Preparation of oxygen-sensitive proteins for high-resolution cryoEM structure determination using (an)aerobic blot-free vitrification.

Autor: Cook BD; Department of Chemistry and Biochemistry, University of California, San Diego, California, USA., Narehood SM; Department of Chemistry and Biochemistry, University of California, San Diego, California, USA., McGuire KL; Department of Chemistry and Biochemistry, University of California, San Diego, California, USA., Li Y; Department of Chemistry and Biochemistry, University of California, San Diego, California, USA., Tezcan FA; Department of Chemistry and Biochemistry, University of California, San Diego, California, USA., Herzik MA Jr; Department of Chemistry and Biochemistry, University of California, San Diego, California, USA.

Publikováno v: BioRxiv : the preprint server for biology [bioRxiv] 2024 Jul 22. Date of Electronic Publication: 2024 Jul 22.

The Laser-Induced Potential Jump: A Method for Rapid Electron Injection into Oxidoreductase Enzymes.

Autor: Sanchez MLK; Department of Chemistry, Emory University, Atlanta, Georgia 30030, United States., Konecny SE; Department of Chemistry, Emory University, Atlanta, Georgia 30030, United States., Narehood SM; Department of Chemistry, Emory University, Atlanta, Georgia 30030, United States., Reijerse EJ; Max Planck Institute for Chemical Energy Conversion, Stiftstrasse 34-36, Mülheim an der Ruhr 45470, Germany., Lubitz W; Max Planck Institute for Chemical Energy Conversion, Stiftstrasse 34-36, Mülheim an der Ruhr 45470, Germany., Birrell JA; Max Planck Institute for Chemical Energy Conversion, Stiftstrasse 34-36, Mülheim an der Ruhr 45470, Germany., Dyer RB; Department of Chemistry, Emory University, Atlanta, Georgia 30030, United States.

Publikováno v: The journal of physical chemistry. B [J Phys Chem B] 2020 Oct 08; Vol. 124 (40), pp. 8750-8760. Date of Electronic Publication: 2020 Sep 29.