of Sciences, 118(49). NATL ACAD SCIENCES
Ogando, Natacha S; El Kazzi, Priscila; Zevenhoven-Dobbe, Jessika C; Bontes, Brenda W; Decombe, Alice; Posthuma, Clara C; Thiel, Volker; Canard, Bruno; Ferron, François; Decroly, Etienne; Snijder, Eric J (2021). Structure-function analysis of the nsp14 N7-guanine methyltransferase reveals an essential role in Betacoronavirus replication. Proceedings of the National Academy of Sciences of the United States of America-PNAS, 118(49) National Academy of Sciences NAS 10.1073/pnas.2108709118
Proceedings of the National Academy of Sciences of the United States of America
Proceedings of the National Academy of Sciences of the United States of America, 2021, ⟨10.1101/2021.05.17.444407⟩

As coronaviruses (CoVs) replicate in the host cell cytoplasm, they rely on their own capping machinery to ensure the efficient translation of their mRNAs, protect them from degradation by cellular 5’ exoribonucleases, and escape innate immune sensi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::156d64145c316b53403328c37d9acc11
https://hal-amu.archives-ouvertes.fr/hal-03426119/file/2021.05.17.444407.full.pdf

Impact of human airway epithelial cellular composition on SARS-CoV-2 infection biology

Autor: Peter J. Bredenbeek, Dennis K. Ninaber, Clarisse Salgado Benvindo Da Silva, Christian Taube, Martijn J. van Hemert, Natacha S. Ogando, Pieter S. Hiemstra, Ying Wang, Hendrik Beckert, Anne M. van der Does, Melissa Thaler, Eric J. Snijder

Infection biology and pathogenesis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of the coronavirus disease 2019 (COVID-19), are incompletely understood. Here, we assessed the impact of airway epithelial cellula

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::79f65cc8ce1743a7b2761d8f18a90d39
https://doi.org/10.1101/2021.07.21.453304